Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,494	C→T	T313I (ACT→ATT)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,494	0	C	T	100.0%	110.9 / NA	32	T313I (ACT→ATT)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/13); total (19/13)

TCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACA  >  NC_000913/1680358‑1680625

tCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCa                                                                                                                                  >  2:351419/1‑140 (MQ=255)

      gCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGc                                                                                                                            >  1:101647/1‑140 (MQ=255)

          gTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAATCACCGTTCCTGGCCGCCATTCATAAAGCATTc                                                                                                                        >  1:95530/1‑140 (MQ=255)

              gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTccc                                                                                                                      >  2:821929/1‑138 (MQ=255)

              gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTccc                                                                                                                      <  1:821929/138‑1 (MQ=255)

                tggtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCgcgc                                                                                                                  >  1:629224/1‑140 (MQ=255)

                  gtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCAt                                                                                                                <  1:182509/140‑1 (MQ=255)

                         tgatgGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTgcg                                                                                                         >  2:288133/1‑140 (MQ=255)

                           atgGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTgcgcg                                                                                                       >  2:372211/1‑140 (MQ=255)

                                     ggggCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGca                                                                                             >  2:823861/1‑140 (MQ=255)

                                            aaTCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTg                                                                                      >  1:255568/1‑140 (MQ=255)

                                                  catcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCAt                                                                                <  2:629224/140‑1 (MQ=255)

                                                  catcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCAt                                                                                <  1:1006088/140‑1 (MQ=255)

                                                      gCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGc                                                                            <  2:380872/140‑1 (MQ=255)

                                                          ccGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCa                                                                        >  1:701877/1‑140 (MQ=255)

                                                           cGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCAc                                                                       <  1:288133/140‑1 (MQ=255)

                                                                        tCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCaa                                                          >  1:458295/1‑140 (MQ=255)

                                                                             ttGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCt                                                     <  1:758250/140‑1 (MQ=255)

                                                                                            aaGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTc                                      <  2:201255/140‑1 (MQ=255)

                                                                                                  gACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGTGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTAc                                >  1:758839/1‑140 (MQ=255)

                                                                                                    ccATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTc                              >  2:905206/1‑140 (MQ=255)

                                                                                                       tcatGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATc                           <  2:255568/140‑1 (MQ=255)

                                                                                                        catGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCt                          >  1:925710/1‑140 (MQ=255)

                                                                                                            gcagcaGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCt                      >  1:862175/1‑140 (MQ=255)

                                                                                                               gcagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGa                    >  2:303338/1‑139 (MQ=255)

                                                                                                               gcagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGa                    <  1:303338/139‑1 (MQ=255)

                                                                                                                cagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGAcc                  >  2:727418/1‑140 (MQ=255)

                                                                                                                      ccccGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTcc            <  2:101647/138‑1 (MQ=255)

                                                                                                                          gTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGAtt        >  2:685160/1‑140 (MQ=255)

                                                                                                                             ccTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTaca     >  2:377081/1‑140 (MQ=255)

                                                                                                                                ggCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca  <  1:82319/140‑1 (MQ=255)

                                                                                                                                ggCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca  <  1:685160/140‑1 (MQ=255)

TCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACA  >  NC_000913/1680358‑1680625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A