Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 106 90.3 3060316 99.2% 3035833 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,938,454 T→C L269S (TTG→TCG)  pykA → pyruvate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,938,4540TC98.9% 329.3 / ‑4.0 90L269S (TTG→TCG) pykApyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (35/54);  minor base A (0/1);  total (35/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

AACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACG  >  NC_000913/1938322‑1938588
                                                                                                                                    |                                                                                                                                      
aaCGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCcgt                                                                                                                                 >  2:1469938/1‑140 (MQ=255)
  cGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCcgtcg                                                                                                                               <  1:303223/140‑1 (MQ=255)
      cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTgcg                                                                                                                           >  1:115191/1‑140 (MQ=255)
      cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTgcg                                                                                                                           >  2:969127/1‑140 (MQ=255)
         aaGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGt                                                                                                                        <  2:1307347/140‑1 (MQ=255)
         aaGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGt                                                                                                                        <  2:924290/140‑1 (MQ=255)
           gCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCa                                                                                                                      >  1:215564/1‑140 (MQ=255)
            ccGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAg                                                                                                                     <  1:1112549/140‑1 (MQ=255)
              gTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCt                                                                                                                   <  1:1271446/140‑1 (MQ=255)
               tttGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTa                                                                                                                  >  1:1125036/1‑140 (MQ=255)
                  gCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAAc                                                                                                               >  1:1203253/1‑140 (MQ=255)
                  gCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAAc                                                                                                               <  2:641982/140‑1 (MQ=255)
                     gCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGa                                                                                                            >  2:717282/1‑140 (MQ=255)
                     gCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGa                                                                                                            <  2:259121/140‑1 (MQ=255)
                        aGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGACATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCg                                                                                                         <  2:695770/140‑1 (MQ=255)
                                 tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                                                                                <  1:1293332/140‑1 (MQ=255)
                                 tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                                                                                <  1:146989/140‑1 (MQ=255)
                                 tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                                                                                <  1:786215/140‑1 (MQ=255)
                                  ggATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACgg                                                                                               <  1:755863/140‑1 (MQ=255)
                                  ggATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACgg                                                                                               <  1:256237/140‑1 (MQ=255)
                                    aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                                                                             >  1:1066939/1‑140 (MQ=255)
                                    aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                                                                             <  2:1343747/140‑1 (MQ=255)
                                    aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                                                                             <  2:438825/140‑1 (MQ=255)
                                     tGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGa                                                                                            <  2:1408287/140‑1 (MQ=255)
                                      gACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAc                                                                                           <  1:1169442/140‑1 (MQ=255)
                                      gACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAAACACGGCGAc                                                                                           <  2:762686/140‑1 (MQ=255)
                                        catcatCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                                                                         <  2:530728/140‑1 (MQ=255)
                                        catcatCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                                                                         <  1:1469938/140‑1 (MQ=255)
                                         atcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCa                                                                                        >  1:1028282/1‑140 (MQ=255)
                                         atcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCa                                                                                        >  2:523291/1‑140 (MQ=255)
                                           catcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAga                                                                                      >  2:350947/1‑140 (MQ=255)
                                                tCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGatgatg                                                                                 <  1:18508/140‑1 (MQ=255)
                                                tCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGatgatg                                                                                 <  1:82126/140‑1 (MQ=255)
                                                 cGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATgg                                                                                >  2:916362/1‑140 (MQ=255)
                                                   ccTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                                                                              <  1:211137/140‑1 (MQ=255)
                                                   ccTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                                                                              >  2:211137/1‑140 (MQ=255)
                                                     tctGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTc                                                                            <  1:1347344/140‑1 (MQ=255)
                                                        gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                                                         <  2:1435560/140‑1 (MQ=255)
                                                        gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                                                         <  1:1527012/140‑1 (MQ=255)
                                                        gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                                                         <  2:792157/140‑1 (MQ=255)
                                                        gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTAAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                                                         <  2:876692/140‑1 (MQ=255)
                                                         aCGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATg                                                                        <  2:1038613/140‑1 (MQ=255)
                                                            tGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGAtt                                                                     >  2:257020/1‑140 (MQ=255)
                                                              gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                                                                   >  1:819199/1‑140 (MQ=255)
                                                              gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                                                                   <  1:969127/140‑1 (MQ=255)
                                                                aaTGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCTGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTa                                                                 >  2:253191/1‑140 (MQ=255)
                                                                 aTGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTaa                                                                >  2:385023/1‑140 (MQ=255)
                                                                 aTGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTaa                                                                >  2:373205/1‑140 (MQ=255)
                                                                   ggTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAcg                                                              <  1:410971/140‑2 (MQ=255)
                                                                   ggTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAcc                                                              >  2:30791/1‑140 (MQ=255)
                                                                      tGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGa                                                           >  2:574049/1‑140 (MQ=255)
                                                                      tGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGa                                                           >  1:1371190/1‑140 (MQ=255)
                                                                       gCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCTCGGCGACCCAGATGATGGAGTCAATGAt                                                                      <  1:1195728/128‑1 (MQ=255)
                                                                       gCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGAt                                                                      >  2:1195728/1‑128 (MQ=255)
                                                                        cACGTGGCGACCTCGGTGTGGAAATTGGCGACGCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  2:929919/1‑140 (MQ=255)
                                                                        cACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  2:918898/1‑140 (MQ=255)
                                                                        cACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  2:119243/1‑140 (MQ=255)
                                                                        cACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  1:1253810/1‑140 (MQ=255)
                                                                        cACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  1:43748/1‑140 (MQ=255)
                                                                         aCGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGc                                                        >  1:1090727/1‑140 (MQ=255)
                                                                          cGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGcc                                                       <  2:841553/140‑1 (MQ=255)
                                                                          cGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGcc                                                       <  1:717282/140‑1 (MQ=255)
                                                                             ggCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAc                                                    >  1:581232/1‑140 (MQ=255)
                                                                                  ccTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTg                                               <  2:821767/140‑1 (MQ=255)
                                                                                     cGGTGTGGAAATTGGCGACCCGGACCTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAg                                            <  2:1125036/140‑1 (MQ=255)
                                                                                                 tGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCTCGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACg                                <  2:215564/140‑1 (MQ=255)
                                                                                                  ggCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGt                               >  1:219607/1‑140 (MQ=255)
                                                                                                          ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:1371190/140‑1 (MQ=255)
                                                                                                          ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:1354866/140‑1 (MQ=255)
                                                                                                          ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:1090727/140‑1 (MQ=255)
                                                                                                          ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:386145/140‑1 (MQ=255)
                                                                                                          ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:161583/140‑1 (MQ=255)
                                                                                                          ggAACTGGTCGGCATTCAGAAAGCGTAGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:992959/140‑1 (MQ=255)
                                                                                                           gAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGc                      >  2:573188/1‑140 (MQ=255)
                                                                                                               tGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAGACCGAGCGGTAATCACGGCGACCCAGATTATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGtt                  <  2:323616/140‑1 (MQ=255)
                                                                                                               tGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGtt                  <  1:257020/140‑1 (MQ=255)
                                                                                                               tGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGtt                  <  2:442372/140‑1 (MQ=255)
                                                                                                               tGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGtt                  <  2:794970/140‑1 (MQ=255)
                                                                                                                 gTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCt                >  1:1475553/1‑140 (MQ=255)
                                                                                                                 gTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCt                >  1:414927/1‑140 (MQ=255)
                                                                                                                  tCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTg               >  2:1381246/1‑140 (MQ=255)
                                                                                                                    gggaTTCAGAAAGCGTCGGTCCGTCGTGCGCGTCAGCTAAACCGGGGGGTAATCCCGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  2:1046261/137‑1 (MQ=255)
                                                                                                                    ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGTCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  2:501067/140‑1 (MQ=255)
                                                                                                                    ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  1:523291/140‑1 (MQ=255)
                                                                                                                    ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  1:397636/140‑1 (MQ=255)
                                                                                                                    ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  1:385023/140‑1 (MQ=255)
                                                                                                                    ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  1:286882/140‑1 (MQ=255)
                                                                                                                     gCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGAt            <  2:819199/140‑1 (MQ=255)
                                                                                                                     gCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGAt            <  2:85788/140‑1 (MQ=255)
                                                                                                                     gCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGAt            >  2:609947/1‑140 (MQ=255)
                                                                                                                             aaaGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGa    <  1:916362/140‑1 (MQ=255)
                                                                                                                               aGCGTCGATCCGGCGTGCGCGTCAGCTAAACCGGGCGGTAATCACGGCGACCCAGATGATGGTGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACg  <  2:1221884/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
AACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACG  >  NC_000913/1938322‑1938588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A