Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	665,137	A→G	C18C (TGT→TGC)	rlpA ←	rare lipoprotein RlpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	665,137	0	A	G	100.0%	167.8 / NA	48	C18C (TGT→TGC)	rlpA	rare lipoprotein RlpA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/22); total (26/22)

GCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGC  >  NC_000913/665004‑665270

gcTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGcc                                                                                                                                 <  2:265537/140‑1 (MQ=255)

gcTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGcc                                                                                                                                 <  2:421409/140‑1 (MQ=255)

              tttGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGc                                                                                                                   >  1:85493/1‑140 (MQ=255)

                 gCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCcgc                                                                                                                <  1:85840/140‑1 (MQ=255)

                                 gTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGc                                                                                                >  2:279704/1‑140 (MQ=255)

                                  ttCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGcc                                                                                               <  1:898040/140‑1 (MQ=255)

                                  ttCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGcc                                                                                               <  2:19605/140‑1 (MQ=255)

                                   tCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCa                                                                                              <  2:85493/140‑1 (MQ=255)

                                    cGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCAc                                                                                             <  1:260031/140‑1 (MQ=255)

                                    cGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCAc                                                                                             <  2:647788/140‑1 (MQ=255)

                                           cgGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTAc                                                                                      >  1:400996/1‑140 (MQ=255)

                                                      cGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCGTGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCAc                                                                           <  2:543277/140‑1 (MQ=255)

                                                                aaCTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGGGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACg                                                                 >  1:244625/1‑140 (MQ=255)

                                                                        ggCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGa                                                         >  1:156944/1‑140 (MQ=255)

                                                                            gTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGacaac                                                     >  1:887221/1‑140 (MQ=255)

                                                                             ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGa                                                         <  1:71341/135‑1 (MQ=255)

                                                                             ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGa                                                         >  2:71341/1‑135 (MQ=255)

                                                                                  tACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTc                                               <  2:887221/140‑1 (MQ=255)

                                                                                    ccGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCt                                             <  1:428427/140‑1 (MQ=255)

                                                                                     cGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACAGGCTTTTCGACAACATTTTCCtg                                            >  1:671846/1‑140 (MQ=255)

                                                                                     cGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtg                                            <  2:319517/140‑1 (MQ=255)

                                                                                     cGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtg                                            >  1:34116/1‑140 (MQ=255)

                                                                                      gCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgt                                           >  1:581446/1‑140 (MQ=255)

                                                                                       cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAGCATTTTCCtgtg                                          >  1:492622/1‑140 (MQ=255)

                                                                                       cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgtg                                          >  2:429862/1‑140 (MQ=255)

                                                                                       cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgtg                                          >  1:195833/1‑140 (MQ=255)

                                                                                       cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgtg                                          >  1:48006/1‑140 (MQ=255)

                                                                                       cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgt                                           >  1:756245/1‑139 (MQ=255)

                                                                                       cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgt                                           <  2:756245/139‑1 (MQ=255)

                                                                                       cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCt                                             >  1:786088/1‑137 (MQ=255)

                                                                                       cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCt                                             <  2:786088/137‑1 (MQ=255)

                                                                                         ggCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTggg                                        <  2:400996/140‑1 (MQ=255)

                                                                                          gCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGgt                                       >  1:664470/1‑140 (MQ=255)

                                                                                           cTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGgtg                                      >  1:791550/1‑140 (MQ=255)

                                                                                           cTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGgtg                                      >  1:713197/1‑140 (MQ=255)

                                                                                                 tACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGAtt                                <  2:156944/140‑1 (MQ=255)

                                                                                                      ttACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACAt                           >  2:908570/1‑140 (MQ=255)

                                                                                                        aCCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTa                         >  1:389276/1‑140 (MQ=255)

                                                                                                              tGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGATCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATcc                   >  2:602231/1‑140 (MQ=255)

                                                                                                              tGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATcc                   >  2:786336/1‑140 (MQ=255)

                                                                                                                ttGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCg                 <  2:581446/140‑1 (MQ=255)

                                                                                                                    tGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAAcc             >  2:873332/1‑140 (MQ=255)

                                                                                                                      aCCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa           <  1:602231/140‑1 (MQ=255)

                                                                                                                      aCCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa           >  1:320909/1‑140 (MQ=255)

                                                                                                                      aCCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa           <  1:279704/140‑1 (MQ=255)

                                                                                                                          tcatcGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTa                         >  1:334292/1‑122 (MQ=255)

                                                                                                                          tcatcGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTa                         <  2:334292/122‑1 (MQ=255)

                                                                                                                          tcatcGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCa       <  1:429862/140‑1 (MQ=255)

                                                                                                                               gCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGc  <  2:195833/140‑1 (MQ=255)

GCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGC  >  NC_000913/665004‑665270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A