Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,745,777	A→G	F48L (TTT→CTT)	rimM ←	ribosome maturation factor RimM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,745,777	0	A	G	100.0%	188.8 / NA	51	F48L (TTT→CTT)	rimM	ribosome maturation factor RimM
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/24); total (27/24)

TTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTGCGCGGTGAGTTG  >  NC_000913/2745656‑2745909

ttCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCaaa                                                                                                                    <  1:540865/140‑1 (MQ=255)

 tCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCaaaa                                                                                                                   <  1:504676/140‑1 (MQ=255)

  cGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCaaaaa                                                                                                                  >  1:516968/1‑140 (MQ=255)

          ggTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCg                                                                                                          <  2:913253/140‑1 (MQ=255)

           gTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCgg                                                                                                         >  1:275329/1‑140 (MQ=255)

              cgccgcATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGt                                                                                                      >  1:276997/1‑140 (MQ=255)

                ccgcATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCt                                                                                                    >  1:502876/1‑140 (MQ=255)

                   cATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGTTGATCGTCAAAAATGCTTTCGGCGTCTTCg                                                                                                 <  1:923833/140‑1 (MQ=255)

                    aTCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCgg                                                                                                <  1:214080/140‑1 (MQ=255)

                    aTCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCgg                                                                                                <  2:499398/140‑1 (MQ=255)

                      cACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTg                                                                                              <  1:686029/140‑1 (MQ=255)

                              tCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGCAGaaaa                                                                                      <  1:311352/140‑1 (MQ=255)

                              tCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGaaaa                                                                                      >  2:897926/1‑140 (MQ=255)

                                aaCGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAaca                                                                                    >  1:58783/1‑140 (MQ=255)

                                   gCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTACCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACActc                                                                                 <  1:250211/140‑1 (MQ=255)

                                     cTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTg                                                                               >  2:887898/1‑140 (MQ=255)

                                       ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             <  2:468484/140‑1 (MQ=255)

                                       ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             >  2:386347/1‑140 (MQ=255)

                                       ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             <  2:1011506/140‑1 (MQ=255)

                                       ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             >  1:517917/1‑140 (MQ=255)

                                       ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             <  1:450244/140‑1 (MQ=255)

                                        tCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGc                                                                            <  2:523525/140‑1 (MQ=255)

                                          aGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGcca                                                                          <  2:570880/140‑1 (MQ=255)

                                              tgatgaTCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGccaccca                                                                      <  2:915719/140‑1 (MQ=255)

                                                atgatCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACg                                                                    <  1:235926/140‑1 (MQ=255)

                                                  gatCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGaa                                                                  <  1:452023/140‑1 (MQ=255)

                                                      aTGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATAcc                                                              >  2:686110/1‑140 (MQ=255)

                                                          ccTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTaa                                                          >  2:697620/1‑140 (MQ=255)

                                                          ccTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTaa                                                          >  1:683163/1‑140 (MQ=255)

                                                              aTTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACg                                                      >  2:393741/1‑140 (MQ=255)

                                                                tgtgGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGaa                                                    <  2:58783/140‑1 (MQ=255)

                                                                 gtggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAc                                                   >  1:936848/1‑140 (MQ=255)

                                                                 gtggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAc                                                   <  2:258082/140‑1 (MQ=255)

                                                                   ggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAccc                                                 >  2:930623/1‑140 (MQ=255)

                                                                   ggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAccc                                                 >  1:10768/1‑140 (MQ=255)

                                                                   ggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAccc                                                 >  2:213041/1‑140 (MQ=255)

                                                                        ttccagctttccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCAtttt                                            >  1:733046/1‑140 (MQ=255)

                                                                        ttccagctttccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCAtttt                                            >  1:396124/1‑140 (MQ=255)

                                                                          ccagctttccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTc                                          >  2:169812/1‑140 (MQ=255)

                                                                                 tccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAAc                                   >  1:362356/1‑140 (MQ=255)

                                                                                  ccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACg                                  <  2:159629/140‑1 (MQ=255)

                                                                                            ttGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCaa                        <  1:534942/140‑1 (MQ=255)

                                                                                             tgctgcCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAAc                       <  2:51758/140‑1 (MQ=255)

                                                                                              gctgcCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACa                      >  1:250730/1‑140 (MQ=255)

                                                                                               ctgcCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAg                     >  1:104097/1‑140 (MQ=255)

                                                                                                   cACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGc                 >  1:470778/1‑140 (MQ=255)

                                                                                                       gACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTgt             <  2:516968/140‑2 (MQ=255)

                                                                                                        aCCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTgcg            >  2:516703/1‑140 (MQ=255)

                                                                                                        aCCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTgcg            >  2:662003/1‑140 (MQ=255)

                                                                                                              cTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTGCGCGGTGa      <  1:697620/140‑1 (MQ=255)

                                                                                                                  tGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTGCGCGGTGAgttg  >  1:303833/1‑140 (MQ=255)

TTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTGCGCGGTGAGTTG  >  NC_000913/2745656‑2745909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A