Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,483,979	A→G	S260G (AGC→GGC)	yheT →	putative hydrolase YheT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,483,979	0	A	G	100.0%	200.4 / NA	54	S260G (AGC→GGC)	yheT	putative hydrolase YheT
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/28); total (26/28)

CTGGCAGCCTACCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACA  >  NC_000913/3483844‑3484115

cTGGCAGCCTTCCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGgcgc                                                                                                                                      <  1:394200/140‑1 (MQ=255)

     aGCCTACCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGc                                                                                                                                 <  2:858573/140‑1 (MQ=255)

      gCCTACCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTGCGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGcc                                                                                                                                <  1:305916/140‑1 (MQ=255)

          cccccGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATg                                                                                                                            <  2:333615/139‑1 (MQ=255)

                 aaCGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAAcc                                                                                                                     >  2:840126/1‑140 (MQ=255)

                     cTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGAt                                                                                                                 >  2:828151/1‑140 (MQ=255)

                      tGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATc                                                                                                                >  2:37530/1‑140 (MQ=255)

                      tGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATc                                                                                                                >  2:488662/1‑140 (MQ=255)

                             tAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATGACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAAc                                                                                                         <  1:633376/140‑1 (MQ=255)

                             tAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAAc                                                                                                         <  1:391558/140‑1 (MQ=255)

                                     cgcAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTg                                                                                                 >  1:325059/1‑140 (MQ=255)

                                           tAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGattatt                                                                                           <  2:218327/140‑1 (MQ=255)

                                                  ggTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCa                                                                                    <  1:781710/140‑1 (MQ=255)

                                                    tACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCaaa                                                                                  >  1:460395/1‑140 (MQ=255)

                                                    tACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCaaa                                                                                  >  1:467731/1‑140 (MQ=255)

                                                      cGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGa                                                                                <  1:449752/140‑1 (MQ=255)

                                                      cGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGa                                                                                <  1:342301/140‑1 (MQ=255)

                                                         cGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGa                                                                             <  1:433616/140‑1 (MQ=255)

                                                          gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGAt                                                                            <  1:488662/140‑1 (MQ=255)

                                                          gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGAt                                                                            <  1:621990/140‑1 (MQ=255)

                                                          gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGAt                                                                            <  1:776845/140‑1 (MQ=255)

                                                              ccGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGt                                                                        >  2:983163/1‑140 (MQ=255)

                                                                gTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGttt                                                                      >  2:232499/1‑140 (MQ=255)

                                                                  gAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTAt                                                                    <  1:37530/140‑1 (MQ=255)

                                                                    aTTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATgg                                                                  <  1:840126/140‑1 (MQ=255)

                                                                      ttGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGat                                                                >  1:900789/1‑140 (MQ=255)

                                                                        gACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGatca                                                              >  1:950388/1‑140 (MQ=255)

                                                                              ctcATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGt                                                        >  1:703401/1‑140 (MQ=255)

                                                                              ctcATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGt                                                        >  2:868631/1‑140 (MQ=255)

                                                                                        ccAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGaaa                                              >  2:348525/1‑140 (MQ=255)

                                                                                        ccAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGaaa                                              >  2:973039/1‑140 (MQ=255)

                                                                                        ccAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGaaa                                              >  1:322189/1‑140 (MQ=255)

                                                                                         cAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAAc                                             >  2:996365/1‑140 (MQ=255)

                                                                                           gAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCg                                           >  1:813251/1‑140 (MQ=255)

                                                                                           gAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCg                                           >  2:168046/1‑140 (MQ=255)

                                                                                           gAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACACCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCTAAACCg                                           >  2:751103/1‑140 (MQ=255)

                                                                                             aTTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGa                                         <  1:621089/140‑1 (MQ=255)

                                                                                                  cGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGtc                                    <  1:348525/140‑1 (MQ=255)

                                                                                                      tACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTccc                                <  1:746827/140‑1 (MQ=255)

                                                                                                        cGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTccccc                              >  1:174189/1‑140 (MQ=255)

                                                                                                             aCGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAg                         >  2:852820/1‑140 (MQ=255)

                                                                                                               gCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                       <  1:38543/140‑1 (MQ=255)

                                                                                                               gCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                       <  1:393253/140‑1 (MQ=255)

                                                                                                               gCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                       <  1:983163/140‑1 (MQ=255)

                                                                                                               gCTATCGACTATTATAGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                       <  1:389798/140‑1 (MQ=255)

                                                                                                                cTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTg                      <  1:828151/140‑1 (MQ=255)

                                                                                                                   tCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGACAGTCTCCCCCCGCAGGCGGAg                   >  2:43659/1‑140 (MQ=255)

                                                                                                                      aCTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTAt                <  2:703401/140‑1 (MQ=255)

                                                                                                                        tattatCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCa              >  2:791732/1‑140 (MQ=255)

                                                                                                                        tattatCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCa              <  1:716923/140‑1 (MQ=255)

                                                                                                                         attatCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCaa             <  1:868631/140‑1 (MQ=255)

                                                                                                                          ttatCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAac            <  2:325059/140‑1 (MQ=255)

                                                                                                                             tCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCACAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAactga         >  1:124331/1‑140 (MQ=255)

                                                                                                                                  aGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGaa    >  1:327679/1‑140 (MQ=255)

                                                                                                                                    tgtgGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACa  >  1:706592/1‑140 (MQ=255)

                                                                                                                                    tgtgGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACa  >  2:398989/1‑140 (MQ=255)

CTGGCAGCCTACCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACA  >  NC_000913/3483844‑3484115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A