Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,074,478	A→G	A79A (GCA→GCG)	rutR →	DNA‑binding transcriptional dual regulator RutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,074,478	0	A	G	100.0%	172.2 / NA	46	A79A (GCA→GCG)	rutR	DNA‑binding transcriptional dual regulator RutR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/22); total (24/22)

TTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCG  >  NC_000913/1074341‑1074613

ttCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGcc                                                                                                                                       >  1:936679/1‑140 (MQ=255)

                              aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                                                                                                         >  1:282484/1‑140 (MQ=255)

                              aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                                                                                                         >  1:690005/1‑140 (MQ=255)

                              aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                                                                                                         >  1:417936/1‑140 (MQ=255)

                              aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                                                                                                         >  2:564279/1‑140 (MQ=255)

                              aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGc                                                                                                            >  2:499650/1‑137 (MQ=255)

                              aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGc                                                                                                            <  1:499650/137‑1 (MQ=255)

                                  cAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTg                                                                                                     >  1:438950/1‑140 (MQ=255)

                                  cAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTg                                                                                                     >  1:975689/1‑140 (MQ=255)

                                   agagTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTgg                                                                                                    >  2:44798/1‑140 (MQ=255)

                                         ggCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGa                                                                                              >  1:379011/1‑140 (MQ=255)

                                         ggCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGa                                                                                              >  1:352107/1‑140 (MQ=255)

                                          gCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggc                                                                                                   <  2:796846/134‑1 (MQ=255)

                                          gCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggc                                                                                                   >  1:796846/1‑134 (MQ=255)

                                            gggTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa                                                                                           <  1:550078/140‑1 (MQ=255)

                                            gggTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa                                                                                           <  2:417936/140‑1 (MQ=255)

                                            gggTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa                                                                                           <  2:282484/140‑1 (MQ=255)

                                               tgtTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGAt                                                                                             >  1:27377/1‑135 (MQ=255)

                                               tgtTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGAt                                                                                             <  2:27377/135‑1 (MQ=255)

                                                 tttCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAagag                                                                                      >  1:591630/1‑140 (MQ=255)

                                                     aaaaaCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACa                                                                                  <  2:980358/140‑1 (MQ=255)

                                                                   tgTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCtt                                                                    >  1:313624/1‑139 (MQ=255)

                                                                      aTTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGaa                                                                 <  1:820279/140‑1 (MQ=255)

                                                                               cGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCAcgc                                                        >  2:967723/1‑140 (MQ=255)

                                                                               cGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCAcgc                                                        >  1:586694/1‑140 (MQ=255)

                                                                                 tCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGa                                                      >  1:374213/1‑140 (MQ=255)

                                                                                 tCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGa                                                      >  2:435010/1‑140 (MQ=255)

                                                                                          gcgcTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCa                                             <  1:967723/140‑1 (MQ=255)

                                                                                               gTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCtt                                        <  1:435010/140‑1 (MQ=255)

                                                                                                    ttGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCgcgc                                   <  2:379011/140‑1 (MQ=255)

                                                                                                    ttGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCgcgc                                   <  2:352107/140‑1 (MQ=255)

                                                                                                    ttGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCgcgc                                   <  2:505367/140‑1 (MQ=255)

                                                                                                      gCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCt                                 <  2:656161/140‑1 (MQ=255)

                                                                                                         gTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGtt                              <  1:962455/140‑1 (MQ=255)

                                                                                                         gTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGtt                              <  2:690005/140‑1 (MQ=255)

                                                                                                          tgctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGtt                              >  1:639423/1‑139 (MQ=255)

                                                                                                          tgctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGtt                              <  2:639423/139‑1 (MQ=255)

                                                                                                          tgctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTc                             >  1:950657/1‑140 (MQ=255)

                                                                                                          tgctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTc                             >  1:768785/1‑140 (MQ=255)

                                                                                                           gctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCt                            <  1:564279/140‑1 (MQ=255)

                                                                                                                 gCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATgg                      >  2:145888/1‑140 (MQ=255)

                                                                                                                   aGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGgag                    <  2:591630/140‑1 (MQ=255)

                                                                                                                               tCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCGGgc        >  1:414537/1‑140 (MQ=255)

                                                                                                                                     tggcGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg  <  2:594326/140‑1 (MQ=255)

                                                                                                                                     tggcGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg  <  1:786718/140‑1 (MQ=255)

                                                                                                                                     tggcGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg  <  2:950657/140‑1 (MQ=255)

                                                                                                                                     tggcGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg  <  2:975689/140‑1 (MQ=255)

TTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCG  >  NC_000913/1074341‑1074613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A