Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,080,867	T→C	T257A (ACC→GCC)	tktA ←	transketolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,080,867	0	T	C	100.0%	178.3 / NA	49	T257A (ACC→GCC)	tktA	transketolase 1
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/18); total (31/18)

CCTGGCCTGCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCG  >  NC_000913/3080732‑3080996

cctggcctgCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCg                                                                                                                               >  2:456899/1‑140 (MQ=255)

    gcctgCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCtt                                                                                                                           >  1:336252/1‑140 (MQ=255)

    gcctgCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCtt                                                                                                                           >  1:863986/1‑140 (MQ=255)

     cctgCTTCTTTCGCATCCCACTGAGCATAGCTTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGCCGCCCAGCGGCGCACCGTGGGAGCCGTGGGCACCGGCttt                                                                                                                          >  2:202739/1‑140 (MQ=255)

     cctgCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCttt                                                                                                                          >  2:958580/1‑140 (MQ=255)

              ttCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTCCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGa                                                                                                                 >  1:1061335/1‑140 (MQ=255)

                   aTCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGaaccga                                                                                                            <  2:863986/140‑1 (MQ=255)

                             gCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCgatgat                                                                                                  >  1:563801/1‑140 (MQ=255)

                               aTAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATgg                                                                                                >  2:969504/1‑140 (MQ=255)

                               aTAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATgg                                                                                                >  1:740713/1‑140 (MQ=255)

                                 aGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGtt                                                                                              >  1:771383/1‑140 (MQ=255)

                                  gATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGttt                                                                                             >  1:238740/1‑140 (MQ=255)

                                      tCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTAAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGca                                                                                         >  2:1094901/1‑140 (MQ=255)

                                        aGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGcaca                                                                                       >  1:935271/1‑140 (MQ=255)

                                            gggATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcag                                                                                   >  1:688626/1‑140 (MQ=255)

                                            gggATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTCCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcag                                                                                   <  1:742323/140‑1 (MQ=255)

                                              gATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcagca                                                                                 <  2:238740/140‑1 (MQ=255)

                                              gATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcagca                                                                                 >  2:247507/1‑140 (MQ=255)

                                              gATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcagca                                                                                 <  2:336252/140‑1 (MQ=255)

                                                    gAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAg                                                                           >  1:885565/1‑140 (MQ=255)

                                                    gAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAg                                                                           <  1:969504/140‑1 (MQ=255)

                                                    gAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAg                                                                           <  1:113716/140‑1 (MQ=255)

                                                        ggCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGttt                                                                       >  2:203138/1‑140 (MQ=255)

                                                        ggCGCATATTTCCAGCCCAGTTGTCCGCGGGTCAGGGCAA‑TTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTg                                                                      >  1:908891/1‑140 (MQ=255)

                                                          cgcATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGt                                                                         >  1:422019/1‑136 (MQ=255)

                                                          cgcATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGt                                                                         <  2:422019/136‑1 (MQ=255)

                                                            cATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTgtca                                                                   >  1:414673/1‑140 (MQ=255)

                                                              tatTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTgtcagt                                                                 <  2:414673/140‑1 (MQ=255)

                                                                    cAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgc                                                           >  1:192083/1‑140 (MQ=255)

                                                                     aGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcg                                                          <  2:740713/140‑1 (MQ=255)

                                                                       cccAGTTGTCCGCAGGTCAGGGCAA‑TTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgc                                                       <  2:908891/140‑1 (MQ=255)

                                                                        ccAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgc                                                       <  2:192083/140‑1 (MQ=255)

                                                                        ccAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgc                                                       <  2:885565/140‑1 (MQ=255)

                                                                           gttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGct                                                    >  2:1126897/1‑140 (MQ=255)

                                                                           gttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGct                                                    >  1:531926/1‑140 (MQ=255)

                                                                            ttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGctt                                                   >  2:396139/1‑140 (MQ=255)

                                                                            ttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGctt                                                   >  1:1032499/1‑140 (MQ=255)

                                                                              gttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGcttct                                                 <  1:203138/140‑1 (MQ=255)

                                                                                 cgcgGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTtcttct                                              >  2:1164706/1‑140 (MQ=255)

                                                                                      gTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgcgc                                                     <  2:789552/128‑1 (MQ=255)

                                                                                      gTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgcgc                                                     >  1:789552/1‑128 (MQ=255)

                                                                                                tttCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATa                               >  2:931379/1‑140 (MQ=255)

                                                                                                            ccAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCa                   <  1:958580/140‑1 (MQ=255)

                                                                                                                cggcgCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGAc               >  2:1168516/1‑140 (MQ=255)

                                                                                                                   cgcACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGt            >  1:603832/1‑140 (MQ=255)

                                                                                                                     cACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCg          >  2:1045369/1‑140 (MQ=255)

                                                                                                                     cACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCg          >  1:1018022/1‑140 (MQ=255)

                                                                                                                     cACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCg          >  1:401544/1‑140 (MQ=255)

                                                                                                                       ccGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGAt        <  1:202739/140‑1 (MQ=255)

                                                                                                                       ccGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGAt        <  1:1126897/140‑1 (MQ=255)

                                                                                                                                    gggCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTcgcg  <  1:46636/133‑1 (MQ=255)

CCTGGCCTGCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCG  >  NC_000913/3080732‑3080996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A