Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 120 56.0 1898522 99.4% 1887130 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,393,494 T→C N922S (AAC→AGC)  yhdP ← AsmA2 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,393,4940TC100.0% 210.2 / NA 58N922S (AAC→AGC) yhdPAsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/31);  total (27/31)

ATTTGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGTTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGG  >  NC_000913/3393378‑3393630
                                                                                                                    |                                                                                                                                        
atTTGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGt                                                                                                                   >  1:97965/1‑140 (MQ=255)
atTTGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGt                                                                                                                   <  1:199288/140‑1 (MQ=255)
  ttGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGa                                                                                                                 <  1:707685/140‑1 (MQ=255)
     atatTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGTCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGacaa                                                                                                              >  1:811689/1‑140 (MQ=255)
     atatTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGacaa                                                                                                              >  1:59471/1‑140 (MQ=255)
       atTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGacaaca                                                                                                            <  2:899997/140‑1 (MQ=255)
        ttCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACAt                                                                                                           >  1:139892/1‑140 (MQ=255)
          cGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAg                                                                                                         <  1:818297/140‑1 (MQ=255)
          cGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAg                                                                                                         <  1:772745/140‑1 (MQ=255)
           gccagccaCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAgg                                                                                                        <  1:183840/140‑1 (MQ=255)
           gccagccaCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAgg                                                                                                        >  2:471201/1‑140 (MQ=255)
            ccagccaCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGc                                                                                                       <  2:59471/140‑1 (MQ=255)
                 caCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGcgtacg                                                                                                  <  1:601545/140‑1 (MQ=255)
                  aCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGcgtacgt                                                                                                 <  1:121113/140‑1 (MQ=255)
                   cGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGcgtacgta                                                                                                <  2:139892/140‑1 (MQ=255)
                          ttattaCGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTAt                                                                                         <  1:471201/140‑1 (MQ=255)
                                  cATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTtgtg                                                                                 >  2:665565/1‑140 (MQ=255)
                                    tCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTggg                                                                               >  1:471700/1‑140 (MQ=255)
                                     cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACg                                                                                             >  1:803592/1‑125 (MQ=255)
                                     cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACg                                                                                             <  2:803592/125‑1 (MQ=255)
                                     cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGa                                                                              >  1:625728/1‑140 (MQ=255)
                                     cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGa                                                                              >  2:546039/1‑140 (MQ=255)
                                     cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGa                                                                              >  2:483896/1‑140 (MQ=255)
                                     cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGa                                                                              >  2:348333/1‑140 (MQ=255)
                                       cTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGaaa                                                                            >  2:7651/1‑140 (MQ=255)
                                           cGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCg                                                                        <  1:30153/140‑1 (MQ=255)
                                            gTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTAt                                                                                         >  1:89139/1‑122 (MQ=255)
                                            gTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTAt                                                                                         <  2:89139/122‑1 (MQ=255)
                                             tGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCt                                                                      <  1:437045/140‑1 (MQ=255)
                                              ggCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTg                                                                     <  2:472806/140‑1 (MQ=255)
                                               gCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGTGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGc                                                                    >  1:229795/1‑140 (MQ=255)
                                               gCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGc                                                                    >  2:936595/1‑140 (MQ=255)
                                                cGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGca                                                                   >  2:100561/1‑140 (MQ=255)
                                                 gTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGcac                                                                  <  2:97965/140‑1 (MQ=255)
                                                 gTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGcac                                                                  <  1:259769/140‑1 (MQ=255)
                                                  ttGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGcacc                                                                 >  1:640849/1‑140 (MQ=255)
                                                   tGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGcacca                                                                <  2:625728/140‑1 (MQ=255)
                                                      tttCACGCCCTTGCGCTTCAACCAGGGTGTCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCg                                                             <  2:811689/140‑1 (MQ=255)
                                                                 tGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCg                                                             <  1:703951/129‑1 (MQ=255)
                                                                 tGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCg                                                             >  2:703951/1‑129 (MQ=255)
                                                                      ttCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             <  2:944502/140‑1 (MQ=255)
                                                                      ttCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             <  2:230865/140‑1 (MQ=255)
                                                                        cAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             >  1:155317/1‑138 (MQ=255)
                                                                        cAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             <  2:155317/138‑1 (MQ=255)
                                                                        cAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCt                                           <  2:471700/140‑1 (MQ=255)
                                                                          aCCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCttt                                         >  2:245638/1‑140 (MQ=255)
                                                                              gggTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             >  2:475154/1‑132 (MQ=255)
                                                                              gggTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             <  1:475154/132‑1 (MQ=255)
                                                                                gTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGaa                                   >  1:258749/1‑140 (MQ=255)
                                                                                gTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGaa                                   <  1:546039/140‑1 (MQ=255)
                                                                                        tGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGgcca                           >  1:871056/1‑140 (MQ=255)
                                                                                          ccGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGgccagc                         >  2:35706/1‑140 (MQ=255)
                                                                                              cGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACt                     <  1:816244/140‑1 (MQ=255)
                                                                                                   gCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCa                <  1:7651/140‑1 (MQ=255)
                                                                                                           aTACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCAt        >  2:13371/1‑140 (MQ=255)
                                                                                                             aCTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATcg      <  1:665565/140‑1 (MQ=255)
                                                                                                                cAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGTGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATcggcg   <  2:229795/140‑1 (MQ=255)
                                                                                                                 aGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATcggcgg  >  2:384919/1‑140 (MQ=255)
                                                                                                                    |                                                                                                                                        
ATTTGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGTTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGG  >  NC_000913/3393378‑3393630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A