Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F28 I1 R1
|
138 |
62.6 |
2119710 |
99.4% |
2106991 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,775,461 |
G→A |
intergenic (+17/‑126) |
aroD → / → ydiF |
3‑dehydroquinate dehydratase/putative acetate‑CoA transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,775,461 | 0 | G | A | 100.0%
| 58.5
/ NA
| 19 | intergenic (+17/‑126) | aroD/ydiF | 3‑dehydroquinate dehydratase/putative acetate‑CoA transferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/8); total (11/8) |
TGGCTGGTGAAGTATTTGGCTCGGCGGCAACTTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTG > NC_000913/1775326‑1775596
|
tggctggTGAAGTATTTGGCTCGGCGGCAACTTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCAGGaa < 1:415993/140‑1 (MQ=255)
gctggTGAAGTATTTGGCTCGGCGGCAACTTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCAGGAaca < 2:148163/140‑1 (MQ=255)
gTATTTGGCCCGGCGGCAACTTTTGGTGCGGTAAAAAAAGCGTCTGCCCCGGGGCAAATCTCGGAAAATGATTTGCGCCAGGTATAAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCAGGAACACCCTCCccg < 2:67918/140‑1 (MQ=255)
gCTCGGCGGCAACTTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACCCCAGGCATAAGCAATAATATTTCGGCAGGAACCCCCTCCCCGCCGAACt < 2:83444/140‑1 (MQ=255)
gcggcAACTTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCAGGAACCCCCTCCCCGCCGAACTaaaaa < 2:825569/140‑1 (MQ=255)
aaCTTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAatata < 1:74427/140‑1 (MQ=255)
tttttGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGAAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGAAATAATATTTCGGCAGGAACCCCCTCCCCGCCGAACTAAAAAATATAtt < 1:1024627/139‑1 (MQ=255)
aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCtgtg > 1:883558/1‑140 (MQ=255)
cACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCAGGAACCCCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCg < 1:868267/140‑1 (MQ=255)
tttACACCAGGCATAAGCAATAATATTTCGGCAGGAACCCCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt < 1:30799/140‑1 (MQ=255)
tttACACCAGGCATAAGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt > 1:136458/1‑140 (MQ=255)
tttACACCAGGCATAAGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt > 2:405571/1‑140 (MQ=255)
tttACACCAGGCATAAGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt > 2:30799/1‑140 (MQ=255)
acCAGGCATAAGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGaa > 2:1050066/1‑140 (MQ=255)
ggCATAAGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAg > 1:975725/1‑140 (MQ=255)
ggCATAAGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAg > 1:115542/1‑140 (MQ=255)
aaGCAATAATATTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAtt > 2:800362/1‑140 (MQ=255)
taataTTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAAt > 2:166710/1‑140 (MQ=255)
taataTTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAAt > 1:999498/1‑140 (MQ=255)
taataTTTCGGCAGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAAt > 1:55506/1‑140 (MQ=255)
cggcagGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTg > 1:396557/1‑140 (MQ=255)
cggcagGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTg > 1:305662/1‑140 (MQ=255)
|
TGGCTGGTGAAGTATTTGGCTCGGCGGCAACTTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTG > NC_000913/1775326‑1775596
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
