Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 120 56.0 1898522 99.4% 1887130 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,441,981 T→C N40D (AAC→GAC)  rpsK ← 30S ribosomal subunit protein S11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,441,9810TC100.0% 173.9 / NA 49N40D (AAC→GAC) rpsK30S ribosomal subunit protein S11
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/19);  total (30/19)

ATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCACCCCGAT  >  NC_000913/3441845‑3442115
                                                                                                                                        |                                                                                                                                      
aTAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCAcc                                                                                                                                     >  2:470373/1‑140 (MQ=255)
  aaCTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCt                                                                                                                                   >  2:460327/1‑140 (MQ=255)
  aaCTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCt                                                                                                                                   >  2:785377/1‑140 (MQ=255)
    cTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCGCCCTGa                                                                                                                                 <  2:529084/140‑1 (MQ=255)
    cTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGa                                                                                                                                 >  2:134955/1‑140 (MQ=255)
    cTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGa                                                                                                                                 <  2:799389/140‑1 (MQ=255)
      tCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACg                                                                                                                               <  1:470373/140‑1 (MQ=255)
       ccAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGa                                                                                                                              >  2:689887/1‑140 (MQ=255)
        cAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGAt                                                                                                                             <  1:681819/140‑1 (MQ=255)
        cAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGAt                                                                                                                             >  2:478933/1‑140 (MQ=255)
             tCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTg                                                                                                                        <  1:686385/140‑1 (MQ=255)
               ttGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGAt                                                                                                                      <  1:134955/140‑1 (MQ=255)
                  aTGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGt                                                                                                                   <  1:568096/140‑1 (MQ=255)
                     ccGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCAc                                                                                                                >  2:95930/1‑140 (MQ=255)
                       gTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGa                                                                                                              >  2:803407/1‑140 (MQ=255)
                         aTTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATg                                                                                                            <  1:478933/140‑1 (MQ=255)
                         aTTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATg                                                                                                            <  1:95930/140‑1 (MQ=255)
                            cTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGgtg                                                                                                         <  1:689887/140‑1 (MQ=255)
                                  cGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGttgttg                                                                                                   >  2:646404/1‑140 (MQ=255)
                                  cGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGttgttg                                                                                                   >  2:884034/1‑140 (MQ=255)
                                  cGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGttgttg                                                                                                   >  2:256969/1‑140 (MQ=255)
                                   ggCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGa                                                                                                  >  1:16520/1‑140 (MQ=255)
                                    gCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGaa                                                                                                 >  1:907060/1‑140 (MQ=255)
                                     cGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGaaa                                                                                                >  1:136814/1‑140 (MQ=255)
                                       tCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAaaga                                                                                              >  1:247796/1‑140 (MQ=255)
                                       tCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAaaga                                                                                              >  1:462072/1‑140 (MQ=255)
                                           cgcAACGCTCAGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCa                                                                                          <  1:884034/140‑1 (MQ=255)
                                               aCGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGa                                                                                      >  2:811578/1‑140 (MQ=255)
                                                  ctctGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGatat                                                                                   <  2:427379/140‑1 (MQ=255)
                                                         gcAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCAc                                                                            <  2:907060/140‑1 (MQ=255)
                                                          cAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACg                                                                           <  1:460327/140‑1 (MQ=255)
                                                                 aGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCag                                                                    >  1:824142/1‑140 (MQ=255)
                                                                  gCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCaga                                                                   >  1:731976/1‑140 (MQ=255)
                                                                   cTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCagag                                                                  <  2:731976/140‑1 (MQ=255)
                                                                      cAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACt                                                               >  2:402981/1‑140 (MQ=255)
                                                                      cAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACt                                                               >  2:299142/1‑140 (MQ=255)
                                                                                       cGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGttt                                              >  1:790902/1‑140 (MQ=255)
                                                                                            aCCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTg                                         >  2:789601/1‑140 (MQ=255)
                                                                                                       cGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGt                              >  2:913655/1‑140 (MQ=255)
                                                                                                           accaccGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCt                          >  2:278947/1‑140 (MQ=255)
                                                                                                            ccaccGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCtt                         <  1:811578/140‑1 (MQ=255)
                                                                                                                cGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGcc                     <  1:803407/140‑1 (MQ=255)
                                                                                                                cGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGcc                     <  2:462072/140‑1 (MQ=255)
                                                                                                                 ggCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCa                    >  1:711806/1‑140 (MQ=255)
                                                                                                                 ggCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCa                    >  1:488872/1‑140 (MQ=255)
                                                                                                                   cTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCatt                  >  2:565156/1‑140 (MQ=255)
                                                                                                                             acccaacGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCAc        >  2:87388/1‑140 (MQ=255)
                                                                                                                              cccaacGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCAcc       <  1:913655/140‑1 (MQ=255)
                                                                                                                                   cgcgTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGGTATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCACCCCGat  >  2:467174/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCACCCCGAT  >  NC_000913/3441845‑3442115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A