Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,906,870	G→A	A64V (GCC→GTC)	rlmA ←	23S rRNA m(1)G745 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,906,870	0	G	A	100.0%	186.7 / NA	55	A64V (GCC→GTC)	rlmA	23S rRNA m(1)G745 methyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/30); total (25/30)

GGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGAC  >  NC_000913/1906738‑1906999

ggCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTaa                                                                                                                            >  2:696745/1‑140 (MQ=255)

    aCGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAAt                                                                                                                        >  1:179484/1‑140 (MQ=255)

    aCGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAAt                                                                                                                        >  2:1036653/1‑140 (MQ=255)

       cATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATACCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATgcg                                                                                                                     <  2:599397/140‑1 (MQ=255)

          cGGCAAATGCGTGTGTGTAATACCCTTCACCACGGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCgcgg                                                                                                                  <  1:516176/140‑1 (MQ=255)

           ggCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCgcggc                                                                                                                 >  2:627448/1‑140 (MQ=255)

             cAAATGCGTGTGTGTAATACCCTCCCCCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGt                                                                                                               <  2:31408/140‑1 (MQ=255)

               aaTGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGc                                                                                                             >  2:584036/1‑140 (MQ=255)

                    gtgtgtttAATCCCCTTCCCCACAGCCAATACCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGca                                                                                                        <  2:1037803/140‑1 (MQ=255)

                       tgtgtAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGcatca                                                                                                     >  2:587156/1‑140 (MQ=255)

                               ccccTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCcgcg                                                                                             <  1:705521/139‑1 (MQ=255)

                               ccccTTCACCACAGCCAATACCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCcgcg                                                                                             <  1:280313/139‑1 (MQ=255)

                                cccTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCgcgc                                                                                            >  1:909538/1‑140 (MQ=255)

                                 ccTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCt                                                                                           <  2:204493/140‑1 (MQ=255)

                                 ccTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCt                                                                                           <  1:460305/140‑1 (MQ=255)

                                   ttCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGt                                                                                         >  2:997829/1‑140 (MQ=255)

                                   ttCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGt                                                                                         >  1:193893/1‑140 (MQ=255)

                                     caccacAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCg                                                                                       >  1:149442/1‑140 (MQ=255)

                                     caccacAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCg                                                                                       >  2:594360/1‑140 (MQ=255)

                                       ccacAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGcc                                                                                     <  2:463606/140‑1 (MQ=255)

                                         acaGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTCGCATCATTTCCGCGCTGTCGCCCg                                                                                   <  1:346203/140‑1 (MQ=255)

                                          caGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCgg                                                                                  >  1:945195/1‑140 (MQ=255)

                                           aGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGa                                                                                 >  1:872748/1‑140 (MQ=255)

                                           aGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTACGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGa                                                                                 <  1:777320/140‑1 (MQ=255)

                                           aGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCATTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGa                                                                                 >  1:745614/1‑140 (MQ=255)

                                                  atCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACgag                                                                          <  2:303072/140‑1 (MQ=255)

                                                       gCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGt                                                                     >  2:225376/1‑140 (MQ=255)

                                                       gCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGt                                                                     >  2:350302/1‑140 (MQ=255)

                                                             ccgTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGc                                                               >  1:9392/1‑140 (MQ=255)

                                                                  gCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAAc                                                          <  2:179484/140‑1 (MQ=255)

                                                                      tATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGgc                                                      <  1:466833/140‑1 (MQ=255)

                                                                      tATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGgc                                                      <  1:1031753/140‑1 (MQ=255)

                                                                       atcatcAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGgca                                                     >  1:616929/1‑140 (MQ=255)

                                                                        tcatcaAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGgcag                                                    <  1:997829/140‑1 (MQ=255)

                                                                            caAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGa                                                <  1:796119/140‑1 (MQ=255)

                                                                             aaGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGAt                                               >  1:826308/1‑140 (MQ=255)

                                                                              aGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGAtt                                              >  1:156107/1‑140 (MQ=255)

                                                                                ccGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGa                                            <  1:243951/140‑1 (MQ=255)

                                                                                      ccTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATAcc                                      <  1:1003015/140‑1 (MQ=255)

                                                                                               ggCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCg                             <  1:587156/140‑1 (MQ=255)

                                                                                               ggCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCg                             <  1:627448/140‑1 (MQ=255)

                                                                                               ggCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCg                             >  2:805088/1‑140 (MQ=255)

                                                                                               ggCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCg                             <  2:890903/140‑1 (MQ=255)

                                                                                                    cAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCata                        >  1:436348/1‑140 (MQ=255)

                                                                                                      aTTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATc                      >  1:597013/1‑140 (MQ=255)

                                                                                                         gCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCaaa                   <  2:616929/140‑1 (MQ=255)

                                                                                                               cGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATg             >  1:602871/1‑140 (MQ=255)

                                                                                                                  aGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCCTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTCACAAACCCTTCTTTCGCCATATCAAACTGATGTCg          >  2:277649/1‑140 (MQ=255)

                                                                                                                   gCGGCTGATAATGTCCGACTTCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGc         <  2:888353/140‑1 (MQ=255)

                                                                                                                   gCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGc         <  2:297905/140‑1 (MQ=255)

                                                                                                                    cGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCt        <  1:1036653/140‑1 (MQ=255)

                                                                                                                    cGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCt        <  2:945195/140‑1 (MQ=255)

                                                                                                                    cGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCt        <  1:594360/140‑1 (MQ=255)

                                                                                                                     ggCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTg       <  1:961030/140‑1 (MQ=255)

                                                                                                                          aTAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGAc  <  1:350302/140‑1 (MQ=255)

GGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGAC  >  NC_000913/1906738‑1906999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A