Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,485,103	(G)_5→4	coding (2043/3903 nt)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,485,099	0	G	.	100.0%	246.6 / NA	57	coding (2039/3903 nt)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (31/26); total (31/26)

ATGAAAGATGCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGT  >  NC_000913/1484966‑1485231

aTGAAAGATGCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTggggcgc                                                                                                                                <  1:1034638/140‑4 (MQ=255)

    aaGATGCCGATAAACAAGAATATATCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATtg                                                                                                                           >  1:538185/1‑140 (MQ=255)

    aaGATGCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATtg                                                                                                                           <  1:582002/140‑1 (MQ=255)

         gCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATtgct                                                                                                                         <  1:541321/137‑1 (MQ=255)

         gCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATtgct                                                                                                                         >  2:541321/1‑137 (MQ=255)

                 aCAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGa                                                                                                              >  1:417459/1‑140 (MQ=255)

                  cAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTAT‑GGGGCGCATTGCTGCGCGTATCGAc                                                                                                             <  2:322699/140‑1 (MQ=255)

                          taCCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATg                                                                                                     >  2:347258/1‑140 (MQ=255)

                           aCCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATgg                                                                                                    >  2:543044/1‑140 (MQ=255)

                            ccGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATggg                                                                                                   <  2:277888/140‑1 (MQ=255)

                               gcgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGaa                                                                                                       <  1:418593/133‑1 (MQ=255)

                               gcgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGaa                                                                                                       >  2:418593/1‑133 (MQ=255)

                                cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTAt                                                                                                                 >  2:103739/1‑122 (MQ=255)

                                cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTAt                                                                                                                 <  1:103739/122‑1 (MQ=255)

                                cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGaa                                                                                                       <  2:626813/132‑1 (MQ=255)

                                cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGaa                                                                                                       >  1:626813/1‑132 (MQ=255)

                                cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGa                                                                                               >  1:500268/1‑140 (MQ=255)

                                  cACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGc                                                                                             >  2:292427/1‑140 (MQ=255)

                                     gTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAg                                                                                          <  2:500268/140‑1 (MQ=255)

                                          gcgcgTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     <  1:1031915/140‑1 (MQ=255)

                                               tttCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCa                                                                                <  2:338124/140‑1 (MQ=255)

                                               tttCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCa                                                                                >  2:1075396/1‑140 (MQ=255)

                                               tttCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCa                                                                                <  1:825877/140‑1 (MQ=255)

                                                      aTCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGAtt                                                                         >  2:730673/1‑140 (MQ=255)

                                                        cTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTaa                                                                       >  1:322905/1‑140 (MQ=255)

                                                        cTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTaa                                                                       >  2:302070/1‑140 (MQ=255)

                                                         ttCCCCGGTTTTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTaaa                                                                      <  1:456024/140‑1 (MQ=255)

                                                         ttCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTaaa                                                                      <  1:730673/140‑1 (MQ=255)

                                                          tCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     <  1:1061167/124‑1 (MQ=255)

                                                          tCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     >  2:1061167/1‑124 (MQ=255)

                                                          tCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAAc                                                                     >  1:548426/1‑140 (MQ=255)

                                                           gcgcGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACg                                                                    <  1:567134/137‑1 (MQ=255)

                                                            cccGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     >  2:1186429/1‑122 (MQ=255)

                                                            cccGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     <  1:1186429/122‑1 (MQ=255)

                                                            cccGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGc                                                                   >  1:1113738/1‑140 (MQ=255)

                                                               ggTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCAcc                                                                >  2:865968/1‑140 (MQ=255)

                                                                  tCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTAc                                                             >  2:1041836/1‑140 (MQ=255)

                                                                   cTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACa                                                            >  1:412516/1‑140 (MQ=255)

                                                                        ttATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGaa                                                       <  1:1075396/140‑1 (MQ=255)

                                                                         tATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAAc                                                      <  1:390848/140‑1 (MQ=255)

                                                                           ttCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCg                                                    >  1:882724/1‑140 (MQ=255)

                                                                                    ccGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGaa                                           <  1:347258/140‑1 (MQ=255)

                                                                                            aTGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCa                                   <  1:543044/140‑1 (MQ=255)

                                                                                               ggTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAggg                                >  1:1190599/1‑140 (MQ=255)

                                                                                                 tAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGgcg                              <  1:645245/140‑1 (MQ=255)

                                                                                                  aaTGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGgc                               >  2:1195973/1‑138 (MQ=255)

                                                                                                  aaTGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGgc                               <  1:1195973/138‑1 (MQ=255)

                                                                                                      gtggCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTg                         >  2:463086/1‑140 (MQ=255)

                                                                                                         gCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATg                      >  1:1191590/1‑140 (MQ=255)

                                                                                                          cGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATgg                     >  2:1188791/1‑140 (MQ=255)

                                                                                                                 ggTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGa              <  2:412516/140‑1 (MQ=255)

                                                                                                                 ggTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGa              <  1:1188791/140‑1 (MQ=255)

                                                                                                                   tAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGaaa            >  1:163086/1‑140 (MQ=255)

                                                                                                                        aCCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTc       >  2:351927/1‑140 (MQ=255)

                                                                                                                         ccAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCa      <  1:865968/140‑1 (MQ=255)

                                                                                                                         ccAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCa      <  1:123818/140‑1 (MQ=255)

                                                                                                                            gccgccTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTg   >  1:702879/1‑140 (MQ=255)

                                                                                                                             ccgccTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGt  >  1:970754/1‑140 (MQ=255)

ATGAAAGATGCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGT  >  NC_000913/1484966‑1485231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A