Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,993,351	C→T	P420S (CCT→TCT)	ygeH →	putative transcriptional regulator YgeH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,993,351	0	C	T	100.0%	282.8 / NA	81	P420S (CCT→TCT)	ygeH	putative transcriptional regulator YgeH
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (40/41); total (40/41)

TCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATAAATTTTTATTGACG  >  NC_000913/2993214‑2993483

tctACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCt                                                                                                                                    >  2:1007218/1‑140 (MQ=255)

tctACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCt                                                                                                                                    >  2:1224567/1‑140 (MQ=255)

       ctaTAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGc                                                                                                                             >  1:1513117/1‑140 (MQ=255)

          taGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCa                                                                                                                          >  2:538384/1‑140 (MQ=255)

          taGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCa                                                                                                                          >  2:501243/1‑140 (MQ=255)

          taGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCa                                                                                                                          >  2:897508/1‑140 (MQ=255)

           aGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCaa                                                                                                                         <  2:744678/140‑1 (MQ=255)

            gggCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCaaa                                                                                                                        >  2:1371833/1‑140 (MQ=255)

            gggCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCaaa                                                                                                                        >  2:1500083/1‑140 (MQ=255)

            gggCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCaaa                                                                                                                        >  1:1272006/1‑140 (MQ=255)

              gCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCaaaaa                                                                                                                      >  1:1278204/1‑140 (MQ=255)

              gCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCaaaaa                                                                                                                      >  1:46493/1‑140 (MQ=255)

               cACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAaac                                                                                                                     >  2:1450677/1‑140 (MQ=255)

               cACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAaac                                                                                                                     >  1:1198178/1‑140 (MQ=255)

               cACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAaac                                                                                                                     >  2:252941/1‑140 (MQ=255)

                  tAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAaacaac                                                                                                                  >  1:791966/1‑140 (MQ=255)

                   aGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAacaaca                                                                                                                 <  2:606373/140‑1 (MQ=255)

                   aGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAacaaca                                                                                                                 <  1:1084930/140‑1 (MQ=255)

                    gTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACAt                                                                                                                >  2:549402/1‑140 (MQ=255)

                    gTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACAt                                                                                                                >  1:226800/1‑140 (MQ=255)

                       cACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTaa                                                                                                             >  1:1514826/1‑140 (MQ=255)

                         cTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAAc                                                                                                           >  1:1399113/1‑140 (MQ=255)

                          tttCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAAct                                                                                                          <  1:338963/140‑1 (MQ=255)

                           ttCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAActc                                                                                                         <  1:114968/140‑1 (MQ=255)

                            tCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAActct                                                                                                        >  1:230336/1‑140 (MQ=255)

                              aTAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTctac                                                                                                      >  2:869126/1‑140 (MQ=255)

                              aTAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTctac                                                                                                      >  2:234700/1‑140 (MQ=255)

                               tAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTctact                                                                                                     >  1:1164683/1‑140 (MQ=255)

                                aaTGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTctacta                                                                                                    <  1:1007218/140‑1 (MQ=255)

                                  tGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACtata                                                                                                  <  2:1342482/140‑1 (MQ=255)

                                  tGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGCGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACtata                                                                                                  <  1:382195/140‑1 (MQ=255)

                                    aaaaaaTTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATaaa                                                                                                >  2:791714/1‑140 (MQ=255)

                                    aaaaaaTTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATaaa                                                                                                >  2:577305/1‑140 (MQ=255)

                                        aaTTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGaaa                                                                                            <  2:844912/140‑1 (MQ=255)

                                            gAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTga                                                                                        <  1:1500083/140‑1 (MQ=255)

                                            gAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTga                                                                                        <  2:467038/140‑1 (MQ=255)

                                              agagGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTgaga                                                                                      <  2:1513117/140‑1 (MQ=255)

                                                  gCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGa                                                                                  <  1:1371833/140‑1 (MQ=255)

                                                  gCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGa                                                                                  <  1:1224567/140‑1 (MQ=255)

                                                    aaGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGaaa                                                                                >  2:420766/1‑140 (MQ=255)

                                                        aTTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCa                                                                            <  2:398939/140‑1 (MQ=255)

                                                        aTTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCa                                                                            <  2:935411/140‑1 (MQ=255)

                                                         tttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCAt                                                                           <  2:833712/140‑1 (MQ=255)

                                                         tttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCAt                                                                           <  2:822340/140‑1 (MQ=255)

                                                         tttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCAt                                                                           <  2:230336/140‑1 (MQ=255)

                                                         tttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCAt                                                                           <  2:1510571/140‑1 (MQ=255)

                                                         tttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCAt                                                                           <  1:1051724/140‑1 (MQ=255)

                                                         tttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCAt                                                                           <  2:1278204/140‑1 (MQ=255)

                                                         tttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCAt                                                                           <  1:1450677/140‑1 (MQ=255)

                                                         tttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCAt                                                                           <  1:869126/140‑1 (MQ=255)

                                                           tGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCg                                                                         >  2:935455/1‑140 (MQ=255)

                                                           tGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCg                                                                         <  1:897508/140‑1 (MQ=255)

                                                           tGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAA‑‑CTACTATAAAGAAACTGAGAGTGAAAGCCATCGAg                                                                       <  2:1153173/140‑1 (MQ=255)

                                                             tataGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAg                                                                       <  1:342199/140‑1 (MQ=255)

                                                             tataGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAg                                                                       <  2:204308/140‑1 (MQ=255)

                                                                aGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTta                                                                    <  1:702626/140‑1 (MQ=255)

                                                                 gACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTtat                                                                   >  1:121660/1‑140 (MQ=255)

                                                                 gACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTtat                                                                   >  2:1526267/1‑140 (MQ=255)

                                                                  aCAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTtata                                                                  >  2:1013444/1‑140 (MQ=255)

                                                                      aTCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTg                                                              >  2:259341/1‑140 (MQ=255)

                                                                       tCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGa                                                             >  2:1154814/1‑140 (MQ=255)

                                                                       tCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGa                                                             >  1:423219/1‑140 (MQ=255)

                                                                                cTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACAtttt                                                    <  2:445992/140‑1 (MQ=255)

                                                                                  cGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGa                                                  <  1:336595/140‑1 (MQ=255)

                                                                                          gaagaCGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAag                                          <  2:746156/140‑1 (MQ=255)

                                                                                           aagaCGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAagc                                         >  2:336062/1‑140 (MQ=255)

                                                                                              aCGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAagcagc                                      <  2:226800/140‑1 (MQ=255)

                                                                                                  aaaGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGAc                                  <  2:1272006/140‑1 (MQ=255)

                                                                                                    aGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACga                                <  1:420766/140‑1 (MQ=255)

                                                                                                          tgtgATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAAttt                          >  2:784591/1‑140 (MQ=255)

                                                                                                             gATAAAAGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTa                       <  1:259341/140‑1 (MQ=255)

                                                                                                                  aaGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGa                  <  2:601355/140‑1 (MQ=255)

                                                                                                                   aGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGAt                 >  1:370621/1‑140 (MQ=255)

                                                                                                                   aGAATGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGAt                 <  1:1526267/140‑1 (MQ=255)

                                                                                                                      aTGTGTAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATaaa              >  2:1367132/1‑140 (MQ=255)

                                                                                                                        gtgtAGATATGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATAAAtt            >  2:1319416/1‑140 (MQ=255)

                                                                                                                              atatGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATAAATTTTTAtt      >  1:522957/1‑140 (MQ=255)

                                                                                                                              atatGTATGTGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATAAATTTCTAtt      >  1:664370/1‑140 (MQ=255)

                                                                                                                                  gtatgtGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATAAATTTTTATTGACg  <  1:577305/140‑1 (MQ=255)

                                                                                                                                  gtatgtGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATAAATTTTTATTGACg  <  1:791944/140‑1 (MQ=255)

                                                                                                                                  gtatgtGTCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATAAATTTTTATTGACg  <  2:423219/140‑1 (MQ=255)

TCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAGTTATAATTGACAACATTTTGAAATTAAAGCAGCTGACGAGAATTTGTATGCGATAAATTTTTATTGACG  >  NC_000913/2993214‑2993483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A