Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,749,090	T→C	L106P (CTG→CCG)	yfjD →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,749,090	0	T	C	100.0%	191.3 / NA	53	L106P (CTG→CCG)	yfjD	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/26); total (27/26)

CGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCACCCGT  >  NC_000913/2748954‑2749226

cGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGt                                                                                                                                       <  2:171154/140‑1 (MQ=255)

  ccTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAt                                                                                                                                     >  1:163437/1‑140 (MQ=255)

  ccTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAt                                                                                                                                     <  2:513811/140‑1 (MQ=255)

  ccTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAt                                                                                                                                     >  2:854130/1‑140 (MQ=255)

   cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAtt                                                                                                                                    <  2:1019105/140‑1 (MQ=255)

   cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAtt                                                                                                                                    >  2:908755/1‑140 (MQ=255)

      aTAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGc                                                                                                                                 <  1:908755/140‑1 (MQ=255)

               gtgtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTAtt                                                                                                                        >  1:834975/1‑140 (MQ=255)

                tgtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTg                                                                                                                       >  1:1052008/1‑140 (MQ=255)

                tgtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTg                                                                                                                       >  2:396872/1‑140 (MQ=255)

                    aaTCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGa                                                                                                                   >  1:920051/1‑140 (MQ=255)

                          cAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGTGGTATTGCCGAAAACCa                                                                                                             >  2:327617/1‑140 (MQ=255)

                          cAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCa                                                                                                             <  1:214247/140‑1 (MQ=255)

                          cAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCa                                                                                                             >  1:3765/1‑140 (MQ=255)

                              aaCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGc                                                                                                         <  2:834975/140‑1 (MQ=255)

                                  tGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCcgcg                                                                                                     <  1:396872/140‑1 (MQ=255)

                                  tGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCcgcg                                                                                                     <  2:3765/140‑1 (MQ=255)

                                    gTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCt                                                                                                   <  1:482052/140‑1 (MQ=255)

                                    gTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCt                                                                                                   >  1:258391/1‑140 (MQ=255)

                                    gTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCt                                                                                                   <  1:101220/140‑1 (MQ=255)

                                        atatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTAc                                                                                               <  1:837713/140‑1 (MQ=255)

                                              ttGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaa                                                                                         >  1:512153/1‑140 (MQ=255)

                                              ttGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaa                                                                                         >  1:146021/1‑140 (MQ=255)

                                                     cgcgCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg                                                                                  <  2:568508/140‑1 (MQ=255)

                                                     cgcgCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg                                                                                  <  2:920051/140‑1 (MQ=255)

                                                          tCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTAt                                                                             <  1:747716/140‑1 (MQ=255)

                                                                           aTGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTctgct                                                            >  1:17876/1‑140 (MQ=255)

                                                                             gCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTgg                                                          >  2:431891/1‑140 (MQ=255)

                                                                                    tACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCt                                                   >  2:174613/1‑140 (MQ=255)

                                                                                     aCGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTg                                                  <  2:512153/140‑1 (MQ=255)

                                                                                      cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAGCCATTGTCGCGATGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTACTGGCTCCGCTGc                                                 <  2:803588/140‑1 (MQ=255)

                                                                                        gCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGTAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCa                                                <  2:964719/139‑1 (MQ=255)

                                                                                        gCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCaa                                               >  2:808920/1‑140 (MQ=255)

                                                                                        gCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCa                                                >  1:964719/1‑139 (MQ=255)

                                                                                           aTGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAAtt                                            >  2:142017/1‑140 (MQ=255)

                                                                                           aTGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAAtt                                            >  1:723764/1‑140 (MQ=255)

                                                                                           aTGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAAtt                                            >  2:511644/1‑140 (MQ=255)

                                                                                           aTGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAAtt                                            >  2:520254/1‑140 (MQ=255)

                                                                                                      gCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGcc                                 >  2:578916/1‑140 (MQ=255)

                                                                                                             cGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGctggtc                          <  1:142017/140‑1 (MQ=255)

                                                                                                                cTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGTGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGctggtctgg                       <  1:327617/140‑1 (MQ=255)

                                                                                                                cTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGctggtctgg                       <  1:174613/140‑1 (MQ=255)

                                                                                                                    tgtgCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGc                   >  2:561974/1‑140 (MQ=255)

                                                                                                                        cTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGaa               >  2:616282/1‑140 (MQ=255)

                                                                                                                             ttttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTa          >  2:621047/1‑140 (MQ=255)

                                                                                                                              tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         <  2:1052008/140‑1 (MQ=255)

                                                                                                                              tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         <  2:723764/140‑1 (MQ=255)

                                                                                                                              tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         <  2:146021/140‑1 (MQ=255)

                                                                                                                              tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         <  1:431891/140‑1 (MQ=255)

                                                                                                                              tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         >  1:250774/1‑140 (MQ=255)

                                                                                                                                 gTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCAc      <  2:184705/140‑1 (MQ=255)

                                                                                                                                   cGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCAccc    <  1:640434/140‑1 (MQ=255)

                                                                                                                                     tACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTg                    <  2:101656/122‑1 (MQ=255)

                                                                                                                                     tACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCACCCGt  >  2:485723/1‑140 (MQ=255)

CGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCACCCGT  >  NC_000913/2748954‑2749226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A