Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,732,446	T→C	G1120G (GGT→GGC)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,732,446	0	T	C	100.0%	190.6 / NA	53	G1120G (GGT→GGC)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/29); total (24/29)

GGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATCCTGCCTGCCC  >  NC_000913/1732311‑1732576

ggTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGcc                                                                                                                                >  2:710251/1‑140 (MQ=255)

   gAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCgg                                                                                                                             >  1:866474/1‑140 (MQ=255)

    aGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGgc                                                                                                                            >  1:595773/1‑140 (MQ=255)

     gCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCC‑CCACCGATGGCAGCCCGgcgc                                                                                                                          >  1:112579/1‑140 (MQ=255)

      cGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGgcgc                                                                                                                          >  1:681146/1‑140 (MQ=255)

      cGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGgcgc                                                                                                                          >  1:658916/1‑140 (MQ=255)

      cGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGgcgc                                                                                                                          >  1:755956/1‑140 (MQ=255)

        aTGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctc                                                                                                                        <  1:198321/140‑1 (MQ=255)

        aTGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctc                                                                                                                        <  1:121462/140‑1 (MQ=255)

        aTGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctc                                                                                                                        <  2:658916/140‑1 (MQ=255)

        aTGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctc                                                                                                                        <  2:681146/140‑1 (MQ=255)

          gAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCtt                                                                                                                      <  1:165204/140‑1 (MQ=255)

             gTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGc                                                                                                                   <  1:710251/140‑1 (MQ=255)

              tATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGGTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGcc                                                                                                                  <  2:496625/140‑1 (MQ=255)

                  tcgtcgTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCC‑CCACCGATGGCAGCCCGGCGCTCTTTGCCTCAAc                                                                                                             <  2:112579/140‑1 (MQ=255)

                   cgtcgtCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAAc                                                                                                             >  2:777525/1‑140 (MQ=255)

                     tcgtcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACAt                                                                                                           >  1:499017/1‑140 (MQ=255)

                        tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctct                                                                                                                       >  1:269595/1‑125 (MQ=255)

                        tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctct                                                                                                                       <  2:269595/125‑1 (MQ=255)

                        tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGc                                                                                                        >  1:668004/1‑140 (MQ=255)

                         cTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGcc                                                                                                       <  1:460851/140‑1 (MQ=255)

                         cTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGcc                                                                                                       <  2:668004/140‑1 (MQ=255)

                         cTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGcc                                                                                                       <  2:138765/140‑1 (MQ=255)

                                 gcgcTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGGGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGttt                                                                                               >  1:437899/1‑140 (MQ=255)

                                    ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                                            <  2:947305/140‑1 (MQ=255)

                                    ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                                            <  2:499017/140‑1 (MQ=255)

                                    ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                                            <  2:755956/140‑1 (MQ=255)

                                    ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                                            <  1:777525/140‑1 (MQ=255)

                                             aGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGGGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAg                                                                                   <  2:437899/140‑1 (MQ=255)

                                              gccgccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGa                                                                                  >  2:600284/1‑140 (MQ=255)

                                                 gccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATgg                                                                               <  2:899402/140‑1 (MQ=255)

                                                 gccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATgg                                                                               >  2:649391/1‑140 (MQ=255)

                                                   cAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCg                                                                             <  1:584676/140‑1 (MQ=255)

                                                     gagaAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTg                                                                           <  2:124832/140‑1 (MQ=255)

                                                       gaAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGAt                                                                         <  1:649391/140‑1 (MQ=255)

                                                        aaGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATg                                                                        <  1:311690/140‑1 (MQ=255)

                                                          gCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCg                                                                             >  1:861577/1‑133 (MQ=255)

                                                          gCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCg                                                                             <  2:861577/133‑1 (MQ=255)

                                                                cgTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGAt                                                                >  2:754043/1‑140 (MQ=255)

                                                                        tGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGc                                                        <  2:866474/140‑1 (MQ=255)

                                                                                cGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGa                                                <  1:807557/140‑1 (MQ=255)

                                                                                cGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGa                                                <  2:23179/140‑1 (MQ=255)

                                                                                   ccTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTCCCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTc                                             <  1:6968/140‑1 (MQ=255)

                                                                                       tGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGtt                                         >  1:686631/1‑140 (MQ=255)

                                                                                         cgcgATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAg                                               >  2:495177/1‑132 (MQ=255)

                                                                                         cgcgATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAg                                               <  1:495177/132‑1 (MQ=255)

                                                                                             aTTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCg                                   >  1:456108/1‑140 (MQ=255)

                                                                                             aTTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCg                                   >  1:318202/1‑140 (MQ=255)

                                                                                                gctgctGGAACGTCGGGGCGTATTCCCCGCCCCCGATGGCAGCCCGGCGCTTTTTGCCTCAACACCGCCGGGCGTTTATGGGGGCGTAGAGGGCGTGAGGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCt                                <  2:318202/140‑1 (MQ=255)

                                                                                                    ggggACCGTCGGGGGTTATTCCCCGCCCCCGAGGGCAGCCCGGCGCTTTTTCCCCAAACACCGCCGGGGGTTTATGGGGGGGTAGATGGGGTGAGGCGGGTGATCGAACAGCTTGCGGGAGTCGTTTTACCCGCCTCAct                            <  2:456108/138‑1 (MQ=255)

                                                                                                              aGGGCGTATTCCCCGCCACCGATGGCAGCCGGGCGCTCTTTGCCTCAACACCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGc                  <  1:512197/140‑1 (MQ=255)

                                                                                                                    ttttccccGCCACCGAGGGCAGCCGGGCGCTTTTTGCCTCAACATCGCCGGGCGTTTAGGGGGGCGTAGAGGGGGTGAGGGGGGTGATCGAACAGCTGGCGGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATc            <  2:686631/135‑1 (MQ=255)

                                                                                                                     aTTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATcc           >  1:747976/1‑140 (MQ=255)

                                                                                                                           cGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATcctgcctg     >  1:165742/1‑140 (MQ=255)

                                                                                                                             ccACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATCCtgcctgcc   >  2:333262/1‑140 (MQ=255)

                                                                                                                              ccccGAGGGCAGCCGGGCGCTTTTTGCCTCAACACCGCCGGGCGTTTATGAGGGCGTAGAGGGCGTGAGGCGGGTGATCGAACAGCTGGCCGGGGTCGGTTTCCCCGCCTCACTCTGGGAAAGCCAGATCCTGCCTGccc  <  2:110699/138‑1 (MQ=255)

                                                                                                                              cACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATCCTGCCTGccc  >  1:110699/1‑140 (MQ=255)

GGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATCCTGCCTGCCC  >  NC_000913/1732311‑1732576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A