Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,422,369	T→C	G129G (GGT→GGC)	ydaV →	Rac prophage; putative ATP‑binding protein YdaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,422,369	0	T	C	100.0%	128.0 / NA	36	G129G (GGT→GGC)	ydaV	Rac prophage; putative ATP‑binding protein YdaV
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/20); total (16/20)

ATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTTGATGAAA  >  NC_000913/1422234‑1422496

aTACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGa                                                                                                                             <  1:257610/140‑1 (MQ=255)

 tACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCCGCGGAAACCCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGAc                                                                                                                            <  2:750580/140‑1 (MQ=255)

 tACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGAc                                                                                                                            <  2:151469/140‑1 (MQ=255)

        tGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGGACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGAtt                                                                                                                     >  2:639779/1‑140 (MQ=255)

          aGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATtgt                                                                                                                   >  2:73709/1‑140 (MQ=255)

            tCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGGACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTgg                                                                                                                 <  1:639779/140‑1 (MQ=255)

              aGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAGAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGtt                                                                                                               >  1:74751/1‑140 (MQ=255)

               ggCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTa                                                                                                              >  2:78666/1‑140 (MQ=255)

               ggCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTa                                                                                                              >  1:422893/1‑140 (MQ=255)

                     aTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAGAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTgg                                                                                                        <  2:74751/140‑1 (MQ=255)

                     aTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTgg                                                                                                        <  1:73709/140‑1 (MQ=255)

                     aTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTgg                                                                                                        <  1:621142/140‑1 (MQ=255)

                          tCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGAt                                                                                                   <  2:422893/140‑1 (MQ=255)

                                       gatgaCCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTAt                                                                                              >  2:367604/1‑132 (MQ=255)

                                       gatgaCCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTAt                                                                                              <  1:367604/132‑1 (MQ=255)

                                             cGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACg                                                                                <  1:78666/140‑1 (MQ=255)

                                             cGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACg                                                                                <  2:609349/140‑1 (MQ=255)

                                               ggTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGcc                                                                              >  1:623773/1‑140 (MQ=255)

                                                   tACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGcc                                                                              <  1:22746/136‑1 (MQ=255)

                                                   tACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGcc                                                                              >  2:22746/1‑136 (MQ=255)

                                                         ttttGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGC‑‑CAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGAt                                                                  >  1:2938/1‑140 (MQ=255)

                                                                               ggTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAAtt                                              <  2:623773/140‑1 (MQ=255)

                                                                                      ggAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCgg                                       >  2:479202/1‑140 (MQ=255)

                                                                                         agaaCCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGaa                                    <  1:641794/140‑1 (MQ=255)

                                                                                          gaaCCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAAc                                   <  1:864475/140‑1 (MQ=255)

                                                                                                 acttagccAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGa                            <  2:2938/133‑1 (MQ=255)

                                                                                                     cGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTg                        <  2:657915/140‑1 (MQ=255)

                                                                                                        cAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGAt                     >  2:292779/1‑140 (MQ=255)

                                                                                                          gCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATct                   >  1:369394/1‑140 (MQ=255)

                                                                                                             aTCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTgg                       <  2:706379/133‑1 (MQ=255)

                                                                                                             aTCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTgg                       >  1:706379/1‑133 (MQ=255)

                                                                                                                        ccTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTtgatg     >  1:153700/1‑140 (MQ=255)

                                                                                                                         ctgctgAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTtgatga    <  2:153700/140‑1 (MQ=255)

                                                                                                                           gctgAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTTGATGaaa  >  1:784537/1‑140 (MQ=255)

                                                                                                                                 aGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTtgatga    >  2:546168/1‑132 (MQ=255)

                                                                                                                                 aGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTtgatga    <  1:546168/132‑1 (MQ=255)

ATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTTGATGAAA  >  NC_000913/1422234‑1422496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A