Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,359,053	A→G	G45G (GGT→GGC)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,359,053	0	A	G	100.0%	132.8 / NA	38	G45G (GGT→GGC)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/18); total (20/18)

CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATATTCAGTGGTGAATTAATAGCC  >  NC_000913/1358918‑1359185

cgcgTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCaa                                                                                                                                  >  2:273650/1‑140 (MQ=255)

cgcgTAGCTATAGGCCGAACCGGCCCCCGGAAACAGGCGAACCAGTTTGCTGTAGCTGATGGCGGTAAACAGCGCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCaa                                                                                                                                  <  2:612236/140‑1 (MQ=255)

  cgTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCaaaa                                                                                                                                <  2:140101/140‑1 (MQ=255)

     aGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTa                                                                                                                             <  1:494473/140‑1 (MQ=255)

      ggTATAGGCCGACCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGTCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTAt                                                                                                                            <  2:656492/140‑1 (MQ=255)

                 aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  2:909000/1‑140 (MQ=255)

                 aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  2:208019/1‑140 (MQ=255)

                 aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  1:334500/1‑140 (MQ=255)

                 aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  2:30106/1‑140 (MQ=255)

                 aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAAAGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  1:922583/1‑140 (MQ=255)

                 aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCCCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  2:841975/1‑140 (MQ=255)

                   ccGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCAt                                                                                                               <  1:30106/140‑1 (MQ=255)

                      gCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCgg                                                                                                            >  1:513624/1‑140 (MQ=255)

                      gCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCgg                                                                                                            >  1:383261/1‑140 (MQ=255)

                        cTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCg                                                                                                          <  1:289199/140‑1 (MQ=255)

                        cTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCg                                                                                                          <  1:208019/140‑1 (MQ=255)

                             gAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTgaga                                                                                                     <  1:909000/140‑1 (MQ=255)

                                 cTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAgg                                                                                                 <  2:513624/140‑1 (MQ=255)

                                 cTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAgg                                                                                                 <  2:383261/140‑1 (MQ=255)

                                    gCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCAt                                                                                                               <  1:206988/123‑1 (MQ=255)

                                    gCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCAt                                                                                                               >  2:206988/1‑123 (MQ=255)

                                     cGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAg                                                                                             <  1:273650/140‑1 (MQ=255)

                                                              cGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAAt                                                                    >  1:860645/1‑140 (MQ=255)

                                                                        cacaCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGAtt                                                          >  2:944337/1‑140 (MQ=255)

                                                                        cacaCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGAtt                                                          >  1:698653/1‑140 (MQ=255)

                                                                             ccGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGCATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGc                                                     >  1:456936/1‑140 (MQ=255)

                                                                               gccagcgccagcAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAg                                                   >  2:428098/1‑140 (MQ=255)

                                                                                    cgccagcAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACggg                                              <  1:944337/140‑1 (MQ=255)

                                                                                                 gACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTg                                 >  1:100262/1‑140 (MQ=255)

                                                                                                  aCGCCGGAACGTGATCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTgc                                >  2:16919/1‑140 (MQ=255)

                                                                                                   cGCCGGAACGTGATCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTgcg                               <  1:16919/140‑1 (MQ=255)

                                                                                                   cGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTgcg                               <  1:428098/140‑1 (MQ=255)

                                                                                                      cGGAACGTGCCCGTCGCAAATCCGGGAAACAATGCCAAAAGTATCAAATACGGTCATCGCGGTGAAATAGGCCGGACCCATCTTCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCgcag                            <  2:456936/140‑1 (MQ=255)

                                                                                                      cGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTg                                         >  2:514775/1‑127 (MQ=255)

                                                                                                      cGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTg                                         <  1:514775/127‑1 (MQ=255)

                                                                                                        gAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCgcagca                          <  1:841975/140‑1 (MQ=255)

                                                                                                             tGACCGGCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATAtt                     <  2:334500/140‑1 (MQ=255)

                                                                                                                   tCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATATTCAgtgg               >  1:545855/1‑140 (MQ=255)

                                                                                                                                agaCAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATATTCAGTGGTGAATTAATAGcc  >  2:514943/1‑140 (MQ=255)

CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATATTCAGTGGTGAATTAATAGCC  >  NC_000913/1358918‑1359185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A