Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
120 |
97.0 |
3206026 |
99.3% |
3183583 |
140.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,422,015 |
A→G |
E11E (GAA→GAG) |
ydaV → |
Rac prophage; putative ATP‑binding protein YdaV |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,422,015 | 0 | A | G | 98.3%
| 214.5
/ ‑4.4
| 59 | E11E (GAA→GAG) | ydaV | Rac prophage; putative ATP‑binding protein YdaV |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (34/24); total (35/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 |
GTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGTTGCGGGTGGAAA > NC_000913/1421880‑1422151
|
gTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTa > 2:1241640/1‑140 (MQ=255)
ttCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTAt > 1:710658/1‑140 (MQ=255)
tCGCGCCGAAAGGTGTTCGCCCTGAGACGTTCCTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATc < 1:181014/140‑1 (MQ=255)
cgcCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGc > 2:994991/1‑140 (MQ=255)
cGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGa < 2:287303/140‑1 (MQ=255)
ggTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGc < 2:78566/140‑1 (MQ=255)
gtgtTCGACCTGAGACGTTCCTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGcc < 1:326986/140‑1 (MQ=255)
gtgtTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGcc < 2:87865/140‑1 (MQ=255)
tCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGt < 1:5022/140‑1 (MQ=255)
tCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGt > 1:651234/1‑140 (MQ=255)
cGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTc < 2:1167458/140‑1 (MQ=255)
gagaCGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGt > 1:654630/1‑140 (MQ=255)
tACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACcgcg < 2:1228271/140‑1 (MQ=255)
aCTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCgcgc > 1:136942/1‑140 (MQ=255)
ccccGACAGGTTAACCCCGTCTCGGTACCGGATAGTGGGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGGCTGGCCCCGTCACATGTAACCGCGcc < 2:762097/138‑1 (MQ=255)
cAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCaa < 1:1112609/140‑1 (MQ=255)
ggTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTGAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGa > 1:1386108/1‑140 (MQ=255)
ggTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGa < 2:405724/140‑1 (MQ=255)
ggTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGa < 1:994991/140‑1 (MQ=255)
ggTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGa < 1:1095963/140‑1 (MQ=255)
gTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGAc > 2:1559750/1‑140 (MQ=255)
tctcGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGGCCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAg > 1:892119/1‑140 (MQ=255)
tctcGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAg > 2:719825/1‑140 (MQ=255)
tctcGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGCAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAg > 1:892466/1‑140 (MQ=255)
tctcGTTACCGGATAGTGCGATCCAGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAg > 2:1192059/1‑140 (MQ=255)
aCCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGcgcg > 1:1055871/1‑140 (MQ=255)
cGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAg > 2:1101937/1‑140 (MQ=255)
gATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTg > 1:690723/1‑140 (MQ=255)
aTAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTgg > 1:453612/1‑140 (MQ=255)
tAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGc < 2:213749/140‑1 (MQ=255)
tAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGc < 2:892119/140‑1 (MQ=255)
gTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCaa > 1:25417/1‑140 (MQ=255)
tGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAAc > 2:741334/1‑140 (MQ=255)
tGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAAc > 1:723584/1‑140 (MQ=255)
cGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACtt > 1:1327849/1‑140 (MQ=255)
cccGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCg < 2:1180019/140‑1 (MQ=255)
ccGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGa > 1:1581206/1‑140 (MQ=255)
cGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGaa < 2:105906/140‑1 (MQ=255)
cAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGc < 2:651234/140‑1 (MQ=255)
aGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGTCAGAAACg > 1:1096285/1‑140 (MQ=255)
ggggTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGtt < 2:1386108/140‑1 (MQ=255)
ggggTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGtt < 2:25417/140‑1 (MQ=255)
ggTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTtgt > 1:838619/1‑140 (MQ=255)
tAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTga > 1:820734/1‑140 (MQ=255)
aaCTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTgag > 1:1106299/1‑140 (MQ=255)
aCCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGAt > 1:606043/1‑140 (MQ=255)
cATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCa > 2:1412742/1‑140 (MQ=255)
cATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCa < 2:136942/140‑1 (MQ=255)
cATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCa > 2:1030385/1‑140 (MQ=255)
tGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAAc > 2:638829/1‑140 (MQ=255)
tGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAAc < 2:723584/140‑1 (MQ=255)
aaaaaTATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCg > 2:1059613/1‑140 (MQ=255)
ttGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAAt < 2:892466/140‑1 (MQ=255)
cGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGt > 1:1454950/1‑140 (MQ=255)
cGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGt > 2:1575994/1‑140 (MQ=255)
cGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGt > 1:257115/1‑140 (MQ=255)
cGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGt > 1:312192/1‑140 (MQ=255)
ggCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGtt > 2:62128/1‑140 (MQ=255)
gTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGTCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGTTGCGGGt < 2:1096285/140‑1 (MQ=255)
tGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGTTGCGGGTGGaaa < 2:654630/140‑1 (MQ=255)
|
GTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGTTGCGGGTGGAAA > NC_000913/1421880‑1422151
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
