Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	120	97.0	3206026	99.3%	3183583	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	711,377	A→G	V30A (GTT→GCT)	fldA ←	flavodoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	711,377	0	A	G	100.0%	223.5 / NA	59	V30A (GTT→GCT)	fldA	flavodoxin 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (38/21); total (38/21)

CTCTTCGAGAGTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAACGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATT  >  NC_000913/711241‑711511

ctctTCGAGAGTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGt                                                                                                                                     <  2:352151/140‑1 (MQ=255)

         agTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCaa                                                                                                                            >  2:266451/1‑140 (MQ=255)

          gTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAg                                                                                                                           <  2:361332/140‑1 (MQ=255)

             gggAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTg                                                                                                                        >  1:313892/1‑140 (MQ=255)

             gggAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTg                                                                                                                        >  1:259971/1‑140 (MQ=255)

             gggAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTg                                                                                                                        >  2:1505984/1‑140 (MQ=255)

                   aagTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTg                                                                                                                  <  2:686683/140‑1 (MQ=255)

                     gTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGaa                                                                                                                >  2:276587/1‑140 (MQ=255)

                                 acacTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGa                                                                                                    <  2:1247749/140‑1 (MQ=255)

                                    cTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGatat                                                                                                 >  1:1534217/1‑140 (MQ=255)

                                        gcTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTc                                                                                             <  2:83345/140‑1 (MQ=255)

                                         cTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCg                                                                                            >  2:1410190/1‑140 (MQ=255)

                                         cTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCg                                                                                            >  1:1376436/1‑140 (MQ=255)

                                            cGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTa                                                                                         >  1:661422/1‑140 (MQ=255)

                                            cGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTCCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTa                                                                                         >  1:1416663/1‑140 (MQ=255)

                                                      ccAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGgtgt                                                                               >  1:1228563/1‑140 (MQ=255)

                                                         ggTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTTTTACCGGTGTCGc                                                                            <  2:1537211/140‑1 (MQ=255)

                                                         ggTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGc                                                                            >  2:73114/1‑140 (MQ=255)

                                                         ggTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGc                                                                            >  1:259374/1‑140 (MQ=255)

                                                             gggATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGcc                                                                        >  2:136206/1‑140 (MQ=255)

                                                              ggATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCg                                                                       >  1:1459293/1‑140 (MQ=255)

                                                              ggATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCg                                                                       >  2:606421/1‑140 (MQ=255)

                                                              ggATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCg                                                                       >  1:105181/1‑140 (MQ=255)

                                                                   cccAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGaaaaa                                                                  >  1:508050/1‑140 (MQ=255)

                                                                   cccAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGaaaaa                                                                  >  1:66787/1‑140 (MQ=255)

                                                                               aTGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGAt                                                      <  2:900348/140‑1 (MQ=255)

                                                                               aTGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGAt                                                      <  1:450210/140‑1 (MQ=255)

                                                                               aTGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGAt                                                      <  2:65227/140‑1 (MQ=255)

                                                                                  tCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGAATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGc                                                   >  1:868756/1‑140 (MQ=255)

                                                                                   cATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGcc                                                  <  1:136206/140‑1 (MQ=255)

                                                                                     tAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCAt                                                >  2:1403097/1‑140 (MQ=255)

                                                                                     tAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTAGCTGCCGAAAAAGATGCCAGTGATAGCCAt                                                <  2:1584808/140‑1 (MQ=255)

                                                                                      aaGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATg                                               <  1:266451/140‑1 (MQ=255)

                                                                                       aGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGa                                              <  2:592206/140‑1 (MQ=255)

                                                                                        gCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAg                                             <  2:476115/140‑1 (MQ=255)

                                                                                        gCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAg                                             >  2:1238878/1‑140 (MQ=255)

                                                                                        gCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAg                                             <  1:276587/140‑1 (MQ=255)

                                                                                         cTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGt                                            <  1:183204/140‑1 (MQ=255)

                                                                                           tCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGa                                          >  1:1026226/1‑140 (MQ=255)

                                                                                                   ttcttTGCTGCTTTTTGCAATGTCATGGACATCGACAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACct                                  >  1:666848/1‑140 (MQ=255)

                                                                                                        tgctgcTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGa                             <  1:1403097/140‑1 (MQ=255)

                                                                                                          ctgctTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGaaa                           >  1:1419803/1‑140 (MQ=255)

                                                                                                             ctTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACtt                        >  1:1373938/1‑140 (MQ=255)

                                                                                                               ttttGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTAt                      >  1:937971/1‑140 (MQ=255)

                                                                                                               ttttGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTAt                      <  2:920903/140‑1 (MQ=255)

                                                                                                                  tGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGa                   >  2:191128/1‑140 (MQ=255)

                                                                                                                    cAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGaaa                 <  2:105181/140‑1 (MQ=255)

                                                                                                                      aTGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATg               <  2:259374/140‑1 (MQ=255)

                                                                                                                      aTGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATg               <  2:259971/140‑1 (MQ=255)

                                                                                                                         tCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATgggg            >  1:526769/1‑140 (MQ=255)

                                                                                                                          cATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATggggg           >  1:547359/1‑140 (MQ=255)

                                                                                                                          cATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATggggg           >  2:523939/1‑140 (MQ=255)

                                                                                                                            tGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATgggggcg         >  2:24935/1‑138 (MQ=255)

                                                                                                                            tGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTg         >  2:1305846/1‑140 (MQ=255)

                                                                                                                                   cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  2:933877/1‑140 (MQ=255)

                                                                                                                                   cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  2:348545/1‑140 (MQ=255)

                                                                                                                                   cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  2:1317027/1‑140 (MQ=255)

                                                                                                                                   cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  1:1263346/1‑140 (MQ=255)

                                                                                                                                   cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  1:103907/1‑140 (MQ=255)

CTCTTCGAGAGTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAACGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATT  >  NC_000913/711241‑711511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A