Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,338,438	A→G	V328A (GTT→GCT)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,338,438	0	A	G	100.0%	104.5 / NA	32	V328A (GTT→GCT)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/15); total (17/15)

TACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTT  >  NC_000913/2338307‑2338572

tACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAAc                                                                                                                                >  1:140140/1‑140 (MQ=255)

       ttCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCt                                                                                                                         >  2:860921/1‑140 (MQ=255)

             aaTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGt                                                                                                                   <  1:860921/140‑1 (MQ=255)

               tAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTag                                                                                                                 <  1:293804/140‑1 (MQ=255)

                 gTACGACGGGTCACCACTTCACGGCGGTGGCGAACAAACGCCGCGGTGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGGTACCGAAAGAAGCCTGCAACTGGGTCTGGGGGTagag                                                                                                               >  2:992095/1‑140 (MQ=255)

                  tACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAgg                                                                                                              >  1:212759/1‑140 (MQ=255)

                  gacgacgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAgg                                                                                                              <  1:406605/139‑1 (MQ=255)

                       cgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGgttgtt                                                                                                         <  2:212759/140‑1 (MQ=255)

                          gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAg                                                                                                      >  2:1026804/1‑140 (MQ=255)

                          gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAg                                                                                                      >  2:232879/1‑140 (MQ=255)

                          gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAg                                                                                                      <  1:87697/140‑1 (MQ=255)

                          gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAg                                                                                                      >  1:685211/1‑140 (MQ=255)

                               cacTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACaa                                                                                                 >  1:211043/1‑140 (MQ=255)

                                    cACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCa                                                                                            >  2:1006545/1‑140 (MQ=255)

                                            tGACGGACAAACCCCCCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCAAGGGGCAATGCCACCATATTGATACCGAAAGAAGCCTGCAACTGGGGCTGGGAGTAGAGGTTGTTGAGCACAACATTACCCACCGc                                                                                    >  2:755900/1‑140 (MQ=255)

                                             gACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCa                                                                                   <  2:685211/140‑1 (MQ=255)

                                                      aCGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTc                                                                          >  1:1046214/1‑140 (MQ=255)

                                                                  tgTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACg                                                              <  1:670767/140‑1 (MQ=255)

                                                                  tgTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACg                                                              <  2:366223/140‑1 (MQ=255)

                                                                           ggTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATa                                                     <  2:1046214/140‑1 (MQ=255)

                                                                             ttCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATAcc                                                   <  1:1026804/140‑1 (MQ=255)

                                                                                       tCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                                         >  2:437923/1‑140 (MQ=255)

                                                                                        cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                        <  1:437923/140‑1 (MQ=255)

                                                                                        cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                        >  1:298088/1‑140 (MQ=255)

                                                                                        cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                        >  2:326435/1‑140 (MQ=255)

                                                                                        cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                        <  2:397170/140‑1 (MQ=255)

                                                                                              aCCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTg                                            >  1:475458/1‑130 (MQ=255)

                                                                                              aCCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTg                                            <  2:475458/130‑1 (MQ=255)

                                                                                              aCCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGt                                  >  1:1030827/1‑140 (MQ=255)

                                                                                                  tGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACg                              <  2:1027408/140‑1 (MQ=255)

                                                                                                         aTGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGAc                                      <  2:1085604/125‑1 (MQ=255)

                                                                                                         aTGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGAc                                      >  1:1085604/1‑125 (MQ=255)

                                                                                                          tGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgcgc                      >  2:581389/1‑140 (MQ=255)

                                                                                                                         aCCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCAcgc       >  2:967503/1‑140 (MQ=255)

                                                                                                                            gaaagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtt    <  1:326435/140‑1 (MQ=255)

                                                                                                                             aaagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGttt   >  2:581187/1‑140 (MQ=255)

                                                                                                                              aagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGa                   >  2:46466/1‑123 (MQ=255)

                                                                                                                              aagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGa                   <  1:46466/123‑1 (MQ=255)

                                                                                                                              aagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtttt  >  2:597333/1‑140 (MQ=255)

                                                                                                                              aagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtttt  >  2:824205/1‑140 (MQ=255)

TACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTT  >  NC_000913/2338307‑2338572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A