Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,297,107	T→C	I180V (ATT→GTT)	ccmA ←	heme trafficking system ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,297,107	0	T	C	97.9%	169.3 / ‑3.1	47	coding (538/624 nt)	ccmA	heme trafficking system ATP‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (18/28); minor base . (0/1); total (18/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCGG  >  NC_000913/2296970‑2297240

cgcTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAccc                                                                                                                                     >  1:872014/1‑140 (MQ=255)

      ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCt                                                                                                                               <  1:351280/140‑1 (MQ=255)

      ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCt                                                                                                                               <  2:575054/140‑1 (MQ=255)

      ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCt                                                                                                                               <  1:840263/140‑1 (MQ=255)

      ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCt                                                                                                                               <  2:251031/140‑1 (MQ=255)

       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTg                                                                                                                              <  1:752379/140‑1 (MQ=255)

           aaCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTcc                                                                                                                          <  2:270692/140‑1 (MQ=255)

               cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTAt                                                                                                                      >  1:928055/1‑140 (MQ=255)

                  cacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCCGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTAtgct                                                                                                                   <  2:773823/140‑1 (MQ=255)

                  cacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTAtgct                                                                                                                   <  2:182411/140‑1 (MQ=255)

                     gCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTgcg                                                                                                                >  2:1022241/1‑140 (MQ=255)

                      cAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTgcgc                                                                                                               <  1:380108/140‑1 (MQ=255)

                      cAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAA‑CCCCCCCTGCTCCGTATGCTGCGcc                                                                                                              <  1:972713/140‑1 (MQ=255)

                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGcc                                                                                                              >  1:569772/1‑140 (MQ=255)

                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCCGCGcc                                                                                                              >  2:41468/1‑140 (MQ=255)

                               aCGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTg                                                                                                      >  1:368972/1‑140 (MQ=255)

                                   aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc                                                                                                  >  1:634751/1‑140 (MQ=255)

                                   aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc                                                                                                  >  2:410099/1‑140 (MQ=255)

                                   aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc                                                                                                  >  1:526568/1‑140 (MQ=255)

                                   aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc                                                                                                  <  2:502054/140‑1 (MQ=255)

                                             aGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGa                                                                                        >  2:530730/1‑140 (MQ=255)

                                              gAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGac                                                                                       <  2:103399/140‑1 (MQ=255)

                                                    catGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGtt                                                                                 <  2:755118/140‑1 (MQ=255)

                                                      tGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTaa                                                                               >  2:968265/1‑140 (MQ=255)

                                                         ggCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGt                                                                            >  1:616643/1‑140 (MQ=255)

                                                           cccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCa                                                                          >  2:372977/1‑140 (MQ=255)

                                                           cccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCa                                                                          >  1:97043/1‑140 (MQ=255)

                                                            ccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCaa                                                                         >  2:106045/1‑140 (MQ=255)

                                                            ccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCaa                                                                         <  1:1022241/140‑1 (MQ=255)

                                                              tCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATc                                                                       >  2:48743/1‑140 (MQ=255)

                                                               cGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcg                                                                      <  2:926284/140‑1 (MQ=255)

                                                                       tCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTaaaa                                                              <  2:216010/140‑1 (MQ=255)

                                                                       tCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTaaaa                                                              <  2:569772/140‑1 (MQ=255)

                                                                         aGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAgg                                                            <  2:634751/140‑1 (MQ=255)

                                                                           tGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCt                                                          <  1:530730/140‑1 (MQ=255)

                                                                                tGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                     >  2:284265/1‑140 (MQ=255)

                                                                                tGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                     <  2:981044/140‑1 (MQ=255)

                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                                                    <  1:200840/140‑1 (MQ=255)

                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                                                    <  2:368972/140‑1 (MQ=255)

                                                                                  cggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg                                                   >  2:497248/1‑140 (MQ=255)

                                                                                  cggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg                                                   <  2:249032/140‑1 (MQ=255)

                                                                                       aaTTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATcc                                              >  2:444465/1‑140 (MQ=255)

                                                                                            tACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAAGCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAAc                                         <  2:97043/140‑1 (MQ=255)

                                                                                                                 gCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGa                    <  1:48743/140‑1 (MQ=255)

                                                                                                                   tggtggGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGTGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGAcg                  <  2:941039/140‑1 (MQ=255)

                                                                                                                     gtggGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGAcgcg                <  1:41468/140‑1 (MQ=255)

                                                                                                                                gAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGAcg     <  1:667142/140‑1 (MQ=255)

                                                                                                                                   tCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACgcgg  <  2:526568/140‑1 (MQ=255)

CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCGG  >  NC_000913/2296970‑2297240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A