Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F73 I1 R1	33	77.0	2636344	99.1%	2612616	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,497,700	C→A	pseudogene (951/1191 nt)	intB →	KpLE2 phage‑like element; putative integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,497,700	0	C	A	99.2%	435.8 / ‑3.9	122	pseudogene (951/1191 nt)	intB	KpLE2 phage‑like element; putative integrase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (75/46); minor base . (1/0); total (76/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

Only 100 of 123 total aligned reads displayed.

ATAAAACAGTTTTATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGCTTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAA  >  NC_000913/4497563‑4497835

       aGTTTTATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACa                                                                                                                                >  2:730116/1‑140 (MQ=255)

        gTTTTATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACaa                                                                                                                               <  2:151264/140‑1 (MQ=255)

         ttttATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCACACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAAt                                                                                                                              >  1:911869/1‑140 (MQ=255)

          tttATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATg                                                                                                                             >  2:435059/1‑140 (MQ=255)

          tttATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATg                                                                                                                             <  2:477448/140‑1 (MQ=255)

          tttATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATg                                                                                                                             >  2:1148613/1‑140 (MQ=255)

           ttATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATgg                                                                                                                            >  2:1239578/1‑140 (MQ=255)

            tATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGc                                                                                                                           <  1:779642/140‑1 (MQ=255)

            tATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGc                                                                                                                           <  1:707269/140‑1 (MQ=255)

            tATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGc                                                                                                                           <  1:960415/140‑1 (MQ=255)

                   cccATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACaa                                                                                                                               <  2:219059/129‑1 (MQ=255)

                   cccATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACaa                                                                                                                               >  1:219059/1‑129 (MQ=255)

                     cATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTc                                                                                                                  >  2:44960/1‑140 (MQ=255)

                      aTGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCa                                                                                                                 <  1:754215/140‑1 (MQ=255)

                      aTGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCa                                                                                                                 <  1:730116/140‑1 (MQ=255)

                           ttGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGt                                                                                                            <  1:597124/140‑1 (MQ=255)

                           ttGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGt                                                                                                            <  1:924220/140‑1 (MQ=255)

                             gATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCg                                                                                                          >  2:358973/1‑140 (MQ=255)

                             gATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCg                                                                                                          >  2:457312/1‑140 (MQ=255)

                             gATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCg                                                                                                          >  2:320496/1‑140 (MQ=255)

                                atttattGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAAt                                                                                                       <  2:78063/140‑1 (MQ=255)

                                   tattGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAg                                                                                                    >  2:220033/1‑140 (MQ=255)

                                   tattGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAg                                                                                                    >  1:1069384/1‑140 (MQ=255)

                                    attGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAgg                                                                                                   >  2:379584/1‑140 (MQ=255)

                                        gTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCtg                                                                                               >  2:890927/1‑140 (MQ=255)

                                         tGATCAAGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCtgt                                                                                              >  2:760853/1‑140 (MQ=255)

                                           aTCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTgg                                                                                            <  1:457312/140‑1 (MQ=255)

                                           aTCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTgg                                                                                            >  1:334541/1‑140 (MQ=255)

                                           aTCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTgg                                                                                            >  2:425297/1‑140 (MQ=255)

                                            tCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGt                                                                                           <  1:509099/140‑1 (MQ=255)

                                            tCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTAAGGGTCATGGGGAATGATAAAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTggg                                                                                           >  2:1233677/1‑139 (MQ=255)

                                                    cGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGAt                                                                                   <  1:320496/140‑1 (MQ=255)

                                                          aaCCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGtt                                                                             >  2:896047/1‑140 (MQ=255)

                                                                    tgagAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGa                                                                   >  1:812742/1‑140 (MQ=255)

                                                                     gagaATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGAGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGCGATGCTGTTGAACGTCAGAt                                                                  >  1:834899/1‑140 (MQ=255)

                                                                          tACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGcc                                                             >  1:903796/1‑140 (MQ=255)

                                                                            cGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCcac                                                           >  1:1049169/1‑140 (MQ=255)

                                                                            cGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCcac                                                           >  2:806860/1‑140 (MQ=255)

                                                                             ggTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGACTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCcaca                                                          >  2:816189/1‑140 (MQ=255)

                                                                             ggTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGATTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGCCTCGTGATGCTGTTGAACGTCAGATGAGCcaca                                                          >  2:833092/1‑140 (MQ=255)

                                                                              gTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACAt                                                         >  1:161325/1‑140 (MQ=255)

                                                                                aaaTAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATgg                                                       >  2:576717/1‑140 (MQ=255)

                                                                                  aTAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGgcg                                                     >  2:440476/1‑140 (MQ=255)

                                                                                  aTAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGgcg                                                     >  2:30226/1‑140 (MQ=255)

                                                                                   tAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGgcgc                                                    <  2:903796/140‑1 (MQ=255)

                                                                                    aGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGcgcg                                                   <  1:730873/140‑1 (MQ=255)

                                                                                    aGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGcgcg                                                   <  1:989187/140‑1 (MQ=255)

                                                                                     gTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGa                                                  >  2:1147916/1‑140 (MQ=255)

                                                                                      tGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGaa                                                 >  2:17145/1‑140 (MQ=255)

                                                                                      tGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGaa                                                 >  2:553882/1‑140 (MQ=255)

                                                                                       gCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGaaa                                                >  1:889671/1‑140 (MQ=255)

                                                                                       gCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGaaa                                                >  2:297619/1‑140 (MQ=255)

                                                                                       gCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGaaa                                                >  1:592497/1‑140 (MQ=255)

                                                                                       gCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGG‑‑ATCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAAtt                                              >  1:173780/1‑140 (MQ=255)

                                                                                        cGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGgc                                                      >  1:25251/1‑133 (MQ=255)

                                                                                        cGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAAt                                               >  2:257401/1‑140 (MQ=255)

                                                                                        cGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAAt                                               >  2:401152/1‑140 (MQ=255)

                                                                                        cGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAAt                                               >  2:495065/1‑140 (MQ=255)

                                                                                        cGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAAt                                               >  2:1151599/1‑140 (MQ=255)

                                                                                         gTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAAtt                                              >  2:500408/1‑140 (MQ=255)

                                                                                           tACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAAt                                               <  1:599948/137‑1 (MQ=255)

                                                                                             cGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGt                                          <  2:916889/140‑1 (MQ=255)

                                                                                             cGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGt                                          <  1:890927/140‑1 (MQ=255)

                                                                                                   aTGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGc                                    <  1:440476/140‑1 (MQ=255)

                                                                                                        gTATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGt                               <  1:576717/140‑1 (MQ=255)

                                                                                                         tATGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGta                              >  1:1056299/1‑140 (MQ=255)

                                                                                                          aTGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGtat                             <  2:334541/140‑1 (MQ=255)

                                                                                                          aTGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGtat                             <  1:806860/140‑1 (MQ=255)

                                                                                                           tGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGta                              <  1:327218/138‑1 (MQ=255)

                                                                                                           tGATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGta                              >  2:327218/1‑138 (MQ=255)

                                                                                                            gATACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTatat                           >  1:491864/1‑140 (MQ=255)

                                                                                                             aTACAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATc                          >  2:1284156/1‑140 (MQ=255)

                                                                                                               aCAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCa                        <  2:911869/140‑1 (MQ=255)

                                                                                                               aCAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCa                        <  2:1069384/140‑1 (MQ=255)

                                                                                                               aCAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCa                        <  1:256544/140‑1 (MQ=255)

                                                                                                                cAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCAt                       >  1:933215/1‑140 (MQ=255)

                                                                                                                cAAAAGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATACAt                       >  1:1242569/1‑140 (MQ=255)

                                                                                                                 aaaaGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATa                      >  2:356588/1‑140 (MQ=255)

                                                                                                                 aaaaGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATa                      <  1:425297/140‑1 (MQ=255)

                                                                                                                   aaGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATaaa                    >  2:706243/1‑140 (MQ=255)

                                                                                                                   aaGTAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATaaa                    >  2:708314/1‑140 (MQ=255)

                                                                                                                      tAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGtat                             <  1:1072437/128‑1 (MQ=255)

                                                                                                                      tAGAGGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGtat                             >  2:1072437/1‑128 (MQ=255)

                                                                                                                       agagGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCag                <  2:1049169/140‑1 (MQ=255)

                                                                                                                       agagGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCag                >  2:723114/1‑140 (MQ=255)

                                                                                                                        gagGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCaga               <  1:1239578/140‑1 (MQ=255)

                                                                                                                        gagGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCaga               >  2:922495/1‑140 (MQ=255)

                                                                                                                        gagGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCaga               >  1:1029315/1‑140 (MQ=255)

                                                                                                                        gagGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCaga               >  2:1282566/1‑140 (MQ=255)

                                                                                                                        gagGTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCaga               >  2:159093/1‑140 (MQ=255)

                                                                                                                           gTTTGTGGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCa            >  2:884978/1‑140 (MQ=255)

                                                                                                                              tgtgGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAg                   <  1:898226/130‑1 (MQ=255)

                                                                                                                              tgtgGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAg                   >  2:898226/1‑130 (MQ=255)

                                                                                                                              tgtgGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCt         >  1:1206290/1‑140 (MQ=255)

                                                                                                                               gtgGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTg        >  2:520651/1‑140 (MQ=255)

                                                                                                                               gtgGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTg        >  2:200477/1‑140 (MQ=255)

                                                                                                                                tgGTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTgg       >  1:936992/1‑140 (MQ=255)

                                                                                                                                 ggTCATGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGga      <  1:358973/140‑1 (MQ=255)

                                                                                                                                     aTGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGgaagaa  >  2:480815/1‑140 (MQ=255)

                                                                                                                                     aTGGATTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGgaagaa  >  1:1167900/1‑140 (MQ=255)

ATAAAACAGTTTTATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGCTTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAA  >  NC_000913/4497563‑4497835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A