Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,259,759	A→G	S349P (TCC→CCC)	dauA ←	aerobic C4‑dicarboxylate transporter DauA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,259,759	0	A	G	100.0%	164.6 / NA	46	S349P (TCC→CCC)	dauA	aerobic C4‑dicarboxylate transporter DauA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/20); total (26/20)

TTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCG  >  NC_000913/1259628‑1259892

ttCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGccc                                                                                                                               >  2:36076/1‑140 (MQ=255)

 tCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGcccc                                                                                                                              >  1:504131/1‑140 (MQ=255)

       aTGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACg                                                                                                                        <  2:453268/140‑1 (MQ=255)

          ttCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGAc                                                                                                                     >  1:873295/1‑140 (MQ=255)

             cACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGtt                                                                                                                  >  1:911358/1‑140 (MQ=255)

               cGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAg                                                                                                                >  1:108565/1‑140 (MQ=255)

               cGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAg                                                                                                                >  2:420652/1‑140 (MQ=255)

                   accaTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGc                                                                                                            >  1:356275/1‑140 (MQ=255)

                   accaTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGc                                                                                                            >  2:179413/1‑140 (MQ=255)

                              cagGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGa                                                                                                 >  1:337307/1‑140 (MQ=255)

                              cagGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGa                                                                                                 >  1:1028673/1‑140 (MQ=255)

                                 ggCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATgg                                                                                              <  1:460148/140‑1 (MQ=255)

                                      ccATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCt                                                                                         <  1:449137/140‑1 (MQ=255)

                                        aTGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGTCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGt                                                                                       <  2:514688/140‑1 (MQ=255)

                                           gCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGc                                                                                    >  2:380802/1‑140 (MQ=255)

                                           gCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGc                                                                                    >  1:536049/1‑140 (MQ=255)

                                            cGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCg                                                                                   <  2:979864/140‑1 (MQ=255)

                                                     gCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATacca                                                                          >  2:834474/1‑140 (MQ=255)

                                                     gCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATacca                                                                          <  1:543043/140‑1 (MQ=255)

                                                             gggagCAGCGGTGCCAGTCCCAGCAGGGCAAGAATAACCAGAATAGAGGGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGaa                                                                  <  2:93917/138‑1 (MQ=255)

                                                              agagCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAAc                                                                 <  2:122997/140‑1 (MQ=255)

                                                               gagCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACg                                                                <  1:420652/140‑1 (MQ=255)

                                                                  cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                             >  2:923782/1‑140 (MQ=255)

                                                                  cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                             <  2:289772/140‑1 (MQ=255)

                                                                  cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                             <  2:710459/140‑1 (MQ=255)

                                                                  cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGCGGGCGCTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                             >  2:580940/1‑140 (MQ=255)

                                                                     cGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGa                                                          >  1:971353/1‑140 (MQ=255)

                                                                          ccAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGataata                                                     <  2:945908/140‑1 (MQ=255)

                                                                          ccAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGataata                                                     <  1:852151/140‑1 (MQ=255)

                                                                               aCCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTccc                                                >  1:913471/1‑140 (MQ=255)

                                                                                    cagGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTc                                           <  1:179413/140‑1 (MQ=255)

                                                                                    cagGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTc                                           >  2:780421/1‑140 (MQ=255)

                                                                                    cagGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTc                                           <  2:1028673/140‑1 (MQ=255)

                                                                                    cagGGCAAGAAAAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTc                                           <  1:111870/140‑1 (MQ=255)

                                                                                      gggCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGTCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTccc                                         <  2:754355/140‑1 (MQ=255)

                                                                                                 aCCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAg                              >  1:411172/1‑140 (MQ=255)

                                                                                                             tGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGc                  >  1:304331/1‑140 (MQ=255)

                                                                                                                aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               >  2:515776/1‑140 (MQ=255)

                                                                                                                aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               >  2:597081/1‑140 (MQ=255)

                                                                                                                aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               >  2:715880/1‑140 (MQ=255)

                                                                                                                aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               >  1:559695/1‑140 (MQ=255)

                                                                                                                aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               <  2:108565/140‑1 (MQ=255)

                                                                                                                     cgccgAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGtt          >  2:496432/1‑140 (MQ=255)

                                                                                                                     cgccgAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATCGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGtt          >  2:771106/1‑140 (MQ=255)

                                                                                                                          agaTAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGTACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTc     <  2:820899/140‑1 (MQ=255)

                                                                                                                             tAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCg  <  1:988175/140‑1 (MQ=255)

TTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCG  >  NC_000913/1259628‑1259892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A