Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,938	T→C	T306A (ACT→GCT)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,938	0	T	C	100.0%	121.7 / NA	35	T306A (ACT→GCT)	chiA	endochitinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/4); total (31/4)

AAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCAT  >  NC_000913/3468809‑3469063

aattttCGCTCCCTTCCTGGCCGCTCCACGCTTGAAATGAGTAATCGTTAACGCTACCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTGGGGGTCCCGGGTGTTTCCGGAGTTGGATCACCCGGAGCCACAGCACCg                                                                                                                     <  2:110534/137‑1 (MQ=255)

        cTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCattatt                                                                                                             >  1:110534/1‑140 (MQ=255)

              tCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCgg                                                                                                       >  1:1001545/1‑140 (MQ=255)

                       cTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGa                                                                                              >  1:842791/1‑140 (MQ=255)

                        tCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGaa                                                                                             >  2:543306/1‑140 (MQ=255)

                        tCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGaa                                                                                             >  1:450280/1‑140 (MQ=255)

                         ccACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAAc                                                                                            <  2:619443/140‑1 (MQ=255)

                             gCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGa                                                                                        >  2:457742/1‑140 (MQ=255)

                              cTTGCAATGAGTAATCGTTAACGCTTCCCGGTGGTGTTGATGGCTCGCTGTTACCCGGAGTTGGGGTCCCCGGTTTTTCGGGAGTTGGACCACCGGGAGCCCCAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAg                                                                                       <  2:842791/140‑1 (MQ=255)

                              cTTGCAATGAGAATTCGTTAGCGTTACCCGCGGGTGTTAGGGGCTCGTTGTTACCCGGGGTGGGGTGCACCGTTGTTCCGGGATTTGGATCACCGGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAg                                                                                       <  2:450280/140‑1 (MQ=255)

                                tGCAATGAGTAATCGTAACCGCTATCCGCTGGTGTTGATGGCTCGCTTTTACCGGGGGTGGGGGTCCCGGGTTTTTCGGGAGTTGGACCAACCGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGcc                                                                                     <  2:865987/140‑1 (MQ=255)

                                 gaaaTGAGTAATCGTTAACGCTACCCGCTGGTTTTGAGGGCTCGCTTTTATCCGGAGTGGGGGTCACGGGTTTTCCGGGAGTTGGACCACCGGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCa                                                                                    <  2:354163/138‑1 (MQ=255)

                                  aaaTGAGTAATCGTTAACGCTACCCGTTGGTGTTGATGGCTCGCTTTTATCGGGAGTGGGGGTCCCCGGTTTTCCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAg                                                                                   <  2:429473/139‑1 (MQ=255)

                                       aGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTa                                                                              >  1:865987/1‑140 (MQ=255)

                                         tAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAcc                                                                            >  2:1024057/1‑140 (MQ=255)

                                                 aaCGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGc                                                                    >  1:834726/1‑140 (MQ=255)

                                                   cGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTg                                                                  >  1:722190/1‑140 (MQ=255)

                                                   cGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTg                                                                  >  2:156317/1‑140 (MQ=255)

                                                       aTCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGAttt                                                              >  1:298827/1‑140 (MQ=255)

                                                        tCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTc                                                             >  1:723777/1‑140 (MQ=255)

                                                        tCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTc                                                             >  1:129925/1‑140 (MQ=255)

                                                             tGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttg                                                        >  1:954461/1‑140 (MQ=255)

                                                             tGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttg                                                        >  2:623088/1‑140 (MQ=255)

                                                                tgtTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccg                                                     >  1:112491/1‑140 (MQ=255)

                                                                  ttGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccgtt                                                   >  1:568531/1‑140 (MQ=255)

                                                                     aTGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccgttgcc                                                >  2:458002/1‑140 (MQ=255)

                                                                        gCTCGCTTTTATCGGGAGTTGGGGTCCCGGGTGTTTCGGGAGTTGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcg                                             <  2:423134/140‑1 (MQ=255)

                                                                         cTCGTTTTTATCGGGAGTGGGGGTCCCCGGTGTTTCGGGAGTTGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            <  2:723777/140‑1 (MQ=255)

                                                                         cTCGCTGTTATCGGGAGTGGGGGTCCCGGGTTTTCCCGGAGTTGGATCACCGGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            <  2:568531/140‑1 (MQ=255)

                                                                         cTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            >  2:118621/1‑140 (MQ=255)

                                                                         cTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            >  1:441375/1‑140 (MQ=255)

                                                                         cTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            >  1:423134/1‑140 (MQ=255)

                                                                               tttATCGGGAGTGGGGGCCCCGGGTTTTTCCGGGGTGGGATCACCGGGCGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAt                                      <  2:638299/139‑1 (MQ=255)

                                                                               gTTATCGGGAGTGGGGGCCCCCGGTGTTCCCGGAGTTGGACCACCGGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAt                                      <  2:722190/140‑1 (MQ=255)

                                                                                   ccgggAGTGGGGGTCACGGGTGTTCCCGGAGTTGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACg                                  <  2:298827/137‑1 (MQ=255)

                                                                                     cGGGGTTGGGGCCCCCGGTTTTTCCGGTTTTGGATCACCCGGGGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGcc                                <  2:112491/140‑1 (MQ=255)

                                                                                      ggAGTGGGGGTCCCGGGTTTTCCGGGAGTGGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCa                               <  2:1001545/140‑1 (MQ=255)

                                                                                        aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAt                                      <  2:850469/131‑1 (MQ=255)

                                                                                        aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAt                                      >  1:850469/1‑131 (MQ=255)

                                                                                        aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATg                             >  1:871239/1‑140 (MQ=255)

                                                                                        aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATg                             >  1:139562/1‑140 (MQ=255)

                                                                                          ttGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATggg                           >  1:1052218/1‑140 (MQ=255)

                                                                                          ttGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATggg                           >  1:797858/1‑140 (MQ=255)

                                                                                          ttGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATggg                           >  1:252929/1‑140 (MQ=255)

                                                                                             gggTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCG‑ACATAACGCCATGGGTTAc                       >  1:847130/1‑140 (MQ=255)

                                                                                                   ccGGTTTTTCCGGAGTTGGATCACCGGGAGCCACCGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCa                  <  1:942554/140‑1 (MQ=39)

                                                                                                          ttCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtttt           >  2:583477/1‑140 (MQ=255)

                                                                                                                 gTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGc    <  2:834726/140‑1 (MQ=255)

                                                                                                                 gTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGc    <  1:156317/140‑1 (MQ=255)

                                                                                                                   tGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGAATCGTTTTCTTTCGCAt  >  2:1003455/1‑140 (MQ=255)

AAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCAT  >  NC_000913/3468809‑3469063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A