Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,763,764	A→G	F464L (TTT→CTT)	abpA ←	CP4‑57 prophage; anti‑bacteriophage protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,763,764	0	A	G	100.0%	135.0 / NA	36	F464L (TTT→CTT)	abpA	CP4‑57 prophage; anti‑bacteriophage protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/23); total (13/23)

ACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTC  >  NC_000913/2763632‑2763899

aCGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGaaa                                                                                                                                  >  2:857683/1‑140 (MQ=255)

  ggCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACg                                                                                                                                >  2:410222/1‑140 (MQ=255)

            cGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGa                                                                                                                      <  1:1022784/140‑1 (MQ=255)

             gATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAg                                                                                                                     <  1:375825/140‑1 (MQ=255)

               tGAGGTCAGCCGCGAATTCTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCa                                                                                                                   <  1:16155/140‑1 (MQ=255)

                           cgAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTgg                                                                                                       <  1:236855/140‑1 (MQ=255)

                             aaTTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCt                                                                                                     >  1:103329/1‑140 (MQ=255)

                             aaTTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCt                                                                                                     >  2:317488/1‑140 (MQ=255)

                             aaTTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCt                                                                                                     >  1:535673/1‑140 (MQ=255)

                              aTTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTg                                                                                                    <  1:875289/140‑1 (MQ=255)

                                ttttCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGcc                                                                                                  <  1:631869/140‑1 (MQ=255)

                                     gAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCaa                                                                                             <  2:485689/140‑1 (MQ=255)

                                      aaCAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAg                                                                                            <  1:731668/140‑1 (MQ=255)

                                         aGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAAt                                                                                         <  2:465719/140‑1 (MQ=255)

                                                cTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCa                                                                                  <  2:226409/140‑1 (MQ=255)

                                                 tGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCaa                                                                                 <  1:368010/140‑1 (MQ=255)

                                                    ttctttctCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGt                                                                              <  2:566436/140‑1 (MQ=255)

                                                         tctcCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTgg                                                                         <  1:857683/140‑1 (MQ=255)

                                                              aGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAg                                                                    <  1:723871/140‑1 (MQ=255)

                                                                          aaCCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATc                                                        <  2:103329/140‑1 (MQ=255)

                                                                          aaCCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATc                                                        <  2:403542/140‑1 (MQ=255)

                                                                                   gTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAAt                                               >  1:342049/1‑140 (MQ=255)

                                                                                              ggTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttt                                    >  1:924205/1‑140 (MQ=255)

                                                                                                ttAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttttt                                  <  2:638804/140‑1 (MQ=255)

                                                                                                     agATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcg                             <  1:552742/140‑1 (MQ=255)

                                                                                                      gATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgc                            <  2:535673/140‑1 (MQ=255)

                                                                                                       aTTAGATTCGTATCTCACGAGCAGAACAAGTGTTAAACGATCGATGTGAGCTTCGAAAGGTTTGCTGCCATCAATAATTTCGTCAATTTCGTTGCGAAGCAGGTATTTATCGTCTGTAATGTCGAGGATTTTTTTgcgcg                           <  2:218325/140‑1 (MQ=255)

                                                                                                       aTTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgcg                           <  2:286926/140‑1 (MQ=255)

                                                                                                        ttAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgcgc                          >  1:79614/1‑140 (MQ=255)

                                                                                                              tCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgcg                           >  1:769065/1‑133 (MQ=255)

                                                                                                              tCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgcg                           <  2:769065/133‑1 (MQ=255)

                                                                                                                 tAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGAt                 >  1:371458/1‑140 (MQ=255)

                                                                                                                 tAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGAt                 >  1:474538/1‑140 (MQ=255)

                                                                                                                   gCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATTGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGc               <  1:437074/140‑1 (MQ=255)

                                                                                                                               aacaAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGt   <  2:371458/140‑1 (MQ=255)

                                                                                                                               aacaAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGt   >  1:668162/1‑140 (MQ=255)

                                                                                                                                acaAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTc  >  1:1018336/1‑140 (MQ=255)

ACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTC  >  NC_000913/2763632‑2763899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A