Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,133	C→T	L142L (TTG→TTA)	btuD ←	vitamin B12 ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,133	0	C	T	100.0%	150.7 / NA	46	L142L (TTG→TTA)	btuD	vitamin B12 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/18); total (28/18)

ATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGC  >  NC_000913/1792996‑1793265

atcaCAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTaa                                                                                                                                    >  1:446956/1‑140 (MQ=255)

      aTCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAAcaccac                                                                                                                              <  1:888895/140‑1 (MQ=255)

           cAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCgcag                                                                                                                         <  2:513033/140‑1 (MQ=255)

                   gCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGt                                                                                                                 >  1:801828/1‑140 (MQ=255)

                      gACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCGAGACGTAcg                                                                                                              <  1:635529/140‑1 (MQ=255)

                        cacaGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGCCGTAcgcg                                                                                                            >  1:39206/1‑140 (MQ=255)

                        cacaGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTAcgcg                                                                                                            <  1:101271/140‑1 (MQ=255)

                          caGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGtt                                                                                                          >  2:694400/1‑140 (MQ=255)

                            gcgcgcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGc                                                                                                        >  2:19451/1‑140 (MQ=255)

                            gcgcgcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGc                                                                                                        >  2:337310/1‑140 (MQ=255)

                             cgcgcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGcc                                                                                                       >  2:830517/1‑140 (MQ=255)

                             cgcgcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGcc                                                                                                       >  1:962865/1‑140 (MQ=255)

                                gcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCAtt                                                                                                    <  2:530203/140‑1 (MQ=255)

                                 cTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTc                                                                                                   >  1:240437/1‑140 (MQ=255)

                                  tCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTAcg                                                                                                              <  2:195821/128‑1 (MQ=255)

                                  tCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTAcg                                                                                                              >  1:195821/1‑128 (MQ=255)

                                  tCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCa                                                                                                  <  2:873553/140‑1 (MQ=255)

                                                     tcTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCCGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTgg                                                                               <  2:467995/139‑1 (MQ=255)

                                                             gcgcAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGt                                                                       <  1:436589/140‑1 (MQ=255)

                                                               gcAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTcc                                                                     <  2:1006153/140‑1 (MQ=255)

                                                                   cATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCGCCGCCGGAAAGTTGATTGGTGCTACGTCCGAGt                                                                 >  2:887281/1‑140 (MQ=255)

                                                                             gTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCaa                                                       >  2:113784/1‑140 (MQ=255)

                                                                              ttCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAag                                                      >  1:115014/1‑140 (MQ=255)

                                                                              ttCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAag                                                      >  1:933874/1‑140 (MQ=255)

                                                                              ttCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAag                                                      >  2:786100/1‑140 (MQ=255)

                                                                                 aTCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGc                                                   >  2:885854/1‑140 (MQ=255)

                                                                                   cGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCa                                                 >  1:102647/1‑140 (MQ=255)

                                                                                   cGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGCTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCa                                                 >  1:860449/1‑140 (MQ=255)

                                                                                     gCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAgc                                               <  1:127227/140‑1 (MQ=255)

                                                                                        catcaAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGcc                                            >  2:1023104/1‑140 (MQ=255)

                                                                                        catcaAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGcc                                            >  2:37083/1‑140 (MQ=255)

                                                                                            aagaagCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTg                                        <  1:887281/140‑1 (MQ=255)

                                                                                            aagaagCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTg                                        <  1:571705/140‑1 (MQ=255)

                                                                                               aagCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGa                                     <  1:830517/140‑1 (MQ=255)

                                                                                                agcagcAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAc                                    >  2:338118/1‑140 (MQ=255)

                                                                                                 gcagcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAca                                   <  2:240437/140‑1 (MQ=255)

                                                                                                   agcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAcatc                                 >  2:467210/1‑140 (MQ=255)

                                                                                                   agcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAcatc                                 >  1:216128/1‑140 (MQ=255)

                                                                                                   agcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAcatc                                 >  1:341646/1‑140 (MQ=255)

                                                                                                    gcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAcatca                                <  2:39206/140‑1 (MQ=255)

                                                                                                      aaTTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCAtt                              <  2:993808/140‑1 (MQ=255)

                                                                                                               gCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACATCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTc                     >  1:787088/1‑140 (MQ=255)

                                                                                                                            gtggtgTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATCCAGTAGTTCGGTACGCGTTTTa        <  1:744706/140‑1 (MQ=255)

                                                                                                                             tggtgTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTAt       >  1:440170/1‑140 (MQ=255)

                                                                                                                               gtgtGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCg     >  1:534756/1‑140 (MQ=255)

                                                                                                                               gtgtGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCg     >  1:560470/1‑140 (MQ=255)

                                                                                                                                  tgATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGc  >  2:236177/1‑140 (MQ=255)

ATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGC  >  NC_000913/1792996‑1793265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A