Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F28 I1 R1
|
162 |
93.0 |
3098984 |
99.0% |
3067994 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,129,332 |
A→G |
V498V (GTA→GTG) |
murJ → |
putative lipid II flippase MurJ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,129,332 | 0 | A | G | 96.8%
| 101.8
/ ‑4.4
| 31 | V498V (GTA→GTG) | murJ | putative lipid II flippase MurJ |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (1/29); total (1/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 |
TGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCC > NC_000913/1129196‑1129468
|
tGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTg < 1:107640/140‑1 (MQ=255)
tGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTc < 1:255917/140‑1 (MQ=255)
cGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTa < 2:684321/140‑1 (MQ=255)
ttACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCccgccg < 2:805854/140‑1 (MQ=255)
aCATATCGTGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa < 1:449124/140‑1 (MQ=255)
aCATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa < 2:110899/140‑1 (MQ=255)
aCATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa < 1:243606/140‑1 (MQ=255)
aCATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa < 2:753103/140‑1 (MQ=255)
aCATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa < 1:566045/140‑1 (MQ=255)
aCATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa < 2:796722/140‑1 (MQ=255)
cATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtg < 1:823380/140‑1 (MQ=255)
tGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgca < 1:205537/140‑1 (MQ=255)
ggCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCgg < 2:100899/140‑1 (MQ=255)
tAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATccggcctg < 1:391777/140‑8 (MQ=255)
ggCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGaa < 2:1258066/140‑1 (MQ=255)
ggCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGaa < 1:639553/140‑1 (MQ=255)
cGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAt < 1:187732/140‑1 (MQ=255)
ggCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccg < 1:871484/140‑1 (MQ=255)
ggATTGCCGCGTACTTCGCTGCCCTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCcgcg < 1:1145865/140‑1 (MQ=255)
gCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg < 1:607207/140‑1 (MQ=255)
gCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg < 1:482389/140‑1 (MQ=255)
gCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg < 1:789169/140‑1 (MQ=255)
gCCGCGTACTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg < 2:1251810/140‑1 (MQ=255)
accGCGTCCTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGGCGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg < 1:1285141/139‑1 (MQ=255)
cTTCGCTGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAg < 1:1470709/140‑1 (MQ=255)
tGCACTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGa < 1:973318/140‑1 (MQ=255)
cTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt < 1:1045822/140‑1 (MQ=255)
cTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt < 1:615906/140‑1 (MQ=255)
cTGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt < 1:307727/140‑1 (MQ=255)
tGGCGGTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGGCGGGGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGGTGTTGAGGTCTGACCGtt > 1:806143/1‑140 (MQ=255)
ggTGCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc < 1:69594/140‑1 (MQ=255)
|
TGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCC > NC_000913/1129196‑1129468
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
