Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,482,477	A→G	G128G (GGT→GGC)	azoR ←	FMN dependent NADH:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,482,477	0	A	G	100.0%	175.7 / NA	47	G128G (GGT→GGC)	azoR	FMN dependent NADH:quinone oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/29); total (18/29)

CAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAATAACGATAACGTCGTGGGCTTTCAGCT  >  NC_000913/1482347‑1482613

cAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGa                                                                                                                                 <  1:176993/140‑1 (MQ=255)

  aaTTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGAcc                                                                                                                               <  1:524513/140‑1 (MQ=255)

   aTTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCg                                                                                                                              >  1:212209/1‑140 (MQ=255)

       cacaTCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTtct                                                                                                                          <  2:251282/140‑1 (MQ=255)

       cacaTCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTtct                                                                                                                          <  1:676759/140‑1 (MQ=255)

                  tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCgg                                                                                                               <  2:687890/140‑1 (MQ=255)

                  tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCgg                                                                                                               <  2:212209/140‑1 (MQ=255)

                  tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCgg                                                                                                               <  1:25599/140‑1 (MQ=255)

                         aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTaa                                                                                                        >  1:599994/1‑140 (MQ=255)

                            gCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGc                                                                                                     <  2:1045510/140‑1 (MQ=255)

                               gAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTg                                                                                                  <  1:34923/140‑1 (MQ=255)

                                 ggAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTgcg                                                                                                <  2:571474/140‑1 (MQ=255)

                                              aTACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGt                                                                                   <  1:438824/140‑1 (MQ=255)

                                              aTACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGt                                                                                   >  2:452500/1‑140 (MQ=255)

                                              aTACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCAGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGt                                                                                   <  2:925564/140‑1 (MQ=255)

                                                    cGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAAt                                                                             >  1:406146/1‑140 (MQ=255)

                                                        aCCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGtt                                                                         >  2:733728/1‑140 (MQ=255)

                                                             gTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCa                                                                    >  1:590060/1‑140 (MQ=255)

                                                                      tCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCg                                                           <  1:34855/140‑1 (MQ=255)

                                                                       ccATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCga                                                          >  2:584437/1‑140 (MQ=255)

                                                                       ccATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCga                                                          >  2:329461/1‑140 (MQ=255)

                                                                       ccATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCga                                                          <  1:935875/140‑1 (MQ=255)

                                                                         aTCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCgaga                                                        <  2:406146/140‑1 (MQ=255)

                                                                            tttGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGt                                                     <  2:590060/140‑1 (MQ=255)

                                                                             ttGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAATTGAGTCGAGATGtt                                                    <  1:814910/140‑1 (MQ=255)

                                                                             ttGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGtt                                                    <  2:464619/140‑1 (MQ=255)

                                                                              tgtgGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  1:187878/140‑1 (MQ=255)

                                                                              tgtgGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  2:452183/140‑1 (MQ=255)

                                                                              tgtgGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  2:446427/140‑1 (MQ=255)

                                                                               gtgGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGa                                                  <  2:821396/140‑1 (MQ=255)

                                                                                    tCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATg                                                      <  1:950972/131‑1 (MQ=255)

                                                                                    tCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGGCGAGATg                                                      >  2:950972/1‑131 (MQ=255)

                                                                                       tgccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  1:79364/130‑1 (MQ=255)

                                                                                       tgccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   >  2:79364/2‑131 (MQ=255)

                                                                                       cgccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  2:608927/131‑1 (MQ=255)

                                                                                       cgccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   >  1:608927/1‑131 (MQ=255)

                                                                                         ccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACAt                                        <  2:599994/140‑1 (MQ=255)

                                                                                            cgGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCgg                                     <  1:819433/140‑1 (MQ=255)

                                                                                            cgGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCgg                                     <  1:551275/140‑1 (MQ=255)

                                                                                              gCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTg                                   >  2:904382/1‑140 (MQ=255)

                                                                                                            tGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgata                     >  1:267983/1‑140 (MQ=255)

                                                                                                            tGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgat                      >  2:1016965/1‑139 (MQ=255)

                                                                                                            tGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgat                      <  1:1016965/139‑1 (MQ=255)

                                                                                                             ggCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgataa                    >  2:302096/1‑140 (MQ=255)

                                                                                                             ggCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgataa                    >  2:172774/1‑140 (MQ=255)

                                                                                                             ggCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgataa                    >  1:789338/1‑140 (MQ=255)

                                                                                                                            tACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAATAACGATAACGTCGTGGGCTTTCa     >  2:378123/1‑140 (MQ=255)

                                                                                                                               cAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAATAACGATAACGTCGTGGGCTTTCAGCt  <  2:371122/140‑1 (MQ=255)

CAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAATAACGATAACGTCGTGGGCTTTCAGCT  >  NC_000913/1482347‑1482613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A