Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 162 93.0 3098984 99.0% 3067994 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,353,137 A→G C164C (TGT→TGC)  sapD ← putrescine ABC exporter ATP binding protein SapD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,353,1370AG98.9% 333.6 / ‑4.7 90C164C (TGT→TGC) sapDputrescine ABC exporter ATP binding protein SapD
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (42/47);  total (43/47)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTC  >  NC_000913/1353000‑1353272
                                                                                                                                         |                                                                                                                                       
gTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCa                                                                                                                                       >  1:719382/1‑140 (MQ=255)
gTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCa                                                                                                                                       >  1:1180956/1‑140 (MQ=255)
  aTTGCTGTTTTGGTTGAGACGCGTGAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCAtt                                                                                                                                     <  2:773752/140‑1 (MQ=255)
  aTTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCCCTTTCTGGCAtt                                                                                                                                     <  2:880729/140‑1 (MQ=255)
   ttGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTc                                                                                                                                    >  2:880413/1‑140 (MQ=255)
    tGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCa                                                                                                                                   >  1:1209475/1‑140 (MQ=255)
     gCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCAc                                                                                                                                  >  2:526537/1‑140 (MQ=255)
       tGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCt                                                                                                                                <  2:1172301/140‑1 (MQ=255)
       tGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCt                                                                                                                                <  2:365126/140‑1 (MQ=255)
        gTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCtt                                                                                                                               <  2:88569/140‑1 (MQ=255)
         ttttGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTc                                                                                                                              >  2:1327544/1‑140 (MQ=255)
           ttGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCgg                                                                                                                            >  2:808732/1‑140 (MQ=255)
           ttGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGACGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACGTTCTGGCATTCACCTTCgg                                                                                                                            >  2:1327370/1‑140 (MQ=255)
            tGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGt                                                                                                                           <  1:808732/140‑1 (MQ=255)
             ggTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTc                                                                                                                          <  1:730251/140‑1 (MQ=255)
                  agtcgcgTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTc                                                                                                                     <  1:1546172/137‑1 (MQ=255)
                  agaCGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCTTTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTc                                                                                                                     <  1:172458/140‑1 (MQ=255)
                  agaCGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTc                                                                                                                     <  2:769740/140‑1 (MQ=255)
                    aCGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCAt                                                                                                                   <  2:56279/140‑1 (MQ=255)
                        gTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAggg                                                                                                               >  2:157040/1‑140 (MQ=255)
                        gTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAggg                                                                                                               >  2:115655/1‑140 (MQ=255)
                         tCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGa                                                                                                              >  2:1532243/1‑140 (MQ=255)
                                 cGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTgcg                                                                                                      <  1:526537/140‑1 (MQ=255)
                                  gAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTgcgc                                                                                                     <  1:361756/140‑1 (MQ=255)
                                   aaaGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGcgca                                                                                                    <  1:345728/140‑1 (MQ=255)
                                      gATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGcgcatcg                                                                                                 <  2:719382/140‑1 (MQ=255)
                                      gATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGcgcatcg                                                                                                 <  2:1382682/140‑1 (MQ=255)
                                      gATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGcgcatcg                                                                                                 <  2:1427938/140‑1 (MQ=255)
                                       aTTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGcgcatcgc                                                                                                <  1:601882/140‑1 (MQ=255)
                                        tttGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGcgcatcgca                                                                                               <  2:837161/140‑1 (MQ=255)
                                        tttGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGcgcatcgca                                                                                               <  1:1352138/140‑1 (MQ=255)
                                          tGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCgcatcgcatc                                                                                             >  2:1299908/1‑140 (MQ=255)
                                                tGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTtgtg                                                                                       <  1:825769/140‑1 (MQ=255)
                                                 gggTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGa                                                                                      >  1:251854/1‑140 (MQ=255)
                                                           cTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATcc                                                                            <  2:1516567/140‑1 (MQ=255)
                                                            tCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATccc                                                                           >  1:609763/1‑140 (MQ=255)
                                                             ccATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATcccc                                                                          >  1:528295/1‑140 (MQ=255)
                                                             ccATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATcccc                                                                          >  1:1148034/1‑140 (MQ=255)
                                                              cATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCa                                                                         <  2:1301001/140‑1 (MQ=255)
                                                              cATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCa                                                                         <  2:1340513/140‑1 (MQ=255)
                                                              cATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCa                                                                         <  1:880413/140‑1 (MQ=255)
                                                              cATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCa                                                                         >  2:1028754/1‑140 (MQ=255)
                                                                ttGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCAcg                                                                       <  1:1068720/140‑1 (MQ=255)
                                                                ttGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCAcg                                                                       <  2:1209475/140‑1 (MQ=255)
                                                                ttGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCAcg                                                                       <  1:1327544/140‑1 (MQ=255)
                                                                ttGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCAcg                                                                       <  2:1329811/140‑1 (MQ=255)
                                                                   aGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCgg                                                                    <  1:115655/140‑1 (MQ=255)
                                                                   aGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCgg                                                                    <  1:310332/140‑1 (MQ=255)
                                                                        gTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTgcag                                                               >  1:1088207/1‑140 (MQ=255)
                                                                        gTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTgcag                                                               <  2:1110351/140‑1 (MQ=255)
                                                                          cGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAGCTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTgcagca                                                             >  2:607926/1‑140 (MQ=255)
                                                                          cGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTgcagca                                                             >  1:1481721/1‑140 (MQ=255)
                                                                          cGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTgcagca                                                             >  2:916025/1‑140 (MQ=255)
                                                                             ttCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGtt                                                          >  2:469429/1‑140 (MQ=255)
                                                                                gTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCaa                                                       >  1:737832/1‑140 (MQ=255)
                                                                                      aaTCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCAc                                                 <  2:453408/140‑1 (MQ=255)
                                                                                       aTCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACg                                                >  1:602717/1‑140 (MQ=255)
                                                                                       aTCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACg                                                >  2:317842/1‑140 (MQ=255)
                                                                                       aTCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACg                                                <  2:1180956/140‑1 (MQ=255)
                                                                                               aCGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTgc                                        >  2:1475766/1‑140 (MQ=255)
                                                                                               aCGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTgc                                        >  1:905311/1‑140 (MQ=255)
                                                                                                cgcgGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAGCTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTgcg                                       <  1:607926/140‑1 (MQ=255)
                                                                                                   ggTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCgcca                                    >  2:692377/1‑140 (MQ=255)
                                                                                                    gTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCgccag                                   >  1:50968/1‑140 (MQ=255)
                                                                                                    gTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGCTCAATGGCACGGCGTTTGCgccag                                   >  2:274470/1‑140 (MQ=255)
                                                                                                         ttCGCCAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCaaa                              >  2:1313572/1‑140 (MQ=255)
                                                                                                             ccAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGc                          >  1:1445027/1‑140 (MQ=255)
                                                                                                              cAGTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCt                         >  1:1424402/1‑140 (MQ=255)
                                                                                                                gTGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGc                       <  1:1327370/140‑1 (MQ=255)
                                                                                                                 tGCAATGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGcc                      >  1:1059873/1‑140 (MQ=255)
                                                                                                                     aTGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGccacca                  <  1:1313572/140‑1 (MQ=255)
                                                                                                                      tGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCaa                 >  1:1498594/1‑140 (MQ=255)
                                                                                                                      tGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCaa                 >  2:126911/1‑140 (MQ=255)
                                                                                                                      tGGCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCaa                 <  1:689118/140‑1 (MQ=255)
                                                                                                                       ggCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAAc                <  2:202681/140‑1 (MQ=255)
                                                                                                                       ggCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAAc                <  2:1481721/140‑1 (MQ=255)
                                                                                                                        gCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACg               <  1:42613/140‑1 (MQ=255)
                                                                                                                        gCTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACg               <  1:1028754/140‑1 (MQ=255)
                                                                                                                         cTATCATCACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACgg              <  2:737832/140‑1 (MQ=255)
                                                                                                                           atcatcACTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGcc            >  1:66090/1‑140 (MQ=255)
                                                                                                                             catcaCTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCtt          >  1:1493567/1‑140 (MQ=255)
                                                                                                                              atcaCTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCttt         >  1:1322221/1‑140 (MQ=255)
                                                                                                                               tcaCTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTta        <  2:1316022/140‑1 (MQ=255)
                                                                                                                               tcaCTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTta        <  1:83331/140‑1 (MQ=255)
                                                                                                                                caCTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACAGCCTTtat       <  2:1088207/140‑1 (MQ=255)
                                                                                                                                  cTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAg     >  2:461202/1‑140 (MQ=255)
                                                                                                                                  cTTTCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAg     >  1:1275116/1‑140 (MQ=255)
                                                                                                                                     tCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTc  >  2:365630/1‑140 (MQ=255)
                                                                                                                                     tCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTc  >  1:367993/1‑140 (MQ=255)
                                                                                                                                     tCTGGCATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTc  <  2:96776/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTC  >  NC_000913/1353000‑1353272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A