Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,536,991	G→A	R83C (CGT→TGT)	pdxK ←	pyridoxal kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,536,991	0	G	A	98.8%	281.2 / NA	86	R83C (CGT→TGT)	pdxK	pyridoxal kinase I
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (51/34); minor base C (0/1); total (51/35)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATA  >  NC_000913/2536855‑2537124

aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  2:761567/1‑140 (MQ=255)

aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  2:442883/1‑140 (MQ=255)

aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  2:432533/1‑140 (MQ=255)

aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  1:46829/1‑140 (MQ=255)

aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  1:815900/1‑140 (MQ=255)

   aCCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTg                                                                                                                                 <  2:1329306/140‑1 (MQ=255)

    ccGGATCGACCATGTTCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGa                                                                                                                                >  1:88976/1‑140 (MQ=255)

      ggATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAg                                                                                                                              <  1:323402/140‑1 (MQ=255)

       gATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAgc                                                                                                                             >  1:17346/1‑140 (MQ=255)

       gATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGCTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAgc                                                                                                                             >  1:464679/1‑140 (MQ=255)

          cGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgca                                                                                                                          <  1:1230183/140‑1 (MQ=255)

           gACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgcac                                                                                                                         >  1:797272/1‑140 (MQ=255)

            aCCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgcacg                                                                                                                        <  2:1393366/140‑1 (MQ=255)

            aCCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgcacg                                                                                                                        >  1:1127215/1‑140 (MQ=255)

            aCCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgcacg                                                                                                                        >  2:526407/1‑140 (MQ=255)

                  aTCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATa                                                                                                                  >  2:334412/1‑140 (MQ=255)

                  aTCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATa                                                                                                                  >  2:1273546/1‑140 (MQ=255)

                       tAGGTCAGGATGGTCTTTGCGTAGCGCGGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGc                                                                                                             >  2:847981/1‑140 (MQ=255)

                       tAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGc                                                                                                             >  1:852339/1‑140 (MQ=255)

                            cAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAAc                                                                                                        >  1:487749/1‑140 (MQ=255)

                              ggATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGTCGCTAAACCa                                                                                                      >  2:1195772/1‑140 (MQ=255)

                               gATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCAt                                                                                                     <  2:514073/140‑1 (MQ=255)

                               gATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCAt                                                                                                     >  1:997022/1‑140 (MQ=255)

                               gATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCAt                                                                                                     >  1:571178/1‑140 (MQ=255)

                               gATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTCGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCAt                                                                                                     <  2:1251439/140‑1 (MQ=255)

                                    tCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTc                                                                                                >  1:1112319/1‑140 (MQ=255)

                                    tCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTc                                                                                                >  1:804586/1‑140 (MQ=255)

                                         gCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGaa                                                                                           >  2:75011/1‑140 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:424512/140‑1 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  2:1077160/140‑1 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:99835/140‑1 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  2:84376/140‑1 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:1232858/140‑1 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:128139/140‑1 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:174662/140‑1 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      >  2:615937/1‑140 (MQ=255)

                                              gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  2:46829/140‑1 (MQ=255)

                                               cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcac                                                                                     <  2:350463/140‑1 (MQ=255)

                                               cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcac                                                                                     >  1:543206/1‑140 (MQ=255)

                                               cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcac                                                                                     <  2:88976/140‑1 (MQ=255)

                                               cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  2:337877/139‑1 (MQ=255)

                                               cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      >  1:337877/1‑139 (MQ=255)

                                                gcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcacc                                                                                    <  1:243099/140‑1 (MQ=255)

                                                gcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCAGAATCGcacc                                                                                    <  1:542711/140‑1 (MQ=255)

                                                  aGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcaccac                                                                                  >  1:838522/1‑140 (MQ=255)

                                                   gTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCaccacc                                                                                 <  1:432533/140‑1 (MQ=255)

                                                    tCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCg                                                                                >  1:899125/1‑140 (MQ=255)

                                                      aGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTa                                                                              <  2:258177/140‑1 (MQ=255)

                                                        ccACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGa                                                                            <  2:78605/140‑1 (MQ=255)

                                                         cACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa                                                                           >  1:1108736/1‑140 (MQ=255)

                                                            tCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAgt                                                                        >  1:101821/1‑140 (MQ=255)

                                                                    gATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAAt                                                                >  1:409492/1‑140 (MQ=255)

                                                                     aTTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATg                                                               >  2:1142703/1‑140 (MQ=255)

                                                                     aTTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATg                                                               <  1:761567/140‑1 (MQ=255)

                                                                       tttGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcg                                                             <  1:75011/140‑1 (MQ=255)

                                                                             ttGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTAt                                                       <  2:674451/140‑1 (MQ=255)

                                                                                 gATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCt                                                   <  2:1246637/140‑1 (MQ=255)

                                                                                 gATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCt                                                   <  2:804586/140‑1 (MQ=255)

                                                                                 gATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCt                                                   <  1:334412/140‑1 (MQ=255)

                                                                                      cGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCa                                              <  1:615937/140‑1 (MQ=255)

                                                                                        ttCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAAt                                            >  1:36203/1‑140 (MQ=255)

                                                                                             atatAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGt                                       <  2:852339/140‑1 (MQ=255)

                                                                                             atatAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGt                                       >  2:1044479/1‑140 (MQ=255)

                                                                                               ataGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcg                                     >  2:455241/1‑140 (MQ=255)

                                                                                               ataGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcg                                     >  1:1415647/1‑140 (MQ=255)

                                                                                                taGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcgg                                    <  1:1195772/140‑1 (MQ=255)

                                                                                                 aGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggc                                   <  1:694536/140‑1 (MQ=255)

                                                                                                  gCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggca                                  >  2:309862/1‑140 (MQ=255)

                                                                                                  gCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggca                                  <  2:769480/140‑1 (MQ=255)

                                                                                                  gCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggca                                  >  2:223582/1‑140 (MQ=255)

                                                                                                   cccGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  2:1362490/1‑140 (MQ=255)

                                                                                                   cccGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  1:437357/1‑140 (MQ=255)

                                                                                                   cccGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  1:34062/1‑140 (MQ=255)

                                                                                                   cccGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGGCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  2:1359983/1‑140 (MQ=255)

                                                                                                   cccGTGGTTACAGCACGAAGTTGGCGCAGCACATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  1:736586/1‑140 (MQ=255)

                                                                                                     cGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcacgg                               >  2:601215/1‑140 (MQ=255)

                                                                                                     cGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcacgg                               >  1:1302756/1‑140 (MQ=255)

                                                                                                     cGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcacgg                               >  2:1280967/1‑140 (MQ=255)

                                                                                                                cACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTc                    >  2:1113414/1‑140 (MQ=255)

                                                                                                                  cGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAg                  >  2:1269286/1‑140 (MQ=255)

                                                                                                                   gAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAgg                 <  1:1142703/140‑1 (MQ=255)

                                                                                                                   gAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCCTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAgg                 >  2:1515893/1‑140 (MQ=255)

                                                                                                                    aaGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGc                >  2:663690/1‑140 (MQ=255)

                                                                                                                          gcgcagcgcaTCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCt          >  1:115545/1‑140 (MQ=255)

                                                                                                                               gcgcaTCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTg     >  1:299250/1‑140 (MQ=255)

                                                                                                                                cgcaTCACCCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGa    <  2:1127215/140‑1 (MQ=255)

                                                                                                                                cgcaTCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGa    >  1:579515/1‑140 (MQ=255)

                                                                                                                                  caTCACCCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATa  <  2:997022/140‑1 (MQ=255)

ATCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATA  >  NC_000913/2536855‑2537124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A