Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,127,049	A→G	T82A (ACG→GCG)	yceM →	putative oxidoreductase YceM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,127,049	0	A	G	100.0%	138.4 / NA	38	T82A (ACG→GCG)	yceM	putative oxidoreductase YceM
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/11); total (27/11)

GCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCA  >  NC_000913/1126913‑1127183

gCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGt                                                                                                                                     >  1:558181/1‑140 (MQ=255)

 ccTACGCGCGCGAAAGCCCTGCCAATTTGGGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGtt                                                                                                                                    >  2:289645/1‑140 (MQ=255)

  cTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTa                                                                                                                                   <  2:558181/140‑1 (MQ=255)

    aCGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCATTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACt                                                                                                                                 <  1:800224/140‑1 (MQ=255)

          gcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGc                                                                                                                           <  2:220360/140‑1 (MQ=255)

                     gCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATg                                                                                                                <  2:486283/140‑1 (MQ=255)

                         aTTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCtg                                                                                                            <  1:289645/140‑1 (MQ=255)

                                       gcgcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGc                                                                                              >  1:1016743/1‑140 (MQ=255)

                                            ttCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCa                                                                                         >  1:23167/1‑140 (MQ=255)

                                                    gCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCt                                                                                 >  2:477927/1‑140 (MQ=255)

                                                        aTTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgcgc                                                                             >  1:688147/1‑140 (MQ=255)

                                                         ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                                                            >  1:329648/1‑140 (MQ=255)

                                                         ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                                                            >  1:391808/1‑140 (MQ=255)

                                                         ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                                                            >  2:610909/1‑140 (MQ=255)

                                                         ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                                                            >  1:490741/1‑140 (MQ=255)

                                                          tCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGa                                                                           <  1:388155/140‑1 (MQ=255)

                                                                   aGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACg                                                                  >  1:1072131/1‑140 (MQ=255)

                                                                     ccTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGc                                                                >  1:1142119/1‑140 (MQ=255)

                                                                            gccAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGa                                                         <  1:477927/140‑1 (MQ=255)

                                                                                           gTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaa                                          >  2:1036837/1‑140 (MQ=255)

                                                                                           gTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaa                                          <  2:490741/140‑1 (MQ=255)

                                                                                                 gtgCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACt                                    >  2:346202/1‑140 (MQ=255)

                                                                                                        ccAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTg                             <  1:1104673/140‑1 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGTAAAAACTGACGTTGATGGTCGGt                    >  2:1006442/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:565618/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:715295/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:463344/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:769045/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:102474/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:143160/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:858797/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:480242/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:460649/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:199189/1‑140 (MQ=255)

                                                                                                                 ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGAGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:68919/1‑140 (MQ=255)

                                                                                                                              cGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGtt       >  1:109487/1‑140 (MQ=255)

                                                                                                                                 ggTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCg    <  2:1142119/140‑1 (MQ=255)

                                                                                                                                   tCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGca  <  1:610909/140‑1 (MQ=255)

GCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCA  >  NC_000913/1126913‑1127183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A