Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,080,055	T→C	E527E (GAA→GAG)	tktA ←	transketolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,080,055	0	T	C	100.0%	177.6 / NA	48	E527E (GAA→GAG)	tktA	transketolase 1
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/32); total (16/32)

CAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCA  >  NC_000913/3079919‑3080191

cagtcagtTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTcc                                                                                                                                       <  2:1019467/140‑1 (MQ=255)

   tcagtTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCctgc                                                                                                                                    <  1:71224/140‑1 (MQ=255)

     agtTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCctgctg                                                                                                                                  <  1:492021/140‑1 (MQ=255)

        ttttCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTgcgc                                                                                                                               <  1:261059/140‑1 (MQ=255)

         tttCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGcc                                                                                                                              <  1:849819/140‑1 (MQ=255)

         tttCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGcc                                                                                                                              <  2:896730/140‑1 (MQ=255)

              tAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGGGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGtt                                                                                                                         <  2:788043/140‑1 (MQ=255)

                ggCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCt                                                                                                                       <  1:254804/140‑1 (MQ=255)

                ggCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCt                                                                                                                       <  2:329876/140‑1 (MQ=255)

                 gcagcaACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTg                                                                                                                      >  2:345324/1‑140 (MQ=255)

                  cagcaACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGa                                                                                                                     <  2:866186/140‑1 (MQ=255)

                  cagcaACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGa                                                                                                                     <  2:47476/140‑1 (MQ=255)

                   agcaACAGGCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCTGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGAc                                                                                                                    <  2:227136/140‑1 (MQ=255)

                   agcaACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGAc                                                                                                                    <  1:400819/140‑1 (MQ=255)

                     caACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACgg                                                                                                                  <  1:140132/140‑1 (MQ=255)

                     caACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACgg                                                                                                                  >  1:151439/1‑140 (MQ=255)

                           ccAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAgg                                                                                                            <  2:1011548/140‑1 (MQ=255)

                           ccAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGATGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTg                                                                                                                      >  2:937209/1‑130 (MQ=255)

                           ccAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGATGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTg                                                                                                                      <  1:937209/130‑1 (MQ=255)

                                          aaCCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCGGTGCGGTCggg                                                                                             >  2:435298/1‑140 (MQ=255)

                                               gTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTc                                                                                        >  2:1030255/1‑140 (MQ=255)

                                                     aTGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACg                                                                                  <  1:849499/140‑1 (MQ=255)

                                                           aTCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAac                                                                            <  2:859361/140‑1 (MQ=255)

                                                            tCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAaca                                                                           <  2:151439/140‑1 (MQ=255)

                                                                   cGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCGCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTAtt                                                                    >  2:774221/1‑140 (MQ=255)

                                                                     gCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTc                                                                  >  2:965675/1‑140 (MQ=255)

                                                                         aCCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcg                                                              <  2:91575/140‑1 (MQ=255)

                                                                                    cTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGAt                                                   <  2:140082/140‑1 (MQ=255)

                                                                                     tttCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGAtt                                                  <  2:145650/140‑1 (MQ=255)

                                                                                              aTAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGt                                         <  1:220213/140‑1 (MQ=255)

                                                                                               tAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGGTCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTc                                        >  2:581642/1‑140 (MQ=255)

                                                                                                   caccGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCacac                                    <  1:463151/140‑1 (MQ=255)

                                                                                                    accGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACg                                   <  1:477834/140‑1 (MQ=255)

                                                                                                     ccGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACgg                                  >  1:542580/1‑140 (MQ=255)

                                                                                                        cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               >  1:676452/1‑140 (MQ=255)

                                                                                                        cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               >  2:856758/1‑140 (MQ=255)

                                                                                                        cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               <  1:861619/140‑1 (MQ=255)

                                                                                                        cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               >  1:368705/1‑140 (MQ=255)

                                                                                                        cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               >  1:212685/1‑140 (MQ=255)

                                                                                                                  ttGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                     <  1:345324/140‑1 (MQ=255)

                                                                                                                  ttGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                     <  2:358951/140‑1 (MQ=255)

                                                                                                                  ttGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                     <  2:264712/140‑1 (MQ=255)

                                                                                                                  ttGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                     <  1:1030255/140‑1 (MQ=255)

                                                                                                                   tGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACa                    >  1:692984/1‑140 (MQ=255)

                                                                                                                       aGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGtt                <  1:446923/140‑1 (MQ=255)

                                                                                                                       aGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGtt                <  2:218389/140‑1 (MQ=255)

                                                                                                                         ttGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCg              >  2:267519/1‑140 (MQ=255)

                                                                                                                           gCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg            >  2:947190/1‑140 (MQ=255)

                                                                                                                                   gttcgctcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTAcgcg    >  2:585605/1‑140 (MQ=255)

                                                                                                                                   gttcgctcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTAcgcg    >  2:456668/1‑140 (MQ=255)

                                                                                                                                     tcgctcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCa  <  1:976369/140‑1 (MQ=255)

                                                                                                                                     tcgctcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCa  <  1:856758/140‑1 (MQ=255)

CAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCA  >  NC_000913/3079919‑3080191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A