Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 162 93.0 3098984 99.0% 3067994 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 670,318 A→G T85T (ACT→ACC)  nadD ← nicotinate‑mononucleotide adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913670,3180AG97.7% 305.3 / ‑3.1 87T85T (ACT→ACC) nadDnicotinate‑mononucleotide adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (47/37);  total (49/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTA  >  NC_000913/670182‑670453
                                                                                                                                        |                                                                                                                                       
cGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGgtg                                                                                                                                      <  1:1538711/140‑1 (MQ=255)
         gtgCATTGTCGAGTATCGTTTCGTATTCGTACCAGGACGGAAAGGACAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAgggg                                                                                                                             >  1:730383/1‑140 (MQ=255)
            cATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCa                                                                                                                          <  1:775816/140‑1 (MQ=255)
             aTTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAggggcatt                                                                                                                         >  2:620775/1‑136 (MQ=255)
             aTTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCAt                                                                                                                         >  2:289460/1‑140 (MQ=255)
              ttGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCAtt                                                                                                                        <  2:226136/140‑1 (MQ=255)
                 tCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTgcg                                                                                                                     >  2:843873/1‑140 (MQ=255)
                    aGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCtt                                                                                                                  >  2:1507123/1‑140 (MQ=255)
                    aGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCtt                                                                                                                  >  1:207404/1‑140 (MQ=255)
                     gTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCttt                                                                                                                 <  1:107587/140‑1 (MQ=255)
                     gTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCttt                                                                                                                 <  1:109850/140‑1 (MQ=255)
                      tATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTa                                                                                                                >  1:1458286/1‑140 (MQ=255)
                           tttCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTcg                                                                                                           <  1:216919/140‑1 (MQ=255)
                           tttCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTcg                                                                                                           >  2:50882/1‑140 (MQ=255)
                           tttCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTcg                                                                                                           >  2:982591/1‑140 (MQ=255)
                             tcgtattcgtcCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTcgcg                                                                                                         <  2:719469/140‑1 (MQ=255)
                             tcgtattcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTcgcg                                                                                                         <  1:1422091/140‑1 (MQ=255)
                                tattcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtc                                                                                                      <  1:1089851/140‑1 (MQ=255)
                                tattcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtc                                                                                                      >  1:1134392/1‑140 (MQ=255)
                                   tcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGTCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtcatc                                                                                                   <  2:954514/140‑1 (MQ=255)
                                   tcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtcatc                                                                                                   >  2:1408110/1‑140 (MQ=255)
                                   tcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtcatc                                                                                                   <  1:372963/140‑1 (MQ=255)
                                   tcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtcatc                                                                                                   >  1:883645/1‑140 (MQ=255)
                                    cgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtcatca                                                                                                  <  1:1279873/140‑1 (MQ=255)
                                     gtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCaa                                                                                                 <  1:1461631/140‑1 (MQ=255)
                                      taCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAag                                                                                                >  1:940608/1‑140 (MQ=255)
                                      taCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAag                                                                                                >  2:891658/1‑140 (MQ=255)
                                        ccAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAagag                                                                                              >  1:823847/1‑140 (MQ=255)
                                           ggTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTaa                                                                                           >  2:1399728/1‑140 (MQ=255)
                                           ggTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTaa                                                                                           >  2:1062603/1‑140 (MQ=255)
                                             tCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAaat                                                                                         >  1:1182776/1‑140 (MQ=255)
                                             tCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAaat                                                                                         >  1:1317235/1‑140 (MQ=255)
                                             tCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAaat                                                                                         >  2:536191/1‑140 (MQ=255)
                                                gAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAaataat                                                                                      >  2:1023365/1‑140 (MQ=255)
                                                gAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAaataat                                                                                      >  1:1134287/1‑140 (MQ=255)
                                                     gTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCtt                                                                                 >  1:1438555/1‑140 (MQ=255)
                                                       cagcagTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGt                                                                               <  1:289460/140‑1 (MQ=255)
                                                       cagcagTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGt                                                                               >  2:222117/1‑140 (MQ=255)
                                                           agTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGc                                                                           <  1:1197036/140‑1 (MQ=255)
                                                           agTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGc                                                                           <  1:1463547/140‑1 (MQ=255)
                                                                aTCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGA‑GGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGc                                                                     <  1:620775/140‑1 (MQ=255)
                                                                 tCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGc                                                                     <  1:1391308/140‑1 (MQ=255)
                                                                 cccTGCCCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGc                                                                     <  2:1091648/139‑1 (MQ=255)
                                                                    tGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGcccg                                                                  >  2:1407519/1‑138 (MQ=255)
                                                                    tGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCCGCCATCGCCAg                                                                  >  2:141287/1‑140 (MQ=255)
                                                                       ccAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTTTCGGCAATCGCCAGTTc                                                               >  1:230186/1‑140 (MQ=255)
                                                                       ccAAAAATAATCACGAGCGGCTCGTCGGTTTTTTGTCCCTTCCGCCTCGCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGTATTGCGCTTTAGCTCGCGTTGATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTc                                                               <  2:374750/140‑1 (MQ=255)
                                                                         aataatAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCaa                                                             <  2:207404/140‑1 (MQ=255)
                                                                           taataaACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGc                                                           >  2:1099877/1‑140 (MQ=255)
                                                                           taataaACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGc                                                           >  2:944506/1‑140 (MQ=255)
                                                                            aataaGCGCCAGCGGCCGGCCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCa                                                          <  2:677291/140‑1 (MQ=255)
                                                                            aataaACGCCAGCGGCAGGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCa                                                          <  1:1399728/140‑1 (MQ=255)
                                                                            aataaACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCa                                                          <  2:595986/140‑1 (MQ=255)
                                                                                aaCGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCAtgtg                                                      >  2:137715/1‑140 (MQ=255)
                                                                                  cGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGtt                                                    >  1:1005270/1‑140 (MQ=255)
                                                                                     cAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTAc                                                 >  2:82724/1‑140 (MQ=255)
                                                                                     cAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTAc                                                 >  1:149401/1‑140 (MQ=255)
                                                                                     cAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTAc                                                 >  2:1397901/1‑140 (MQ=255)
                                                                                     cAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTAc                                                 >  1:627256/1‑140 (MQ=255)
                                                                                     cAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTAc                                                 >  2:1088948/1‑140 (MQ=255)
                                                                                     cAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTAc                                                 >  1:1413102/1‑140 (MQ=255)
                                                                                     cAGCGGCACGACCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCCGCAATTGCCAGGTCAAGCATGTGTTTAc                                                 >  2:497712/1‑140 (MQ=255)
                                                                                      aGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACg                                                >  2:1367795/1‑140 (MQ=255)
                                                                                        cGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCt                                              >  1:1220147/1‑140 (MQ=255)
                                                                                        cGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCt                                              >  2:1021336/1‑140 (MQ=255)
                                                                                           cACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCa                                           <  2:974716/140‑1 (MQ=255)
                                                                                                  ggTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGtt                                    <  2:1317235/140‑1 (MQ=255)
                                                                                                  ggTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGtt                                    <  2:1182776/140‑1 (MQ=255)
                                                                                                   gTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATAAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTc                                   >  2:1396331/1‑140 (MQ=255)
                                                                                                       ttGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCtt                               <  1:536191/140‑1 (MQ=255)
                                                                                                        ttttCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTc                              <  1:1054317/138‑1 (MQ=255)
                                                                                                        tGTCCCTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTc                              <  1:1400484/140‑1 (MQ=255)
                                                                                                             cTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCt                         <  1:714696/140‑1 (MQ=255)
                                                                                                             cTGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCt                         <  1:982591/140‑1 (MQ=255)
                                                                                                              tGCCGCCACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTg                        >  1:660622/1‑140 (MQ=255)
                                                                                                                ccgccACTCTTTCAGTGTTTGCGCTGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTggg                      >  2:1148686/1‑140 (MQ=255)
                                                                                                                ccgccACTCTTTCAGTGTTTGAGCGGTGTAAGA‑GGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGACAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTgggg                     >  1:1447916/1‑140 (MQ=255)
                                                                                                                  gccACTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGa                    <  1:1062603/140‑1 (MQ=255)
                                                                                                                     aCTCTTTCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCCGGTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGa                 >  2:768504/1‑140 (MQ=255)
                                                                                                                         tttCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCg             >  2:1191053/1‑140 (MQ=255)
                                                                                                                          ttCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCCGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCgg            >  2:764002/1‑140 (MQ=255)
                                                                                                                           tCAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCgga           <  1:141287/140‑1 (MQ=255)
                                                                                                                            cAGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCggag          <  1:843873/140‑1 (MQ=255)
                                                                                                                             aGTGTTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCggagg         <  1:82724/140‑1 (MQ=255)
                                                                                                                               tgtTTTCGCGGTCTATGAGGGGTCTTTGCGTTTTAGCTCGCGTTTATCAAGAGTAAATAATGGCTTGTCGGTAATCGCCAGTTCAAGCATGTTTTTTCGCTGCTCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGaa       <  2:730383/140‑1 (MQ=255)
                                                                                                                               tgtTTGCGGGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGaa       <  2:823847/140‑1 (MQ=255)
                                                                                                                                gtTTGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAAc      <  2:1458286/140‑1 (MQ=255)
                                                                                                                                 tttGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACa     >  1:1491658/1‑140 (MQ=255)
                                                                                                                                  ttGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCTTGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACat    <  2:1134287/140‑1 (MQ=255)
                                                                                                                                   tGCGCGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACatt   >  1:452001/1‑140 (MQ=255)
                                                                                                                                    gcgcGGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACatta  <  1:1517037/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTA  >  NC_000913/670182‑670453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A