Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,122,640	(T)_5→4	intergenic (‑33/+73)	pyrC ← / ← yceB	dihydroorotase/putative lipid‑binding lipoprotein YceB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,122,636	0	T	.	100.0%	156.0 / NA	36	intergenic (‑29/+77)	pyrC/yceB	dihydroorotase/putative lipid‑binding lipoprotein YceB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (25/11); total (25/11)

TCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCTTTCGCCA  >  NC_000913/1122504‑1122768

tCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGttttgccg                                                                                                                               <  1:942472/140‑5 (MQ=255)

  gCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGa                                                                                                                            >  1:123999/1‑140 (MQ=255)

                   cAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGa                                                                                                           >  1:72569/1‑140 (MQ=255)

                      tttttAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAAc                                                                                                        >  2:401824/1‑140 (MQ=255)

                      tttttAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAAc                                                                                                        >  2:1013581/1‑140 (MQ=255)

                       ttttAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATTCCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGGTAAGCGGAAACg                                                                                                       <  2:746102/140‑1 (MQ=255)

                         ttAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGtt                                                                                                     <  1:1013581/140‑1 (MQ=255)

                               tGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCtt                                                                                               >  1:643698/1‑140 (MQ=255)

                                gTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCttt                                                                                              >  1:1039784/1‑140 (MQ=255)

                                gTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCttt                                                                                              >  1:974524/1‑140 (MQ=255)

                                    ccATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCAc                                                                                          <  2:5000/140‑1 (MQ=255)

                                      aTCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGa                                                                                        <  2:72569/140‑1 (MQ=255)

                                                                cTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTc                                                              >  1:536141/1‑140 (MQ=255)

                                                                 tGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCg                                                             <  2:536141/140‑1 (MQ=255)

                                                                             cTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTa                                                       >  2:943151/1‑134 (MQ=255)

                                                                             cTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTa                                                       <  1:943151/134‑1 (MQ=255)

                                                                                       tGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATg                                       >  1:586049/1‑140 (MQ=255)

                                                                                        gggATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTa                                                >  1:717304/1‑130 (MQ=255)

                                                                                        gggATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTa                                                <  2:717304/130‑1 (MQ=255)

                                                                                        gggATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGa                                      >  2:1036812/1‑140 (MQ=255)

                                                                                        gggATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGa                                      >  2:639961/1‑140 (MQ=255)

                                                                                               tGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTc                               >  2:903107/1‑140 (MQ=255)

                                                                                               tGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTc                               >  2:372061/1‑140 (MQ=255)

                                                                                               tGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTc                               >  2:835479/1‑140 (MQ=255)

                                                                                               tGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTc                               >  1:715120/1‑140 (MQ=255)

                                                                                                        tAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATg                                       >  1:261369/1‑123 (MQ=255)

                                                                                                        tAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATg                                       <  2:261369/123‑1 (MQ=255)

                                                                                                        tAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGctt                      <  1:372061/140‑1 (MQ=255)

                                                                                                         aaTCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTCCGCCTGGcttc                     <  2:586049/140‑1 (MQ=255)

                                                                                                              ctATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGcttcacttc                >  2:147286/1‑140 (MQ=255)

                                                                                                              ctATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGcttcacttc                >  1:432456/1‑140 (MQ=255)

                                                                                                               tATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGc                             >  2:589042/1‑126 (MQ=255)

                                                                                                               tATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGc                             <  1:589042/126‑1 (MQ=255)

                                                                                                                   cTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGc           >  2:216613/1‑140 (MQ=255)

                                                                                                                      cGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCtt        >  1:435413/1‑140 (MQ=255)

                                                                                                                      cGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCtt        >  2:272862/1‑140 (MQ=255)

                                                                                                                            aaGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCTTTCGCCa  >  2:425550/1‑140 (MQ=255)

TCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCTTTCGCCA  >  NC_000913/1122504‑1122768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A