Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,886,078	T→C	F335L (TTC→CTC)	yidC →	membrane protein insertase YidC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,886,078	0	T	C	95.0%	268.3 / ‑1.0	80	F335L (TTC→CTC)	yidC	membrane protein insertase YidC
Reads supporting (aligned to +/- strand): ref base T (1/3); new base C (36/40); total (37/43)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACAC  >  NC_000913/3885946‑3886208

cAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCAtctc                                                                                                                             >  2:795651/1‑140 (MQ=255)

 aGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCAtctct                                                                                                                            >  2:830904/1‑140 (MQ=255)

   ccGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCa                                                                                                                          >  2:372672/1‑140 (MQ=255)

   ccGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCa                                                                                                                          >  1:748199/1‑140 (MQ=255)

            gTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGtt                                                                                                                 >  2:804496/1‑140 (MQ=255)

              tCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCa                                                                                                               <  1:812876/140‑1 (MQ=255)

              tCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCa                                                                                                               <  1:795651/140‑1 (MQ=255)

              tCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCa                                                                                                               >  2:812876/1‑140 (MQ=255)

                tgCCTGGTCAGACTGGAGCGATGAAAAGCCCCCTGTGGGTTGGCCCGGAAATCCAGGAAAAAATGGCAGCTGTTGCTCCGCCCCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTCaaa                                                                                                             <  2:496143/139‑1 (MQ=255)

                 gCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCg                                                                                                                      <  1:146971/130‑1 (MQ=255)

                 gCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCg                                                                                                                      >  2:146971/1‑130 (MQ=255)

                 gCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAAc                                                                                                            >  2:461085/1‑140 (MQ=255)

                 gCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAAc                                                                                                            >  1:923574/1‑140 (MQ=255)

                 gCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAAc                                                                                                            >  1:652957/1‑140 (MQ=255)

                   cTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAActg                                                                                                          <  1:804496/140‑1 (MQ=255)

                      gTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGcc                                                                                                                       <  1:160028/124‑1 (MQ=255)

                      gTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGcc                                                                                                                       >  2:160028/1‑124 (MQ=255)

                      gTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAActgctg                                                                                                       >  2:585058/1‑140 (MQ=255)

                      gTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAActgctg                                                                                                       >  1:126905/1‑140 (MQ=255)

                      gTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAActgctg                                                                                                       >  2:132257/1‑140 (MQ=255)

                       tCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGa                                                                                                      >  1:851996/1‑140 (MQ=255)

                          gACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAAt                                                                                                   <  1:479189/140‑1 (MQ=255)

                          gACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAAt                                                                                                   <  1:826724/140‑1 (MQ=255)

                            cTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATgg                                                                                                 <  2:748199/140‑1 (MQ=255)

                                cgcgATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATcc                                                                                             >  2:859680/1‑140 (MQ=255)

                                cgcgATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATcc                                                                                             >  2:658518/1‑140 (MQ=255)

                                cgcgATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATcc                                                                                             <  1:543485/140‑1 (MQ=255)

                                   gATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATa                                                                                          <  2:923574/140‑1 (MQ=255)

                                     tGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGc                                                                                        >  2:206120/1‑140 (MQ=255)

                                      gAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCt                                                                                       <  1:202227/140‑1 (MQ=255)

                                      gAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCt                                                                                       <  1:658518/140‑1 (MQ=255)

                                        aCAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCttt                                                                                     <  1:830904/140‑1 (MQ=255)

                                             aCCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTggg                                                                                <  2:633607/140‑1 (MQ=255)

                                             aCCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTggg                                                                                <  1:206120/140‑1 (MQ=255)

                                                 tgtgGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAAc                                                                            >  2:54101/1‑140 (MQ=255)

                                                 tgtgGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAAc                                                                            >  2:811606/1‑140 (MQ=255)

                                                 tgtgGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAAc                                                                            >  2:706455/1‑140 (MQ=255)

                                                  gtgGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACt                                                                           <  1:372672/140‑1 (MQ=255)

                                                      gTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTgggg                                                                       <  2:878713/140‑1 (MQ=255)

                                                         ggCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCtt                                                                    <  2:851996/140‑1 (MQ=255)

                                                         ggCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCtt                                                                    >  1:345670/1‑140 (MQ=255)

                                                         ggCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCtt                                                                    >  1:335644/1‑140 (MQ=255)

                                                         ggCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTAGGGCtt                                                                    >  2:10622/1‑140 (MQ=255)

                                                          gCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTtc                                                                   >  1:744893/1‑140 (MQ=255)

                                                           cccGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTtct                                                                  >  2:551060/1‑140 (MQ=255)

                                                           cccGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTtct                                                                  >  1:313558/1‑140 (MQ=255)

                                                           cccGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTtct                                                                  <  1:706455/140‑1 (MQ=255)

                                                             cGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTcc                                                                >  2:905919/1‑140 (MQ=255)

                                                              ggAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCa                                                               <  1:811606/140‑1 (MQ=255)

                                                               gAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCAt                                                              >  1:834011/1‑140 (MQ=255)

                                                               gAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCAt                                                              >  2:462850/1‑140 (MQ=255)

                                                                 aaTCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTa                                                            <  2:253474/140‑1 (MQ=255)

                                                                          cAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTAtcatcatca                                                   <  1:132257/140‑1 (MQ=255)

                                                                             aaTGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCt                                                >  2:810601/1‑140 (MQ=255)

                                                                                ggCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTATCCATTATCATCATCACCTTTa                                             <  1:583177/140‑1 (MQ=255)

                                                                                 gCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTAt                                            <  1:905919/140‑1 (MQ=255)

                                                                                 gCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTAt                                            <  2:345670/140‑1 (MQ=255)

                                                                                  cAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTAtc                                           <  1:585058/140‑1 (MQ=255)

                                                                                     cTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTAtcgtt                                        <  2:126905/140‑1 (MQ=255)

                                                                                         tGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTg                                    <  1:859680/140‑1 (MQ=255)

                                                                                         tGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTg                                    <  2:335644/140‑1 (MQ=255)

                                                                                         tGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTg                                    <  1:10622/140‑1 (MQ=255)

                                                                                          gCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGTTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTgg                                   <  2:652957/140‑1 (MQ=255)

                                                                                          gCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTgg                                   <  2:313558/140‑1 (MQ=255)

                                                                                           cTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGc                                  <  1:461085/140‑1 (MQ=255)

                                                                                                cACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGcatcat                             >  1:129211/1‑140 (MQ=255)

                                                                                                  ccTGGATCTGACCGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGt                           >  2:590053/1‑140 (MQ=255)

                                                                                                             ccGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGcatca                              >  1:191570/1‑126 (MQ=255)

                                                                                                             ccGTTGATTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGcatca                              <  2:191570/126‑1 (MQ=255)

                                                                                                                   aTTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAgcg          <  2:502077/140‑1 (MQ=255)

                                                                                                                   aTTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAgcg          <  1:462850/140‑1 (MQ=255)

                                                                                                                   aTTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAgcg          <  2:744893/140‑1 (MQ=255)

                                                                                                                   aTTACGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAgcg          <  2:269701/140‑1 (MQ=255)

                                                                                                                      aCGGTTGGTTGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAg       >  2:409338/1‑140 (MQ=255)

                                                                                                                        ggttggttGTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTa     <  2:834011/140‑1 (MQ=255)

                                                                                                                           tggttgTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTacac  <  2:341565/140‑1 (MQ=255)

                                                                                                                             gttgTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATGGTTCGTGGCATCATGTACCCGCTGACCa               >  2:219966/1‑125 (MQ=255)

                                                                                                                             gttgTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATGGTTCGTGGCATCATGTACCCGCTGACCa               <  1:219966/125‑1 (MQ=255)

                                                                                                                             gttgTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCCTTATCGTTCGTGGCATCATGTACCCGCTGAc                 >  2:560389/1‑123 (MQ=255)

                                                                                                                             gttgTGGCTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCCTTATCGTTCGTGGCATCATGTACCCGCTGAc                 <  1:560389/123‑1 (MQ=255)

CAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTCAAACTGCTGAAATGGATCCATAGCTTTGTGGGTAACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACAC  >  NC_000913/3885946‑3886208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A