Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,357,149	A→G	E82E (GAA→GAG)	rpnE →	inactive recombination‑promoting nuclease‑like protein RpnE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,357,149	0	A	G	100.0%	148.2 / NA	41	E82E (GAA→GAG)	rpnE	inactive recombination‑promoting nuclease‑like protein RpnE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/13); total (28/13)

CTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGAA  >  NC_000913/2357018‑2357281

cTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAg                                                                                                                              <  1:871561/140‑1 (MQ=255)

  ttGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGct                                                                                                                            <  1:454566/140‑1 (MQ=255)

     cAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTg                                                                                                                         <  1:563443/140‑1 (MQ=255)

          tGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGaa                                                                                                                    <  2:623888/140‑1 (MQ=255)

                           gAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGGGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAatat                                                                                                            >  2:659935/1‑131 (MQ=255)

                           gAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAatat                                                                                                            <  1:659935/131‑1 (MQ=255)

                           gAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCg                                                                                                   >  2:121666/1‑140 (MQ=255)

                                cACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTaa                                                                                              <  1:522211/140‑1 (MQ=255)

                                 aCTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAAt                                                                                             >  1:946137/1‑140 (MQ=255)

                                  cTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATg                                                                                            >  1:304740/1‑140 (MQ=255)

                                  cTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATg                                                                                            >  1:766808/1‑140 (MQ=255)

                                             gAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCAc                                                                                 <  2:766808/140‑1 (MQ=255)

                                                     tttACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCa                                                                         >  2:772328/1‑140 (MQ=255)

                                                             cTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGt                                                                 <  2:437907/140‑1 (MQ=255)

                                                                actactCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCAc                                                              <  2:411345/140‑1 (MQ=255)

                                                                 ctactCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCAcc                                                             <  2:78686/140‑1 (MQ=255)

                                                                          tGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAg                                                    >  1:253914/1‑140 (MQ=255)

                                                                                   gTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACa                                           >  2:294/1‑140 (MQ=255)

                                                                                        tGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACa                                                       >  2:162292/1‑123 (MQ=255)

                                                                                        tGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACa                                                       <  1:162292/123‑1 (MQ=255)

                                                                                         ggAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTcc                                     >  2:142177/1‑140 (MQ=255)

                                                                                           aaaCCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCg                                   <  2:253914/140‑1 (MQ=255)

                                                                                                aGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCtggt                              >  2:66671/1‑140 (MQ=255)

                                                                                                     cGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGc                         >  1:687145/1‑140 (MQ=255)

                                                                                                      ggTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGcc                        >  1:243542/1‑140 (MQ=255)

                                                                                                       gTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCg                       <  2:304740/140‑1 (MQ=255)

                                                                                                                       cTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATg       >  1:321/1‑140 (MQ=255)

                                                                                                                        tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  2:158482/1‑140 (MQ=255)

                                                                                                                        tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  1:365071/1‑140 (MQ=255)

                                                                                                                        tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  1:358664/1‑140 (MQ=255)

                                                                                                                        tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  2:747064/1‑140 (MQ=255)

                                                                                                                        tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  1:288123/1‑140 (MQ=255)

                                                                                                                         gCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGc     >  1:701397/1‑140 (MQ=255)

                                                                                                                          cGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCg    >  1:479712/1‑140 (MQ=255)

                                                                                                                          cGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTCCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCg    >  1:346126/1‑140 (MQ=255)

                                                                                                                           gTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGa   >  2:415235/1‑140 (MQ=255)

                                                                                                                            tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  1:884674/1‑140 (MQ=255)

                                                                                                                            tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  1:562630/1‑140 (MQ=255)

                                                                                                                            tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  1:505609/1‑140 (MQ=255)

                                                                                                                            tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  2:506607/1‑140 (MQ=255)

                                                                                                                            tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGACCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  2:420400/1‑140 (MQ=255)

CTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGAA  >  NC_000913/2357018‑2357281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A