Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F28 I1 R1
|
120 |
56.0 |
1898522 |
99.4% |
1887130 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,641,940 |
G→A |
A302V (GCC→GTC) |
rodZ ← |
transmembrane component of cytoskeleton |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,641,940 | 0 | G | A | 100.0%
| 88.9
/ NA
| 26 | A302V (GCC→GTC) | rodZ | transmembrane component of cytoskeleton |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/12); total (14/12) |
TACTGCGCCGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGGCTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTT > NC_000913/2641832‑2642073
|
tACTGCGCCGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGc < 1:719388/140‑1 (MQ=255)
tACTGCGCCGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGc < 1:794252/140‑1 (MQ=255)
ccGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCg > 2:312490/1‑140 (MQ=255)
cGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCgg < 1:694876/140‑1 (MQ=255)
tGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGATa > 2:414579/1‑140 (MQ=255)
ggCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAAt > 1:715086/1‑140 (MQ=255)
aTTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTAc < 2:715086/140‑1 (MQ=255)
gTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCttt < 2:514015/140‑1 (MQ=255)
cAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACg < 1:394082/133‑1 (MQ=255)
cAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACg > 2:394082/1‑133 (MQ=255)
tCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAAtt > 1:563832/1‑140 (MQ=255)
gATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTaaa < 1:757189/132‑1 (MQ=255)
gATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTaaa > 2:453991/1‑132 (MQ=255)
gATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTaaa > 2:757189/1‑132 (MQ=255)
gATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTaaa < 1:453991/132‑1 (MQ=255)
aTCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAg > 2:579162/1‑140 (MQ=255)
ttCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTccag > 1:676946/1‑140 (MQ=255)
aTACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAAt > 1:408577/1‑140 (MQ=255)
tGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGc > 2:267046/1‑140 (MQ=255)
gATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAg > 1:12307/1‑140 (MQ=255)
tCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTa < 2:12307/140‑1 (MQ=255)
gTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTGCCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAg > 2:884470/1‑140 (MQ=255)
gTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAg > 2:780081/1‑140 (MQ=255)
aCTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGtt < 1:780081/140‑1 (MQ=255)
aCTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGtt < 1:579162/140‑1 (MQ=255)
aCTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGtt < 2:88081/140‑1 (MQ=255)
|
TACTGCGCCGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGGCTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTT > NC_000913/2641832‑2642073
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
