Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,363	G→A	intergenic (‑230/+150)	metT ← / ← asnB	tRNA‑Met/asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,363	0	G	A	100.0%	207.2 / NA	57	intergenic (‑230/+150)	metT/asnB	tRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/35); total (22/35)

GAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCG  >  NC_000913/697227‑697495

gAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGaaaa                                                                                                                                   <  1:1053839/140‑1 (MQ=255)

     tGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAAt                                                                                                                              <  1:709099/140‑1 (MQ=255)

     tGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAAt                                                                                                                              <  2:781764/140‑1 (MQ=255)

            ttGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATaaa                                                                                                                       >  1:856787/1‑140 (MQ=255)

               gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATa                                                                                                                    <  2:891808/140‑1 (MQ=255)

               gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATa                                                                                                                    <  2:369124/140‑1 (MQ=255)

               gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATa                                                                                                                    <  2:376897/140‑1 (MQ=255)

               gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATa                                                                                                                    <  2:132526/140‑1 (MQ=255)

               gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATAAAATa                                                                                                                    <  2:856787/140‑1 (MQ=255)

                    gTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAACATTAATCTATGAAATACTGGc                                                                                                               <  2:875207/140‑1 (MQ=255)

                     ttGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGcc                                                                                                              >  2:284278/1‑140 (MQ=255)

                      tGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCt                                                                                                             <  1:667200/140‑1 (MQ=255)

                       gCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTa                                                                                                            <  2:210848/140‑1 (MQ=255)

                       gCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTa                                                                                                            >  1:438846/1‑140 (MQ=255)

                       gCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTa                                                                                                            <  1:1138232/140‑1 (MQ=255)

                       gCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTa                                                                                                            <  1:989982/140‑1 (MQ=255)

                         gAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACa                                                                                                          <  2:661553/140‑1 (MQ=255)

                          aaCAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAg                                                                                                         <  1:923857/140‑1 (MQ=255)

                              gCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGtgat                                                                                                     >  1:463056/1‑140 (MQ=255)

                                  ggCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGt                                                                                                 <  1:961321/140‑1 (MQ=255)

                                  ggCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGt                                                                                                 >  1:909345/1‑140 (MQ=255)

                                    cgcTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTg                                                                                               <  1:284278/140‑1 (MQ=255)

                                       tataTTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCa                                                                                            <  2:150589/140‑1 (MQ=255)

                                         tatTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCaaa                                                                                          <  1:56418/140‑1 (MQ=255)

                                               tCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGa                                                                                    >  1:848915/1‑140 (MQ=255)

                                                cAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGAt                                                                                   <  2:1096688/140‑1 (MQ=255)

                                                 aaTTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAg                                                                                                  >  1:47350/1‑124 (MQ=255)

                                                 aaTTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAg                                                                                                  <  2:47350/124‑1 (MQ=255)

                                                   ttattGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCa                                                                                            >  2:1205616/1‑128 (MQ=255)

                                                   ttattGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCa                                                                                            <  1:1205616/128‑1 (MQ=255)

                                                      ttGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCa                                                                             <  1:156952/140‑1 (MQ=255)

                                                             cTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCgg                                                                      >  1:1048345/1‑140 (MQ=255)

                                                                tgtgTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAAc                                                                   >  2:210684/1‑140 (MQ=255)

                                                                      ttttCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTcc                                                             <  2:1048345/140‑1 (MQ=255)

                                                                            cACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTa                                                          >  1:496810/1‑137 (MQ=255)

                                                                            cACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTa                                                          <  2:496810/137‑1 (MQ=255)

                                                                              cccTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCa                                                     >  2:875662/1‑140 (MQ=255)

                                                                                 tACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCatat                                                  >  1:132533/1‑140 (MQ=255)

                                                                                    aGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAg                                               <  2:132533/140‑1 (MQ=255)

                                                                                    aGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAg                                               <  2:473063/140‑1 (MQ=255)

                                                                                         ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTGCAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAAt                                            >  2:1073629/1‑138 (MQ=255)

                                                                                         ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAAt                                            <  1:1073629/138‑1 (MQ=255)

                                                                                         ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATca                                          >  2:234961/1‑140 (MQ=255)

                                                                                         ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATca                                          >  2:299614/1‑140 (MQ=255)

                                                                                         ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATca                                          >  2:314465/1‑140 (MQ=255)

                                                                                         ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATca                                          >  2:1207859/1‑140 (MQ=255)

                                                                                          tCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaa                                         >  2:169069/1‑140 (MQ=255)

                                                                                          tCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaa                                         >  2:710044/1‑140 (MQ=255)

                                                                                           cTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaac                                        <  2:188043/140‑1 (MQ=255)

                                                                                            ttttGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaaca                                       <  1:314465/140‑1 (MQ=255)

                                                                                              ttGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAg                                     <  1:1207859/140‑1 (MQ=255)

                                                                                               tGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGc                                    <  1:875662/140‑1 (MQ=255)

                                                                                                     aCAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCa                              <  1:299614/140‑1 (MQ=255)

                                                                                                                   ggAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCa                              >  2:212892/1‑126 (MQ=255)

                                                                                                                   ggAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCa                              <  1:212892/126‑1 (MQ=255)

                                                                                                                          aaCAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGAt         <  2:909345/140‑1 (MQ=255)

                                                                                                                            cAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGAttt       <  1:169069/140‑1 (MQ=255)

                                                                                                                                 gtggtgGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCg  >  2:104580/1‑140 (MQ=255)

GAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCG  >  NC_000913/697227‑697495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A