Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	975,711	A→G	L30L (TTA→TTG)	mukE →	chromosome partitioning protein MukE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	975,711	0	A	G	100.0%	198.5 / NA	53	L30L (TTA→TTG)	mukE	chromosome partitioning protein MukE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/37); total (16/37)

GCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATT  >  NC_000913/975576‑975844

gCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGtt                                                                                                                                   <  1:104996/140‑1 (MQ=255)

  gAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCa                                                                                                                                 <  2:650180/140‑1 (MQ=255)

  gAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCa                                                                                                                                 <  1:24191/140‑1 (MQ=255)

     aTGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTGCAGGa                                                                                                                              <  2:424956/140‑1 (MQ=255)

                 tAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGc                                                                                                                  <  2:284433/140‑1 (MQ=255)

                      aTTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTcga                                                                                                             >  1:645275/1‑140 (MQ=255)

                        tACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTcgacg                                                                                                           <  2:786470/140‑1 (MQ=255)

                        tACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTcgacg                                                                                                           <  1:621497/140‑1 (MQ=255)

                          cGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGaa                                                                                                         <  2:146320/140‑1 (MQ=255)

                              gCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTgg                                                                                                     >  2:39937/1‑140 (MQ=255)

                              gCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTgg                                                                                                     >  2:770647/1‑140 (MQ=255)

                               ccAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGa                                                                                                    <  2:698306/140‑1 (MQ=255)

                                  aGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATaa                                                                                                 >  2:702076/1‑140 (MQ=255)

                                         ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCa                                                                                          <  1:302765/140‑1 (MQ=255)

                                         ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCa                                                                                          >  2:664885/1‑140 (MQ=255)

                                           cgcATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCAtt                                                                                        >  1:892620/1‑140 (MQ=255)

                                             cATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTcc                                                                                      >  2:677952/1‑140 (MQ=255)

                                               tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTg                                                                                    >  2:454661/1‑140 (MQ=255)

                                               tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTg                                                                                    >  2:373803/1‑140 (MQ=255)

                                               tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTg                                                                                    >  1:939631/1‑140 (MQ=255)

                                               tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTg                                                                                    >  2:140667/1‑140 (MQ=255)

                                                       aCAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGAttt                                                                            <  2:198675/140‑1 (MQ=255)

                                                         aaaTATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTc                                                                          <  2:21024/140‑1 (MQ=255)

                                                             atTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGa                                                                      <  1:220163/140‑1 (MQ=255)

                                                                      gTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGa                                                             >  1:426971/1‑140 (MQ=255)

                                                                       tGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGaa                                                            <  2:849730/140‑1 (MQ=255)

                                                                           gCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGt                                                        >  2:549096/1‑140 (MQ=255)

                                                                              ggTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGtttt                                                     <  2:210998/140‑1 (MQ=255)

                                                                                ttAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAc                                                   <  1:756278/140‑1 (MQ=255)

                                                                                 tAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcg                                                  <  1:236773/140‑1 (MQ=255)

                                                                                 tAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcg                                                  <  1:454661/140‑1 (MQ=255)

                                                                                  aaGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgc                                                 <  1:140667/140‑1 (MQ=255)

                                                                                   aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  2:645275/140‑1 (MQ=255)

                                                                                   aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  2:822558/140‑1 (MQ=255)

                                                                                   aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  2:945743/140‑1 (MQ=255)

                                                                                   aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  1:1007015/140‑1 (MQ=255)

                                                                                   aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  1:635310/140‑1 (MQ=255)

                                                                                        gtgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  2:561929/138‑1 (MQ=255)

                                                                                        gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  1:770647/140‑1 (MQ=255)

                                                                                        gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  2:892620/140‑1 (MQ=255)

                                                                                        gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  1:39937/140‑1 (MQ=255)

                                                                                        gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  1:780402/140‑1 (MQ=255)

                                                                                         cgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTtat                                          <  1:677952/140‑1 (MQ=255)

                                                                                             ggCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACg                                      >  2:106509/1‑140 (MQ=255)

                                                                                              gcgcTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGt                                     >  1:868684/1‑140 (MQ=255)

                                                                                                          ccGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcg                         <  1:867737/140‑1 (MQ=255)

                                                                                                          ccGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcg                         <  2:616808/140‑1 (MQ=255)

                                                                                                            gTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcgcg                       <  2:680349/140‑1 (MQ=255)

                                                                                                                  tCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAg                 <  1:549096/140‑1 (MQ=255)

                                                                                                                    cGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGaa               <  1:373803/140‑1 (MQ=255)

                                                                                                                       cgcTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAggg            <  2:929477/140‑1 (MQ=255)

                                                                                                                            gACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGttctt       <  1:664885/140‑1 (MQ=255)

                                                                                                                                 cgcCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTAtt  >  2:363242/1‑140 (MQ=255)

GCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATT  >  NC_000913/975576‑975844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A