Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,573,760	G→A	L182L (CTC→CTT)	eutT ←	putative ethanolamine utilization cobalamin adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,573,760	0	G	A	100.0%	105.4 / NA	30	L182L (CTC→CTT)	eutT	putative ethanolamine utilization cobalamin adenosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/19); total (11/19)

GCGTAATACTTCAAAGCTGCGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGC  >  NC_000913/2573625‑2573891

gCGTAATACTTCAAAGCTGCGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGAt                                                                                                                                 <  2:310856/140‑1 (MQ=255)

          tCAAAGCTGCGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCgg                                                                                                                       <  2:596/140‑1 (MQ=255)

                   cGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATgaga                                                                                                              >  1:723084/1‑140 (MQ=255)

                       tGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGACACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGc                                                                                                          <  1:204766/140‑1 (MQ=255)

                        gATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGcc                                                                                                         <  2:956474/140‑1 (MQ=255)

                           aaaGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGAt                                                                                                      <  2:327328/140‑1 (MQ=255)

                                ccTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGa                                                                                                 >  2:602148/1‑140 (MQ=255)

                                 cTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATgag                                                                                                               <  1:293466/125‑1 (MQ=255)

                                 cTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATgag                                                                                                               >  2:293466/1‑125 (MQ=255)

                                    cgccgcTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCtt                                                                                             >  1:665231/1‑140 (MQ=255)

                                               tttcggtttcCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGc                                                                                  <  1:548713/140‑1 (MQ=255)

                                                    gtttcCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGAc                                                                             >  2:864024/1‑140 (MQ=255)

                                                         ccGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCg                                                                        >  1:647986/1‑140 (MQ=255)

                                                              cTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTgg                                                                   <  2:248431/140‑1 (MQ=255)

                                                                 tGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAg                                                                <  2:647986/140‑1 (MQ=255)

                                                                     gCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCa                                                            <  1:481311/140‑1 (MQ=255)

                                                                        tAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCGGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCg                                                         <  1:585333/140‑1 (MQ=255)

                                                                                tGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTccccc                                                 >  1:1039582/1‑140 (MQ=255)

                                                                                        gcagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATc                                         >  1:385217/1‑140 (MQ=255)

                                                                                        gcagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATc                                         >  2:80001/1‑140 (MQ=255)

                                                                                          agcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCgg                                       <  2:1039582/140‑1 (MQ=255)

                                                                                          agcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCgg                                       <  1:145059/140‑1 (MQ=255)

                                                                                           gcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGgc                                      <  2:385217/140‑1 (MQ=255)

                                                                                           gcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGgc                                      <  1:836232/140‑1 (MQ=255)

                                                                                              tCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGgcgcg                                   <  1:274377/140‑1 (MQ=255)

                                                                                               cgcgCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGgcgcgc                                  <  1:864024/140‑1 (MQ=255)

                                                                                                                         aGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGTGCGCATAATGTTGCCAAGACGCGAACGGat        >  2:286267/1‑140 (MQ=255)

                                                                                                                              atCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCg   <  2:723084/140‑1 (MQ=255)

                                                                                                                               tCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgc  >  2:141316/1‑140 (MQ=255)

                                                                                                                                  tGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACg           >  1:845782/1‑128 (MQ=255)

                                                                                                                                  tGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACg           <  2:845782/128‑1 (MQ=255)

GCGTAATACTTCAAAGCTGCGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGC  >  NC_000913/2573625‑2573891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A