Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	106	90.3	3060316	99.2%	3035833	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,522,012	A→G	F352L (TTC→CTC)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,522,012	0	A	G	98.7%	271.7 / ‑3.3	74	F352L (TTC→CTC)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (29/44); minor base C (0/1); total (29/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

ATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAACGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGACTGGTACTGG  >  NC_000913/1521876‑1522145

aTGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGCAGTTATTGTAGCCCTTGCGGCTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGt                                                                                                                                    >  2:68611/1‑140 (MQ=255)

 tGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTg                                                                                                                                   >  2:1246995/1‑140 (MQ=255)

        caTCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGaa                                                                                                                            >  1:514866/1‑140 (MQ=255)

          tCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACg                                                                                                                          <  1:919983/140‑1 (MQ=255)

           cAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGc                                                                                                                         >  1:978492/1‑140 (MQ=255)

            aGATTGCGTTCGTCGCGACGCAACTCGCCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGcc                                                                                                                        <  2:1184589/140‑1 (MQ=255)

            aGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGcc                                                                                                                        <  1:1060587/140‑1 (MQ=255)

            aGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGcc                                                                                                                        <  2:305915/140‑1 (MQ=255)

            aGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGcc                                                                                                                        <  2:1209447/140‑1 (MQ=255)

            aGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGcc                                                                                                                        <  1:1214196/140‑1 (MQ=255)

              aTTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCa                                                                                                                      >  1:900108/1‑140 (MQ=255)

              aTTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCa                                                                                                                      >  1:1121244/1‑140 (MQ=255)

              aTTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCa                                                                                                                      <  2:913115/140‑1 (MQ=255)

              aTTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGACATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCa                                                                                                                      <  2:873782/140‑1 (MQ=255)

                   gTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTca                                                                                                                 >  1:1473204/1‑140 (MQ=255)

                     tcgtcgCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCGGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTcacc                                                                                                               <  1:1215975/140‑1 (MQ=255)

                     tcgtcgCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTcacc                                                                                                               <  1:109467/140‑1 (MQ=255)

                     tcgtcgCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTcacc                                                                                                               <  2:530665/140‑1 (MQ=255)

                     tcgtcgCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACACATGTTTTCGTAGTTCAGGCCGGTAGTGACCGTGACCGGAACGCCCAGTTcacc                                                                                                               <  2:1120445/140‑1 (MQ=255)

                     tcgccgCGACGCAACTCACCTTTTTGCCCGTTCTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTcacc                                                                                                               <  2:940111/140‑1 (MQ=255)

                       gtcgCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTcaccac                                                                                                             >  1:117488/1‑140 (MQ=255)

                       gtcgCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTcaccac                                                                                                             >  1:317756/1‑140 (MQ=255)

                       gtcgCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCACGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTcaccac                                                                                                             >  1:1135051/1‑140 (MQ=255)

                                  aCTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAg                                                                                                  <  1:1014148/140‑1 (MQ=255)

                                    tCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCg                                                                                                <  2:1522681/140‑1 (MQ=255)

                                     cACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCgg                                                                                               <  1:765291/140‑1 (MQ=255)

                                     cACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCgg                                                                                               <  2:297550/140‑1 (MQ=255)

                                      cccTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGc                                                                                              <  2:1306340/139‑1 (MQ=255)

                                       ccTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCt                                                                                             <  2:1219679/140‑1 (MQ=255)

                                        cTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGCGTGTCCAGCGGCTg                                                                                            >  1:1518009/1‑140 (MQ=255)

                                            ttGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTa                                                                                        <  2:401185/140‑1 (MQ=255)

                                             tGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTAt                                                                                       <  1:1301016/140‑1 (MQ=255)

                                                ccGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTcc                                                                                    <  2:42420/140‑1 (MQ=255)

                                                 cGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTccc                                                                                   <  2:923082/140‑1 (MQ=255)

                                                  gTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCt                                                                                  >  2:66304/1‑140 (MQ=255)

                                                         ggCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATg                                                                           <  1:1246995/140‑1 (MQ=255)

                                                         ggCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATg                                                                           >  1:698159/1‑140 (MQ=255)

                                                             tGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGt                                                                       <  1:87857/140‑1 (MQ=255)

                                                             tGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGt                                                                       >  2:961738/1‑140 (MQ=255)

                                                              gCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGtt                                                                      >  2:546207/1‑140 (MQ=255)

                                                               ccGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTg                                                                     <  1:229717/140‑1 (MQ=255)

                                                                cGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGc                                                                    >  2:568645/1‑140 (MQ=255)

                                                                 gCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCa                                                                   <  1:793030/140‑1 (MQ=255)

                                                                  cTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAg                                                                  <  2:17290/140‑1 (MQ=255)

                                                                               aGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCAcgccg                                                     >  1:538182/1‑140 (MQ=255)

                                                                               aGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCAcgccg                                                     >  1:254790/1‑140 (MQ=255)

                                                                                     tGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCa                                               <  1:134512/140‑1 (MQ=255)

                                                                                       tAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCAtg                                             >  1:1279381/1‑140 (MQ=255)

                                                                                          cccTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGa                                          >  1:1478864/1‑140 (MQ=255)

                                                                                            cTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAACGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACg                                        >  2:365519/1‑140 (MQ=255)

                                                                                              tGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACggg                                      <  2:117488/140‑1 (MQ=255)

                                                                                              tGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACggg                                      <  1:521076/140‑1 (MQ=255)

                                                                                              tGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACggg                                      >  2:37362/1‑140 (MQ=255)

                                                                                              tGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACggg                                      >  1:747007/1‑140 (MQ=255)

                                                                                              tGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACggg                                      >  1:639138/1‑140 (MQ=255)

                                                                                               gCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCATGCCGCCCGCATGTGACgggg                                     >  2:894945/1‑139 (MQ=255)

                                                                                                   ttttCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAg                                 <  2:408663/140‑1 (MQ=255)

                                                                                                          ccATGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCCCGCCGCCCGCATGTGACGGGTTAAGTTgtggt                          <  2:953758/139‑1 (MQ=255)

                                                                                                             tGTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCTGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGcc                       <  1:280997/140‑1 (MQ=255)

                                                                                                              gTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCa                      <  1:703740/140‑1 (MQ=255)

                                                                                                              gTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCa                      <  2:660754/140‑1 (MQ=255)

                                                                                                              gTTTTCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCa                      <  2:627145/140‑1 (MQ=255)

                                                                                                              gTTTTCGTAGTACAGGCCGTTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCa                      <  2:98697/140‑1 (MQ=255)

                                                                                                               ttttCGTAGTTCAGGCCGGTCGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAAGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCAt                     <  2:784823/140‑1 (MQ=255)

                                                                                                               ttttCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCAt                     >  2:940961/1‑140 (MQ=255)

                                                                                                                tttCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGCCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATg                    <  2:1518009/140‑1 (MQ=255)

                                                                                                                 ttCGTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCCGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGCAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATgg                   >  1:889422/1‑140 (MQ=255)

                                                                                                                    gTAGTTCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTa                <  1:568645/140‑1 (MQ=255)

                                                                                                                        ttCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGGTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGa            <  2:889422/140‑1 (MQ=255)

                                                                                                                        tgcAGGCCGGTAGTGAGCGTGACCGGAGCGCCCAGTTCGCCACGGTGTGTCCAGCGGCTGTCTATGCCCTGGGAGTGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGCCGGGTTAAGTTGTGGTGCCATGGGTATTGa            <  2:698159/138‑1 (MQ=255)

                                                                                                                         tCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGAc           <  1:575580/140‑1 (MQ=255)

                                                                                                                         tCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGAc           <  1:546207/140‑1 (MQ=255)

                                                                                                                         tCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGAc           <  1:361721/140‑1 (MQ=255)

                                                                                                                         tCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGAc           <  2:101943/140‑1 (MQ=255)

                                                                                                                         tCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGAc           <  2:1509434/140‑1 (MQ=255)

                                                                                                                         tCAGGCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGAc           <  2:1416449/140‑1 (MQ=255)

                                                                                                                             gCCGGTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGACTGGt       >  1:1446264/1‑140 (MQ=255)

                                                                                                                                ggTAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGACTGGTACt    <  1:68611/140‑1 (MQ=255)

                                                                                                                                  tAGTGAGCGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGACTGGTACTgg  >  1:1079678/1‑140 (MQ=255)

ATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTATTGTAGCCCTTGCGGTTTTCACTCATGTTTTCGTAGTTCAGGCCGGTAGTGAACGTGACCGGAACGCCCAGTTCACCACGGTGTGTCCAGCGGCTGTCTATTCCCTGGTAATGGCGTTGCAGGGTAATCACGCCGCCCGCATGTGACGGGTTAAGTTGTGGTGCCATGGGTATTGACTGGTACTGG  >  NC_000913/1521876‑1522145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A