Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,836,245	A→G	V394A (GTG→GCG)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,836,245	0	A	G	100.0%	163.7 / NA	45	V394A (GTG→GCG)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/24); total (21/24)

ATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATG  >  NC_000913/2836113‑2836375

aTCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCgg                                                                                                                             <  2:758234/140‑1 (MQ=255)

aTCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCgg                                                                                                                             <  1:321767/140‑1 (MQ=255)

    ggCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACa                                                                                                                         >  2:177187/1‑140 (MQ=255)

               tCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGcc                                                                                                              <  1:172531/140‑1 (MQ=255)

               tCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGcc                                                                                                              >  2:786204/1‑140 (MQ=255)

                 gcgcCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCg                                                                                                            <  2:612890/140‑1 (MQ=255)

                 gcgcCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCg                                                                                                            <  1:1012020/140‑1 (MQ=255)

                               ggATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAgc                                                                                              >  2:894745/1‑140 (MQ=255)

                                  tGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCa                                                                                           >  2:503513/1‑140 (MQ=255)

                                  tGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCa                                                                                           >  1:975750/1‑140 (MQ=255)

                                    ccATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATc                                                                                         <  1:138119/140‑1 (MQ=255)

                                    ccATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATc                                                                                         <  1:272200/140‑1 (MQ=255)

                                    ccATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATc                                                                                         <  2:1037420/140‑1 (MQ=255)

                                      aTCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCg                                                                                       <  2:975750/140‑1 (MQ=255)

                                          tCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGcac                                                                                   >  1:405741/1‑140 (MQ=255)

                                          tCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGcaa                                                                                   >  1:688646/1‑139 (MQ=255)

                                              ttAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATAc                                                                               >  1:690645/1‑140 (MQ=255)

                                                      cccAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGa                                                                       >  2:1001106/1‑140 (MQ=255)

                                                        cAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCg                                                                                       >  2:493810/1‑122 (MQ=255)

                                                        cAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCg                                                                                       <  1:493810/122‑1 (MQ=255)

                                                             gCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGgcgc                                                                >  1:139746/1‑140 (MQ=255)

                                                                         ccGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTcgccg                                                    <  1:786204/140‑1 (MQ=255)

                                                                           gCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTcgccg                                                    <  2:833912/138‑1 (MQ=255)

                                                                           gCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTcgccg                                                    >  1:833912/1‑138 (MQ=255)

                                                                           gCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCt                                                  >  1:486092/1‑140 (MQ=255)

                                                                             ttGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCttt                                                >  2:93285/1‑140 (MQ=255)

                                                                                 tCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTcgcg                                            <  2:486092/140‑1 (MQ=255)

                                                                                 tCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTcgcg                                            <  1:58979/140‑1 (MQ=255)

                                                                                 tCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTcgcg                                            <  1:177187/140‑1 (MQ=255)

                                                                                   aCCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGca                                          <  1:673533/140‑1 (MQ=255)

                                                                                     cgcgCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGcacc                                        >  1:60638/1‑140 (MQ=255)

                                                                                      gcgcACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGcacca                                       >  2:134444/1‑140 (MQ=255)

                                                                                       cgcACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGcaccac                                      >  2:823150/1‑140 (MQ=255)

                                                                                          aCCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATTCCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGa                                   <  1:93285/140‑1 (MQ=255)

                                                                                                   aaGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATcatccat                          >  2:1038210/1‑140 (MQ=255)

                                                                                                   aaGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATcatccat                          >  1:86585/1‑140 (MQ=255)

                                                                                                      gTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCGCCGAATCATCCATCCg                       <  1:134444/140‑1 (MQ=255)

                                                                                                           tttCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCa                  <  1:503513/140‑1 (MQ=255)

                                                                                                             tCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACg                <  2:60638/140‑1 (MQ=255)

                                                                                                                 aTCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCa                  <  2:746297/134‑1 (MQ=255)

                                                                                                                 aTCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCa                  >  1:746297/1‑134 (MQ=255)

                                                                                                                   ccGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg          <  2:690645/140‑1 (MQ=255)

                                                                                                                   ccGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg          <  1:1038210/140‑1 (MQ=255)

                                                                                                                      cgcCGAGACACAGCGCAGGCGCAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCgg       >  2:752213/1‑140 (MQ=255)

                                                                                                                          gagaCACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTAt   <  1:894745/140‑1 (MQ=255)

                                                                                                                           agaCACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATg  >  2:220903/1‑140 (MQ=255)

ATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATG  >  NC_000913/2836113‑2836375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A