Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,269	A→G	P264P (CCA→CCG)	msrP →	periplasmic protein‑L‑methionine sulfoxide reductase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,269	0	A	G	100.0%	134.4 / NA	36	P264P (CCA→CCG)	msrP	periplasmic protein‑L‑methionine sulfoxide reductase catalytic subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/18); total (18/18)

ATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGA  >  NC_000913/2040135‑2040391

atgaCCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGaccac                                                                                                                       >  1:61741/1‑140 (MQ=255)

atgaCCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGaccac                                                                                                                       >  1:979798/1‑140 (MQ=255)

 ggaCGGTGGGTGTTTAGGGCAGGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGaccacc                                                                                                                      <  2:730702/139‑1 (MQ=255)

 ggaCCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGaccacc                                                                                                                      <  1:388576/139‑1 (MQ=255)

    ccGTAGGGGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTgg                                                                                                                   >  1:716810/1‑140 (MQ=255)

     cGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGa                                                                                                                  >  1:117605/1‑140 (MQ=255)

        aGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATc                                                                                                               >  2:268998/1‑140 (MQ=255)

        aGGTGTTTATGGCAAGGCGTAACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATc                                                                                                               >  2:655359/1‑140 (MQ=255)

               tATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAgc                                                                                                        >  1:669948/1‑140 (MQ=255)

                    cAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTg                                                                                                   <  2:465647/140‑1 (MQ=255)

                       ggCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACg                                                                                                >  2:1013461/1‑140 (MQ=255)

                            tACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATAc                                                                                           <  2:381706/140‑1 (MQ=255)

                                       aaTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc                                                                                <  2:140265/140‑1 (MQ=255)

                                       aaTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc                                                                                <  2:875389/140‑1 (MQ=255)

                                       aaTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc                                                                                <  2:302360/140‑1 (MQ=255)

                                       aaTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc                                                                                <  1:268998/140‑1 (MQ=255)

                                        aTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGcc                                                                               <  2:300974/140‑1 (MQ=255)

                                         tGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCa                                                                              <  1:252570/140‑1 (MQ=255)

                                              cgcCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGtt                                                                         <  1:910334/140‑1 (MQ=255)

                                                 cGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAAt                                                                      <  1:49573/140‑1 (MQ=255)

                                                                tgCCGTTGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCAc                                                       >  2:211810/1‑140 (MQ=255)

                                                                 gCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTGTGTTGATCAcc                                                      <  1:735154/140‑1 (MQ=255)

                                                                 gCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCAcc                                                      <  2:48265/140‑1 (MQ=255)

                                                                     tGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCcgcg                                                  <  2:61741/140‑1 (MQ=255)

                                                                       gAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCt                                                >  2:931921/1‑140 (MQ=255)

                                                                       gAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCt                                                >  2:642713/1‑140 (MQ=255)

                                                                                 tttAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGc                                      >  2:70563/1‑140 (MQ=255)

                                                                                  ttAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCt                                     >  1:225313/1‑140 (MQ=255)

                                                                                        ggATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGaa                               <  1:1013461/140‑1 (MQ=255)

                                                                                                   aTAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTgg                    >  1:407990/1‑140 (MQ=255)

                                                                                                         aGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAgg              <  2:440009/140‑1 (MQ=255)

                                                                                                                aGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATc       >  2:942586/1‑140 (MQ=255)

                                                                                                                    gACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCg   <  1:655359/140‑1 (MQ=255)

                                                                                                                     aCCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa  >  2:746362/1‑140 (MQ=255)

                                                                                                                     aCCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa  >  2:246568/1‑140 (MQ=255)

                                                                                                                     aCCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa  >  1:816898/1‑140 (MQ=255)

                                                                                                                     aCCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa  >  1:820600/1‑140 (MQ=255)

ATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGA  >  NC_000913/2040135‑2040391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A