Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,026,670	A→G	N302S (AAC→AGC)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,026,670	0	A	G	100.0%	102.6 / NA	29	N302S (AAC→AGC)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/11); total (18/11)

TTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAA  >  NC_000913/3026536‑3026799

ttATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCttt                                                                                                                              >  1:702775/1‑140 (MQ=255)

             tACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCgg                                                                                                                 >  1:574142/1‑140 (MQ=255)

                      tACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCaa                                                                                                        >  1:173232/1‑140 (MQ=255)

                      tACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCaa                                                                                                        >  2:827682/1‑140 (MQ=255)

                            cTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGaa                                                                                                  >  1:595537/1‑140 (MQ=255)

                            cTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTCTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGaa                                                                                                  <  2:652921/140‑1 (MQ=255)

                              gACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGaaaa                                                                                                >  2:780395/1‑140 (MQ=255)

                              gACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGaaaa                                                                                                >  1:810597/1‑140 (MQ=255)

                                ccGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAAc                                                                                                       <  2:846736/131‑1 (MQ=255)

                                ccGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAAc                                                                                                       >  1:846736/1‑131 (MQ=255)

                                   gTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCa                                                                                           <  2:574142/140‑1 (MQ=255)

                                      aaaaCTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATgg                                                                                        <  2:173232/140‑1 (MQ=255)

                                                           gCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGc                                                                   >  2:206156/1‑140 (MQ=255)

                                                              tCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATg                                                                >  2:246683/1‑140 (MQ=255)

                                                              tCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATg                                                                >  2:875699/1‑140 (MQ=255)

                                                               ccATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCTGAAGAAAGTTAAAGTGGGCATgg                                                               >  1:731442/1‑140 (MQ=255)

                                                               ccATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATgg                                                               >  1:454387/1‑140 (MQ=255)

                                                                      gaagaaAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGa                                                        >  1:553461/1‑140 (MQ=255)

                                                                                          tctcAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACtt                                    >  2:703010/1‑140 (MQ=255)

                                                                                                   aaCCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGaa                                          <  2:421763/125‑1 (MQ=255)

                                                                                                   aaCCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGaa                                          >  1:421763/1‑125 (MQ=255)

                                                                                                   aaCCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACAtgc                           >  2:244909/1‑140 (MQ=255)

                                                                                                      cAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACAtgctgc                        <  2:86931/140‑1 (MQ=255)

                                                                                                                    cTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGaa          <  1:206156/140‑1 (MQ=255)

                                                                                                                           tCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCTGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa   <  2:731442/140‑1 (MQ=255)

                                                                                                                           tCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa   <  1:875699/140‑1 (MQ=255)

                                                                                                                           tCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa   <  1:726253/140‑1 (MQ=255)

                                                                                                                            ccGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACaa  >  2:906537/1‑140 (MQ=255)

                                                                                                                                   ccAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa   <  1:193389/132‑1 (MQ=255)

TTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAA  >  NC_000913/3026536‑3026799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A