Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,163,735	T→C	S747G (AGC→GGC)	parC ←	DNA topoisomerase IV subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,163,735	0	T	C	100.0%	113.6 / NA	33	S747G (AGC→GGC)	parC	DNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/15); total (18/15)

AACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCAA  >  NC_000913/3163598‑3163869

aacGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACcgccgc                                                                                                                                      >  2:183013/1‑140 (MQ=255)

    ttttCAAGGAATTTTTTATCGTTTCGGGGGGTTGAATACGCTGCCGGATGCCGACTAAACTTTTCACCCGGCGTTCCTTGCAGGGGGGAGGAAACGGCCCCCCCCCCGGCATATTCCTCTCCGCTATCACCGCCGCTGGc                                                                                                                                  <  2:876170/139‑1 (MQ=255)

     tttCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCa                                                                                                                                 >  2:498412/1‑140 (MQ=255)

        cAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACgg                                                                                                                              >  2:162218/1‑140 (MQ=255)

                   ttATCTTTCCGGGGGGTTGAATCCGCTGCGGGAGGACGACTTACCTTTTCACCCGGCTTTCTTTGCAGGGGGGAGAAAACGGCCCCCCCCCCGGCATATTCCTCTCCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTc                                                                                                                   <  2:123791/140‑1 (MQ=255)

                            ccGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTTGCACGGCGAGGAGAGTCGATCTCAac                                                                                                          <  1:183013/140‑1 (MQ=255)

                              gggggTTAAATCCGCGGCGGGATGACGATTTAACTTTTCACCGGGGTTTCCTGGAAGGGGGGAGAAACCGGCCCCCCCCCGGGAATTTTCCTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAacac                                                                                                        <  2:480750/140‑1 (MQ=255)

                                    tGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgatcga                                                                                                  >  1:480750/1‑140 (MQ=255)

                                    tGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgatcga                                                                                                  >  2:78907/1‑140 (MQ=255)

                                            cTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGc                                                                                          >  2:273248/1‑140 (MQ=255)

                                                      acgacTTATCTTTTCACCCGGCTTTCCTGGCAGGGGGGAGGAAACAGCCCCCCCCCCGGCATATTCCTCTCCGCTACCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCa                                                                                <  2:134308/140‑1 (MQ=255)

                                                                  ttCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACcgc                                                                    >  2:358426/1‑140 (MQ=255)

                                                                  ttCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACcgc                                                                    >  1:92176/1‑140 (MQ=255)

                                                                     aTCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCgcggc                                                                 >  2:91608/1‑140 (MQ=255)

                                                                           cGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCAc                                                           >  2:171044/1‑140 (MQ=255)

                                                                                 ttGCAGGGGGGAGAAAACGCCCCCCCCCCCGGCATTTTACTTTTCTCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGc                                                     <  2:92176/140‑1 (MQ=255)

                                                                                  tGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGcc                                                    >  1:631597/1‑140 (MQ=255)

                                                                                                     cgcCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAActct                                 >  1:725633/1‑140 (MQ=255)

                                                                                                     cgcCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAActct                                 >  2:179575/1‑140 (MQ=255)

                                                                                                          tCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCgg                            >  1:241358/1‑140 (MQ=255)

                                                                                                           ccccGGCATATTCCTCTCCCCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCggg                           <  2:941110/140‑1 (MQ=255)

                                                                                                            cccGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGgc                          <  2:631597/140‑1 (MQ=255)

                                                                                                             cgggCATATTCCTCTCCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGgcg                         <  2:914967/138‑1 (MQ=255)

                                                                                                                      tACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAAt                >  1:714942/1‑140 (MQ=255)

                                                                                                                      tACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAAt                >  1:25634/1‑140 (MQ=255)

                                                                                                                          ctTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTg            <  1:498412/140‑1 (MQ=255)

                                                                                                                           ttCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGc           >  1:150409/1‑140 (MQ=255)

                                                                                                                            ccGCTATCCCCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCg          <  2:223783/139‑1 (MQ=255)

                                                                                                                             cGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGt         >  1:34670/1‑140 (MQ=255)

                                                                                                                               cTATCCCCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGttt       <  1:740415/140‑1 (MQ=255)

                                                                                                                               cTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGttt       <  1:179575/140‑1 (MQ=255)

                                                                                                                                tATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTc      >  2:697626/1‑140 (MQ=255)

                                                                                                                                  tCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTccc    <  1:78907/140‑1 (MQ=255)

                                                                                                                                    cccgccgCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCaa  <  1:162218/139‑1 (MQ=255)

                                                                                                                                    aCCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCaa  <  2:34670/140‑1 (MQ=255)

                                                                                                                                    aCCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCaa  <  2:714942/140‑1 (MQ=255)

AACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCAA  >  NC_000913/3163598‑3163869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A