Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 162 93.0 3098984 99.0% 3067994 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,015,321 T→C L148L (TTG→CTG)  fliG → flagellar motor switch protein FliG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,015,3210TC98.5% 239.4 / ‑4.4 67L148L (TTG→CTG) fliGflagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (36/30);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGG  >  NC_000913/2015193‑2015460
                                                                                                                                |                                                                                                                                           
gCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGaa                                                                                                                                  >  2:917794/1‑140 (MQ=255)
  ggTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACg                                                                                                                                >  2:1142960/1‑140 (MQ=255)
     aTTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCt                                                                                                                             <  1:1008803/140‑1 (MQ=255)
         aaaCGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTgcgc                                                                                                                         <  2:906085/140‑1 (MQ=255)
          aaCGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGcc                                                                                                                        <  2:459756/140‑1 (MQ=255)
           aCGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGccc                                                                                                                       >  2:1542969/1‑139 (MQ=255)
           aCGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCCGCGccc                                                                                                                       >  2:1409938/1‑139 (MQ=255)
           aCGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGAATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCa                                                                                                                       <  2:1441366/140‑1 (MQ=255)
              cTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCacga                                                                                                                    >  2:540344/1‑140 (MQ=255)
              cTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCacga                                                                                                                    >  1:387494/1‑140 (MQ=255)
              cTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCacga                                                                                                                    >  1:179464/1‑140 (MQ=255)
              cTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCacga                                                                                                                    >  2:1019031/1‑140 (MQ=255)
              cTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCacga                                                                                                                    >  2:525980/1‑140 (MQ=255)
              cTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCacga                                                                                                                    >  1:144378/1‑140 (MQ=255)
              cTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCacga                                                                                                                    >  1:1164083/1‑140 (MQ=255)
               tCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCTACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCacgac                                                                                                                   <  2:146906/140‑1 (MQ=255)
                   cTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGa                                                                                                               >  1:1196558/1‑140 (MQ=255)
                           aGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGt                                                                                                       >  1:337703/1‑140 (MQ=255)
                             ccACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTAt                                                                                                     >  2:1092258/1‑140 (MQ=255)
                             ccACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTAt                                                                                                     <  1:941461/140‑1 (MQ=255)
                              cacaGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATc                                                                                                    <  1:816959/140‑1 (MQ=255)
                                caGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGc                                                                                                  >  2:1210878/1‑140 (MQ=255)
                                  gagCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCa                                                                                                >  1:1220063/1‑140 (MQ=255)
                                     cgccgcCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCt                                                                                             >  2:1043327/1‑140 (MQ=255)
                                     cgccgcCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCt                                                                                             >  1:1231644/1‑140 (MQ=255)
                                        cgccgATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTg                                                                                          >  2:1329759/1‑140 (MQ=255)
                                        cgccgATCTGATACGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTg                                                                                          >  1:710618/1‑140 (MQ=255)
                                          ccgATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTggc                                                                                        <  2:1487537/140‑1 (MQ=255)
                                          ccgATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTggc                                                                                        <  1:917794/140‑1 (MQ=255)
                                          ccgATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTggc                                                                                        >  2:1528822/1‑140 (MQ=255)
                                          ccgATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTggc                                                                                        <  2:862043/140‑1 (MQ=255)
                                           cgATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTggcg                                                                                       <  1:1142960/140‑1 (MQ=255)
                                                        gATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGcagccagc                                                                          <  1:547830/140‑1 (MQ=255)
                                                        gATGAGCATCCGCAAATTATCGCCACCATTCTGGTGAATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGcagccagc                                                                          <  2:144378/140‑1 (MQ=255)
                                                            aGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCcgcg                                                                      >  1:1492600/1‑140 (MQ=255)
                                                            aGCATCCGCAAATTATCACCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCcgcg                                                                      >  2:779710/1‑140 (MQ=255)
                                                                tCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTgg                                                                  >  2:899473/1‑140 (MQ=255)
                                                                tCCGCAAATTATCGCCACCATCCTGGTGCATCTGAGGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAGCGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTgg                                                                  <  2:1468803/140‑1 (MQ=255)
                                                                 ccGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGc                                                                 >  2:1047809/1‑140 (MQ=255)
                                                                 ccGCAAATTATCGCCACCATTCTGGT‑CATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCg                                                                >  1:1158782/1‑140 (MQ=255)
                                                                  cGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCg                                                                <  2:179464/140‑1 (MQ=255)
                                                                     aaaTTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAg                                                             <  2:1127912/140‑1 (MQ=255)
                                                                     aaaTTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAg                                                             <  2:1231644/140‑1 (MQ=255)
                                                                       aTTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCt                                                           <  1:1528822/140‑1 (MQ=255)
                                                                         tATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGa                                                         <  1:1019031/140‑1 (MQ=255)
                                                                         tATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGAAGCCAGCCGCGCTGGCGGAGCTGa                                                         <  2:1220063/140‑1 (MQ=255)
                                                                         tATCGCCACCATTCGGGTGCATCTGAAGCGCGCCGAAGCCGCCGATCTTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGa                                                         <  2:623321/140‑1 (MQ=255)
                                                                            cGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCg                                                      <  1:1025672/140‑1 (MQ=255)
                                                                            cGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCg                                                      <  1:906217/140‑1 (MQ=255)
                                                                            cGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCg                                                      <  1:1329759/140‑1 (MQ=255)
                                                                            cGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCg                                                      <  1:540344/140‑1 (MQ=255)
                                                                              ccaccaTTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGaa                                                    <  2:337703/140‑1 (MQ=255)
                                                                                 ccaTTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTa                                                 <  2:1325965/140‑1 (MQ=255)
                                                                                 ccaTTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTa                                                 <  2:310429/140‑1 (MQ=255)
                                                                                     tCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGa                                             <  2:1164083/140‑1 (MQ=255)
                                                                                     tCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGa                                             <  2:387494/140‑1 (MQ=255)
                                                                                         gTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATgg                                         >  2:589072/1‑140 (MQ=255)
                                                                                              tCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGc                                    >  1:1319916/1‑140 (MQ=255)
                                                                                              tCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGc                                    >  1:267285/1‑140 (MQ=255)
                                                                                              tCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCCTGc                                    >  2:1270000/1‑140 (MQ=255)
                                                                                                     cgcgcCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACgg                             <  2:1081905/140‑1 (MQ=255)
                                                                                                       cgcCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTc                           >  1:1453633/1‑140 (MQ=255)
                                                                                                       cgcCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTc                           <  1:626427/140‑1 (MQ=255)
                                                                                                       cgcCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTc                           >  2:896869/1‑140 (MQ=255)
                                                                                                       cgcCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTc                           >  2:926171/1‑140 (MQ=255)
                                                                                                        gcCCAAGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCa                          >  2:46790/1‑140 (MQ=255)
                                                                                                          ccATGCCGCCGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGa                        <  1:1273218/140‑1 (MQ=255)
                                                                                                              gccgccGATATTCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAAtct                    >  1:747335/1‑140 (MQ=255)
                                                                                                                        ttCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCgcagc          >  1:979854/1‑140 (MQ=255)
                                                                                                                         tCTGGCGCTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCgcagca         >  2:1447149/1‑140 (MQ=255)
                                                                                                                             gcgcTGTTCGATGAACGTCTGCGTCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAAt     >  1:1163705/1‑140 (MQ=255)
                                                                                                                                ttgttCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATggg  <  1:1047809/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                           
GCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCGATGAACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGG  >  NC_000913/2015193‑2015460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A