Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 134 60.5 2175152 94.0% 2044642 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,885,773 A→G Y233C (TAC→TGC)  yidC → membrane protein insertase YidC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,885,7730AG100.0% 184.8 / NA 51Y233C (TAC→TGC) yidCmembrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

GCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCG  >  NC_000913/3885636‑3885904
                                                                                                                                         |                                                                                                                                   
gCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCACGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCa                                                                                                                                   >  2:993255/1‑140 (MQ=255)
  tGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAg                                                                                                                                 >  2:783621/1‑140 (MQ=255)
            ctcGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGtt                                                                                                                               <  2:22694/132‑1 (MQ=255)
            ctcGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGtt                                                                                                                               >  1:22694/1‑132 (MQ=255)
                  tGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCg                                                                                                                 >  2:535297/1‑140 (MQ=255)
                   ggTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGa                                                                                                                <  1:931548/140‑1 (MQ=255)
                        gTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACg                                                                                                           <  2:334605/140‑1 (MQ=255)
                        gTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACg                                                                                                           <  1:535297/140‑1 (MQ=255)
                         ttGAAGCAATCCATCACTCTGCCACCGCATCTCGATACTGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGa                                                                                                          >  2:688315/1‑140 (MQ=255)
                          tGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGaa                                                                                                         <  1:207004/140‑1 (MQ=255)
                          tGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGaa                                                                                                         <  1:377014/140‑1 (MQ=255)
                                 aTCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGa                                                                                                  >  2:455684/1‑140 (MQ=255)
                                   ccATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAAc                                                                                                <  2:1087455/140‑1 (MQ=255)
                                      tCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtc                                                                                             <  1:224181/140‑1 (MQ=255)
                                      tCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTACACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtc                                                                                             <  1:239724/140‑1 (MQ=255)
                                       cACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtct                                                                                            >  1:571659/1‑140 (MQ=255)
                                            tGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTg                                                                                                   >  1:38475/1‑128 (MQ=255)
                                            tGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTg                                                                                                   <  2:38475/128‑1 (MQ=255)
                                            tGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCg                                                                                       <  1:783621/140‑1 (MQ=255)
                                              ccACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaa                                                                                     <  2:571659/140‑1 (MQ=255)
                                              ccACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaa                                                                                     >  1:219235/1‑140 (MQ=255)
                                              ccACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaa                                                                                     <  1:2789/140‑1 (MQ=255)
                                                    cATCTCGATACTGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAggtgg                                                                               <  1:688315/140‑1 (MQ=255)
                                                    cATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAggtgg                                                                               >  1:912705/1‑140 (MQ=255)
                                                         cGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCACGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTggg                                                                          <  1:993255/140‑1 (MQ=255)
                                                             aCCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGc                                                                      <  2:98577/140‑1 (MQ=255)
                                                             aCCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGc                                                                      <  2:509398/140‑1 (MQ=255)
                                                               cGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGa                                                                    >  1:874693/1‑140 (MQ=255)
                                                                  aaGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGAtgc                                                                 <  2:105333/140‑1 (MQ=255)
                                                                    gcagcaACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGAtgctg                                                               <  2:352184/140‑1 (MQ=255)
                                                                     cagcaACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGAtgctgc                                                              <  2:219235/140‑1 (MQ=255)
                                                                        caACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAAc                                                           >  1:65317/1‑140 (MQ=255)
                                                                              cGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTAtt                                                     >  1:807364/1‑140 (MQ=255)
                                                                                cACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTc                                                   >  1:514994/1‑140 (MQ=255)
                                                                                   tGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTcgcg                                                <  2:874693/140‑1 (MQ=255)
                                                                                    gCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGa                                               <  2:807364/140‑1 (MQ=255)
                                                                                     cacaCCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGAc                                              >  2:702967/1‑140 (MQ=255)
                                                                                          cTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGt                                         <  2:583066/140‑1 (MQ=255)
                                                                                              cGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGAt                                     <  1:455684/140‑1 (MQ=255)
                                                                                                    gcggcgTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCa                               >  1:968083/1‑140 (MQ=255)
                                                                                                    gcggcgTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCa                               >  1:968989/1‑140 (MQ=255)
                                                                                                      ggcgTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATa                             >  1:71481/1‑140 (MQ=255)
                                                                                                        cgTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAac                           >  2:280394/1‑140 (MQ=255)
                                                                                                          tACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAacga                         <  2:514994/140‑1 (MQ=255)
                                                                                                             tCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACgg                      <  2:968083/140‑1 (MQ=255)
                                                                                                                 cGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTAcc                  >  1:635023/1‑140 (MQ=255)
                                                                                                                    cTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACcaac               >  2:545412/1‑140 (MQ=255)
                                                                                                                            aaGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCta       <  2:968989/140‑1 (MQ=255)
                                                                                                                            aaGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCta       >  2:187183/1‑140 (MQ=255)
                                                                                                                             aGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCtat      >  1:870359/1‑140 (MQ=255)
                                                                                                                                 tGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCg  >  1:315372/1‑140 (MQ=255)
                                                                                                                                 tGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCg  <  2:320243/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                   
GCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCG  >  NC_000913/3885636‑3885904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A