Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,438,355	T→C	T810T (ACA→ACG)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,438,355	0	T	C	98.6%	253.1 / ‑4.6	70	T810T (ACA→ACG)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (39/30); total (39/31)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

GCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAA  >  NC_000913/1438219‑1438491

gCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTa                                                                                                                                       <  2:966860/140‑1 (MQ=255)

  aGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAAt                                                                                                                                     >  2:832000/1‑140 (MQ=255)

  aGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAAt                                                                                                                                     >  2:677711/1‑140 (MQ=255)

    gTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCa                                                                                                                                   >  2:368081/1‑140 (MQ=255)

    gTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCa                                                                                                                                   >  1:156858/1‑140 (MQ=255)

        ccGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTg                                                                                                                               <  2:838994/140‑1 (MQ=255)

        ccGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTg                                                                                                                               <  1:472166/140‑1 (MQ=255)

         cGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGc                                                                                                                              >  1:62739/1‑140 (MQ=255)

          gCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCg                                                                                                                             <  2:616477/140‑1 (MQ=255)

              tAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGt                                                                                                                         >  2:849842/1‑140 (MQ=255)

              tAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGt                                                                                                                         >  2:376822/1‑140 (MQ=255)

              tAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGt                                                                                                                         >  2:1348820/1‑140 (MQ=255)

               aaaTTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTa                                                                                                                        <  1:1028623/140‑1 (MQ=255)

                 aTTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACg                                                                                                                      <  2:1305818/140‑1 (MQ=255)

                 aTTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACg                                                                                                                      <  1:677711/140‑1 (MQ=255)

                  ttGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGa                                                                                                                     >  2:106581/1‑140 (MQ=255)

                      agaaCAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAatat                                                                                                                 <  2:488803/140‑1 (MQ=255)

                          cAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAAt                                                                                                             <  2:809110/140‑1 (MQ=255)

                          cAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAAt                                                                                                             >  1:823530/1‑140 (MQ=255)

                             ccAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACg                                                                                                          >  2:358796/1‑140 (MQ=255)

                                gTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTc                                                                                                       <  2:1539703/140‑1 (MQ=255)

                                  ggCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCa                                                                                                     >  2:102139/1‑140 (MQ=255)

                                       tAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTg                                                                                                >  2:1253696/1‑140 (MQ=255)

                                           gATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTAc                                                                                            >  2:453833/1‑140 (MQ=255)

                                            aTCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACg                                                                                           >  1:329209/1‑140 (MQ=255)

                                                       gTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTg                                                                                >  2:432485/1‑140 (MQ=255)

                                                       gTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTg                                                                                >  2:865576/1‑140 (MQ=255)

                                                       gTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTg                                                                                <  2:823530/140‑1 (MQ=255)

                                                       gTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTg                                                                                >  1:332862/1‑140 (MQ=255)

                                                         cGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGc                                                                              <  2:62739/140‑1 (MQ=255)

                                                         cGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGc                                                                              <  1:63386/140‑1 (MQ=255)

                                                          gCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCa                                                                             <  1:368081/140‑1 (MQ=255)

                                                           ccATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAg                                                                            >  1:1133756/1‑140 (MQ=255)

                                                            cATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAgg                                                                           <  2:168142/140‑1 (MQ=255)

                                                            cATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAgg                                                                           <  1:1348820/140‑1 (MQ=255)

                                                                      aGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGgaaga                                                                 >  2:28112/1‑140 (MQ=255)

                                                                      aGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGgaaga                                                                 >  2:1032029/1‑140 (MQ=255)

                                                                      aGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGgaaga                                                                 >  1:449141/1‑140 (MQ=255)

                                                                      aGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGgaaga                                                                 >  2:1226550/1‑140 (MQ=255)

                                                                      aGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGgaaga                                                                 >  1:1050851/1‑140 (MQ=255)

                                                                      aGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGgaaga                                                                 >  1:1479904/1‑140 (MQ=255)

                                                                                ttAATATACGGCGTCTCGCCACAACCGGAGCAAGCACTTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAAtt                                                       >  2:1518273/1‑140 (MQ=255)

                                                                                      taCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGAtttt                                                 >  2:1473321/1‑140 (MQ=255)

                                                                                       aCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTtc                                                <  1:1253696/140‑1 (MQ=255)

                                                                                        cGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTtct                                               >  2:358757/1‑140 (MQ=255)

                                                                                          gCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTctct                                             >  1:461524/1‑140 (MQ=255)

                                                                                             tctcGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCtcttct                                          <  2:1386757/140‑1 (MQ=255)

                                                                                             tctcGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCtcttct                                          <  1:358796/140‑1 (MQ=255)

                                                                                              ctcGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTcttctt                                         >  1:1180279/1‑140 (MQ=255)

                                                                                                cGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCg                                       >  1:444882/1‑140 (MQ=255)

                                                                                                  ccACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGAc                                     <  1:832000/140‑1 (MQ=255)

                                                                                                  ccACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGAc                                     <  2:1133756/140‑1 (MQ=255)

                                                                                                   cacaACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACa                                    <  1:453833/140‑1 (MQ=255)

                                                                                                   cacaACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACa                                    <  1:196946/140‑1 (MQ=255)

                                                                                                   cacaACCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACa                                    <  1:432485/140‑1 (MQ=255)

                                                                                                       aCCGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGtt                                >  2:657989/1‑140 (MQ=255)

                                                                                                        ccGGAGCAAGCACCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTc                               <  2:156858/140‑1 (MQ=255)

                                                                                                              caagcaCCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCg                         <  1:849842/140‑1 (MQ=255)

                                                                                                              caagcaCCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCg                         <  1:529532/140‑1 (MQ=255)

                                                                                                                agcaCCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCgag                       >  2:675361/1‑140 (MQ=255)

                                                                                                                agcaCCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCgag                       <  1:443896/140‑1 (MQ=255)

                                                                                                                  caCCTGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGAc                     >  2:625975/1‑140 (MQ=255)

                                                                                                                      tGAATATTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGAcatca                 >  1:573702/1‑140 (MQ=255)

                                                                                                                           atTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTg            >  1:148145/1‑140 (MQ=255)

                                                                                                                           atTCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTg            >  1:48230/1‑140 (MQ=255)

                                                                                                                            ttCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGa           <  1:865576/140‑1 (MQ=255)

                                                                                                                            ttCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGa           <  1:386052/140‑1 (MQ=255)

                                                                                                                             tCAAACAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGAt          >  2:407351/1‑140 (MQ=255)

                                                                                                                               aaaCAGCGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATgg        >  2:1487025/1‑140 (MQ=255)

                                                                                                                                    gcggcgTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTa   >  2:218076/1‑140 (MQ=255)

                                                                                                                                     cGGCGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTaa  <  1:1226550/140‑1 (MQ=255)

GCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAA  >  NC_000913/1438219‑1438491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A