Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,476	T→C	V307A (GTA→GCA)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,476	0	T	C	100.0%	131.3 / NA	37	V307A (GTA→GCA)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/16); total (21/16)

GCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACC  >  NC_000913/1680340‑1680609

gCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCAcc                                                                                                                                    >  2:758250/1‑140 (MQ=255)

gCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCAcc                                                                                                                                    >  1:236181/1‑140 (MQ=255)

        tCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTgg                                                                                                                            <  2:960455/140‑1 (MQ=255)

            aaCCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGgccgc                                                                                                                        <  2:193275/140‑1 (MQ=255)

             aCCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGgccgcc                                                                                                                       <  2:236181/140‑1 (MQ=255)

              cccGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCa                                                                                                                      <  2:328874/140‑1 (MQ=255)

                  tCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCa                                                                                                                  >  2:351419/1‑140 (MQ=255)

                   cAATGGCTGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCat                                                                                                                     >  2:475443/1‑135 (MQ=255)

                   cAATGGCTGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCat                                                                                                                     <  1:475443/136‑2 (MQ=255)

                        gCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGc                                                                                                            >  1:101647/1‑140 (MQ=255)

                            gTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAATCACCGTTCCTGGCCGCCATTCATAAAGCATTc                                                                                                        >  1:95530/1‑140 (MQ=255)

                            gTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCatt                                                                                                                    >  1:151736/1‑126 (MQ=255)

                            gTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCatt                                                                                                                    <  2:151736/128‑3 (MQ=255)

                                gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTccc                                                                                                      >  2:821929/1‑138 (MQ=255)

                                gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTccc                                                                                                      <  1:821929/138‑1 (MQ=255)

                                  tggtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCgcgc                                                                                                  >  1:629224/1‑140 (MQ=255)

                                    gtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCAt                                                                                                <  1:182509/140‑1 (MQ=255)

                                           tgatgGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTgcg                                                                                         >  2:288133/1‑140 (MQ=255)

                                             atgGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTgcgcg                                                                                       >  2:372211/1‑140 (MQ=255)

                                                       ggggCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGca                                                                             >  2:823861/1‑140 (MQ=255)

                                                              aaTCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTg                                                                      >  1:255568/1‑140 (MQ=255)

                                                                    catcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCAt                                                                <  1:1006088/140‑1 (MQ=255)

                                                                    catcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCAt                                                                <  2:629224/140‑1 (MQ=255)

                                                                        gCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGc                                                            <  2:380872/140‑1 (MQ=255)

                                                                            ccGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCa                                                        >  1:701877/1‑140 (MQ=255)

                                                                             cGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCAc                                                       <  1:288133/140‑1 (MQ=255)

                                                                                          tCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCaa                                          >  1:458295/1‑140 (MQ=255)

                                                                                               ttGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCt                                     <  1:758250/140‑1 (MQ=255)

                                                                                                              aaGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTc                      <  2:201255/140‑1 (MQ=255)

                                                                                                                    gACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGTGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTAc                >  1:758839/1‑140 (MQ=255)

                                                                                                                      ccATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTc              >  2:905206/1‑140 (MQ=255)

                                                                                                                         tcatGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATc           <  2:255568/140‑1 (MQ=255)

                                                                                                                          catGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCt          >  1:925710/1‑140 (MQ=255)

                                                                                                                              gcagcaGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCt      >  1:862175/1‑140 (MQ=255)

                                                                                                                                 gcagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGa    >  2:303338/1‑139 (MQ=255)

                                                                                                                                 gcagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGa    <  1:303338/139‑1 (MQ=255)

                                                                                                                                  cagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGAcc  >  2:727418/1‑140 (MQ=255)

GCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACC  >  NC_000913/1680340‑1680609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A