Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,579,871	(T)_8→9	coding (920/1158 nt)	ydeM ←	putative anaerobic sulfatase maturation enzyme YdeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,579,863	1	.	T	95.3%	145.9 / ‑0.3	43	coding (928/1158 nt)	ydeM	putative anaerobic sulfatase maturation enzyme YdeM
Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (18/23); total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

CCATGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑TTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAT  >  NC_000913/1579725‑1579997

ccATGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑t                                                                                                                                        >  2:11070/1‑140 (MQ=255)

 cATGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tt                                                                                                                                       >  2:241038/1‑140 (MQ=255)

   tGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt                                                                                                                                     >  1:914665/1‑140 (MQ=255)

       tGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt‑tttt                                                                                                                                >  2:220946/1‑140 (MQ=255)

        gAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt‑ttttt                                                                                                                               >  2:464338/1‑139 (MQ=255)

        gAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt‑ttttt                                                                                                                               >  1:431063/1‑139 (MQ=255)

        gAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTCTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt‑ttttt                                                                                                                               >  1:152802/1‑139 (MQ=255)

               ggATTTTATACCCTTCGAAAAAATAAAAAACAGTCTCATTTTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGt                                                                                                                         <  2:172396/140‑1 (MQ=255)

               ggATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTTTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGt                                                                                                                         <  1:3973/140‑1 (MQ=255)

               ggATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGt                                                                                                                         <  1:12765/140‑1 (MQ=255)

                         cccTTCGAAAAAATAAAAAACAGTCTCATTGTTTATTTTAGTAATACGTTGTTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTaca                                                                                                               <  2:544438/139‑1 (MQ=255)

                          ccTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTacac                                                                                                              >  1:52016/1‑140 (MQ=255)

                             tCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGt                                                                                                           >  2:834552/1‑140 (MQ=255)

                              cGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGtt                                                                                                          >  1:436696/1‑140 (MQ=255)

                              cGCAAAAATAAGAAACAGTCTCATTGTTTAC‑TTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑TTTTCTTTTGCGCTGt                                                                                                                         >  1:521470/1‑124 (MQ=255)

                              cGCAAAAATAAGAAACAGTCTCATTGTTTAC‑TTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑TTTTCTTTTGCGCTGt                                                                                                                         <  2:521470/124‑1 (MQ=255)

                                       aaaaaaCAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGtttt                                                                                                 <  2:39982/137‑1 (MQ=255)

                                            aCAGTCTTTTTTTTTATTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGtt                                                                                            <  2:603981/140‑1 (MQ=255)

                                               ttctcTTTGTTTATTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAg                                                                                         <  2:152802/139‑1 (MQ=255)

                                               gTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAg                                                                                         >  2:775119/1‑140 (MQ=255)

                                                          tACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATg                                                                              >  1:531692/1‑140 (MQ=255)

                                                            ttttAGTAATACGATGCTTAGAACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGtt                                                                            <  2:28733/139‑1 (MQ=255)

                                                              ttAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTc                                                                          <  2:531692/140‑1 (MQ=255)

                                                                         aTGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTAc                                                               <  1:775119/140‑1 (MQ=255)

                                                                         aTGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTAc                                                               >  2:125476/1‑140 (MQ=255)

                                                                         aTGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGA‑TTATTAATGTTTCCAATTTTGTACt                                                              <  1:11070/140‑1 (MQ=255)

                                                                          tGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACt                                                              >  2:934601/1‑140 (MQ=255)

                                                                          tGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTACGCTGTCAGTTGTAAACTGTTCATCGTTTTTGGTTCAGTTTTTTTAATGTTTCAAATTTGGTACt                                                              >  2:196780/1‑140 (MQ=255)

                                                                                      ccGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACaa                                                  >  1:525824/1‑140 (MQ=255)

                                                                                      ccGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACaa                                                  >  2:345280/1‑140 (MQ=255)

                                                                                         ccATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAAt                                               >  2:661304/1‑140 (MQ=255)

                                                                                           aTTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACt                                                              >  1:891102/1‑123 (MQ=255)

                                                                                           aTTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACt                                                              <  2:891102/123‑1 (MQ=255)

                                                                                              gCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCg                                          <  1:14827/140‑1 (MQ=255)

                                                                                               cAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGc                                         <  1:220946/140‑1 (MQ=255)

                                                                                                 gATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCAt                                       <  1:934601/140‑1 (MQ=255)

                                                                                                    atgTTTATATGCGCCATGCTGACATTACGCTTTTATACGCATAT‑TTTTGCGATTTCAGTTGTATACTTTTCTTCTTTTTCAGTTAAGATTTATTAATGTTTCCAATTTTGTACTGTGTATAGAAAAAATGGCAGTATTCg                                    >  2:433211/3‑140 (MQ=255)

                                                                                                    aGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCg                                    >  2:563892/1‑140 (MQ=255)

                                                                                                                acaTTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCa                        <  1:345280/140‑1 (MQ=255)

                                                                                                                    tGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTAc                    >  2:821250/1‑140 (MQ=255)

                                                                                                                    tGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTAc                    >  2:153379/1‑140 (MQ=255)

                                                                                                                     gCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACt                   >  2:741224/1‑140 (MQ=255)

                                                                                                                          cATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTcaa              <  1:233426/140‑1 (MQ=255)

                                                                                                                          cATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTcaa              >  1:688407/1‑140 (MQ=255)

                                                                                                                             ttCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTcaacaa           <  1:661304/140‑1 (MQ=255)

                                                                                                                              tCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCaacaac          >  1:442296/1‑140 (MQ=255)

                                                                                                                              tCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCaacaac          <  1:433211/140‑1 (MQ=255)

                                                                                                                                  tGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGa      <  1:153379/140‑1 (MQ=255)

                                                                                                                                     aaTCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTa   >  1:919739/1‑140 (MQ=255)

                                                                                                                                      aTCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAt  <  1:741224/140‑1 (MQ=255)

                                                                                                                                      aTCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAt  <  1:834552/140‑1 (MQ=255)

                                                                                                                                      aTCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAt  <  2:919739/140‑1 (MQ=255)

CCATGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑TTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAT  >  NC_000913/1579725‑1579997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A